Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NWAT_RS02010 Genome accession   NC_014315
Coordinates   421977..423692 (-) Length   571 a.a.
NCBI ID   WP_041350397.1    Uniprot ID   -
Organism   Nitrosococcus watsonii C-113     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 416977..428692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NWAT_RS01975 (Nwat_0400) - 417514..417735 (+) 222 WP_013219477.1 zinc-finger domain-containing protein -
  NWAT_RS01980 (Nwat_0401) - 417808..418008 (-) 201 WP_013219478.1 DUF2905 domain-containing protein -
  NWAT_RS01985 (Nwat_0402) - 418052..419005 (+) 954 WP_013219479.1 Nudix family hydrolase -
  NWAT_RS01990 (Nwat_0403) yacG 419013..419201 (-) 189 WP_013219480.1 DNA gyrase inhibitor YacG -
  NWAT_RS01995 (Nwat_0404) coaE 419207..419857 (-) 651 WP_013219481.1 dephospho-CoA kinase -
  NWAT_RS02000 (Nwat_0405) pilD 419866..420732 (-) 867 WP_013219482.1 A24 family peptidase Machinery gene
  NWAT_RS02005 (Nwat_0406) pilC 420752..421972 (-) 1221 WP_013219483.1 type II secretion system F family protein Machinery gene
  NWAT_RS02010 (Nwat_0407) pilB 421977..423692 (-) 1716 WP_041350397.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NWAT_RS17950 lptM 423936..424034 (+) 99 WP_157679984.1 LPS translocon maturation chaperone LptM -
  NWAT_RS02015 (Nwat_0408) lysA 424038..425288 (+) 1251 WP_198342191.1 diaminopimelate decarboxylase -
  NWAT_RS02020 (Nwat_0409) - 425339..426010 (+) 672 WP_013219486.1 class I SAM-dependent methyltransferase -
  NWAT_RS02025 (Nwat_0410) dapF 426050..426880 (+) 831 WP_013219487.1 diaminopimelate epimerase -
  NWAT_RS02030 (Nwat_0411) - 426877..427608 (+) 732 WP_013219488.1 DUF484 family protein -
  NWAT_RS02035 (Nwat_0412) xerC 427616..428518 (+) 903 WP_013219489.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62984.25 Da        Isoelectric Point: 5.7098

>NTDB_id=37764 NWAT_RS02010 WP_041350397.1 421977..423692(-) (pilB) [Nitrosococcus watsonii C-113]
MATPGVQIIMSGLVRRLVEDGLLSEANARQAHEHSRKSGTPLVTYLVQQKLLKSLDIGQAASQEFGVPLFDINALDLDYL
PKGLVEEKLVRQHQALPLFKRGNRLFVAVADPTNLQALDEIKFHTGINTEAILVEEDKLARMIERAMEAQDTSLADLNDT
ALDDLDISGGENEPPENSAESEADDTPVVRFINKVLLDAIHQGASDIHFEPYEKIYRIRYRQDGVLREVATPPIALSGRL
AARLKVMSRLDISERRVPQDGRMKMNISKNRAIDFRVNSCPTLFGEKIVLRILDPSSAQMGIEALGYEERQQQLFLETIH
RPYGMVLVTGPTGSGKTVSLYTALNILNTADRNISTAEDPAEINLPGINQVNVYPKVGLTFAGALKAFLRQDPDVIMVGE
IRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTLTRLLNMGVASYNIASAVSLIIAQRLARRLCSRCKTPEKIPHEALLEE
GFTQAQLETTPTLYKAVGCEHCTKGYKGRVGIYQVMPVSEEMGRIIMAGGNSMELAEQSKKEGVADLRQSGLKKIIDGMT
SIEEINRVTKE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=37764 NWAT_RS02010 WP_041350397.1 421977..423692(-) (pilB) [Nitrosococcus watsonii C-113]
ATGGCCACTCCTGGAGTACAGATCATAATGAGCGGTCTGGTCCGCCGTTTGGTAGAGGATGGATTGCTTAGTGAAGCCAA
TGCCCGGCAAGCCCACGAGCATTCCCGTAAAAGCGGCACTCCTTTGGTGACTTACCTGGTACAGCAAAAGCTACTTAAAA
GCCTGGATATTGGTCAAGCAGCCTCCCAAGAATTCGGCGTCCCTCTCTTCGATATCAATGCCTTGGATTTGGATTATCTT
CCCAAAGGACTCGTGGAAGAAAAATTGGTTCGCCAACATCAGGCACTACCCTTATTCAAGCGTGGAAATCGTCTCTTCGT
TGCCGTGGCCGATCCCACCAACCTGCAAGCATTAGACGAAATCAAGTTCCATACGGGAATTAATACCGAAGCTATCCTCG
TTGAAGAAGACAAGCTAGCACGCATGATCGAGCGGGCCATGGAGGCCCAAGACACTTCCCTGGCAGACCTAAACGATACG
GCGCTAGATGACCTTGACATCTCTGGAGGTGAAAATGAGCCTCCGGAAAATTCTGCTGAATCGGAGGCTGATGATACCCC
GGTAGTTCGTTTTATTAATAAAGTTCTGTTGGACGCCATTCATCAGGGAGCCTCGGATATTCATTTTGAGCCCTATGAAA
AAATCTATCGGATCAGATACCGGCAAGATGGGGTGTTGCGGGAAGTCGCCACCCCTCCTATTGCCCTGTCCGGTCGGTTG
GCAGCCCGCCTCAAAGTCATGTCCCGCTTGGATATTTCCGAACGGAGGGTACCTCAAGATGGGCGCATGAAAATGAATAT
TTCCAAAAATCGCGCCATTGATTTTCGGGTAAATAGTTGCCCCACTTTATTCGGCGAGAAAATCGTATTGCGTATCCTGG
ACCCCTCCAGCGCGCAAATGGGAATCGAGGCGCTAGGTTATGAAGAAAGACAACAACAACTCTTTCTAGAAACCATTCAC
CGCCCTTATGGGATGGTGCTCGTTACCGGCCCCACGGGCAGTGGTAAAACGGTCTCTCTTTATACTGCGCTTAATATTCT
TAATACCGCGGACCGTAATATCTCCACTGCCGAAGACCCAGCGGAAATCAATCTGCCTGGTATTAACCAAGTCAATGTCT
ACCCCAAAGTCGGTTTGACTTTCGCCGGCGCCCTTAAAGCCTTCTTGCGTCAGGACCCCGATGTCATCATGGTGGGGGAA
ATTCGGGATCTGGAAACGGCTGAAATTGCCATTAAAGCTGCCCAAACCGGCCATATGGTGCTTTCCACCCTTCATACCAA
CGACGCGCCCCAAACCCTGACCCGGCTATTAAACATGGGGGTGGCGTCCTACAACATCGCCTCGGCAGTTTCTTTGATCA
TCGCCCAACGCCTGGCCCGGCGCCTCTGCTCCCGCTGCAAAACACCCGAAAAGATACCCCACGAAGCCCTGCTAGAAGAA
GGTTTTACCCAAGCTCAATTGGAAACCACGCCCACCCTTTATAAGGCAGTAGGCTGCGAGCACTGCACCAAAGGCTATAA
AGGCCGCGTGGGTATCTATCAGGTCATGCCTGTATCAGAGGAGATGGGCCGCATTATTATGGCGGGAGGCAATTCCATGG
AACTTGCGGAGCAGTCAAAAAAAGAAGGCGTCGCCGATCTCCGCCAATCGGGGCTTAAAAAAATTATTGATGGCATGACC
AGTATAGAAGAAATTAACCGTGTAACGAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.801

98.774

0.571
  pilB Acinetobacter baylyi ADP1

57.042

99.475

0.567
  pilB Legionella pneumophila strain ERS1305867

56.714

99.124

0.562
  pilF Neisseria gonorrhoeae MS11

51.832

100

0.52
  pilB Vibrio cholerae strain A1552

49.206

99.299

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

48.85

98.949

0.483
  pilB Vibrio campbellii strain DS40M4

48.233

99.124

0.478
  pilF Thermus thermophilus HB27

39.459

97.198

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.571

98.074

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

39.884

90.893

0.363

Multiple sequence alignment