Detailed information NTG0037762

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NWAT_RS02000
• Genome accession: NC_014315
• Coordinates: 419866..420732 (-)
• Length: 288 a.a.
• NCBI ID: WP_013219482.1
• Uniprot ID: D8KA57
• Organism: Nitrosococcus watsonii C-113
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 414866..425732

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NWAT_RS01970 (Nwat_0399)	nadA	416358..417455 (+)	1098	WP_013219476.1	quinolinate synthase NadA	-
NWAT_RS01975 (Nwat_0400)	-	417514..417735 (+)	222	WP_013219477.1	zinc-finger domain-containing protein	-
NWAT_RS01980 (Nwat_0401)	-	417808..418008 (-)	201	WP_013219478.1	DUF2905 domain-containing protein	-
NWAT_RS01985 (Nwat_0402)	-	418052..419005 (+)	954	WP_013219479.1	Nudix family hydrolase	-
NWAT_RS01990 (Nwat_0403)	yacG	419013..419201 (-)	189	WP_013219480.1	DNA gyrase inhibitor YacG	-
NWAT_RS01995 (Nwat_0404)	coaE	419207..419857 (-)	651	WP_013219481.1	dephospho-CoA kinase	-
NWAT_RS02000 (Nwat_0405)	pilD	419866..420732 (-)	867	WP_013219482.1	A24 family peptidase	Machinery gene
NWAT_RS02005 (Nwat_0406)	pilC	420752..421972 (-)	1221	WP_013219483.1	type II secretion system F family protein	Machinery gene
NWAT_RS02010 (Nwat_0407)	pilB	421977..423692 (-)	1716	WP_041350397.1	type IV-A pilus assembly ATPase PilB	Machinery gene
NWAT_RS17950	lptM	423936..424034 (+)	99	WP_157679984.1	LPS translocon maturation chaperone LptM	-
NWAT_RS02015 (Nwat_0408)	lysA	424038..425288 (+)	1251	WP_198342191.1	diaminopimelate decarboxylase	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 32478.48 Da
• Isoelectric Point: 7.0814

>NTDB_id=37762 NWAT_RS02000 WP_013219482.1 419866..420732(-) (pilD) [Nitrosococcus watsonii C-113]
MEAIVFFESYPAVFMASVFLLGLAVGSFLNVVIYRLPLMMERQWEDQCAELHGKPLPEREPLTLSLPRSHCPACRHKIGA
WENIPLFSYLLLRGHCRYCKAPISLRYPLVELLTGILSIAVAWRFGVSWETMAALLLSYALITLTFIDFDHQLLPDNITL
PFLWFGLGLGLFEVFTDARTSLIGAITGYLSLWLVYHLFRLLTKKEGMGYGDFKLLALLGAWLGWTMLPAIILLSSIVGA
LLGTAWLYLSSQHRETPIPFGPYLAAAGWLALMWGQDINQFYLSLTGR

Nucleotide

• Length: 867 bp

>NTDB_id=37762 NWAT_RS02000 WP_013219482.1 419866..420732(-) (pilD) [Nitrosococcus watsonii C-113]
ATGGAAGCTATTGTGTTTTTCGAGAGTTATCCCGCTGTCTTTATGGCATCGGTTTTTTTGCTGGGCTTAGCCGTCGGCAG
TTTCCTCAATGTCGTTATTTACCGTCTTCCTCTCATGATGGAAAGACAATGGGAAGATCAGTGCGCAGAACTACACGGCA
AGCCACTGCCGGAGCGGGAACCGTTAACGCTATCTCTCCCCCGCTCCCACTGCCCTGCCTGCCGCCACAAAATCGGGGCC
TGGGAGAACATACCTTTATTCAGCTACCTCTTGCTTAGGGGACACTGCCGTTACTGCAAGGCGCCTATCTCTCTCCGCTA
TCCTCTGGTAGAACTACTAACCGGCATCCTTTCTATTGCCGTAGCTTGGCGCTTTGGCGTCAGTTGGGAAACAATGGCGG
CATTGCTCTTAAGCTATGCGCTGATTACGCTCACCTTTATTGATTTCGATCATCAACTGCTGCCCGACAATATTACGCTC
CCTTTTCTTTGGTTTGGTCTAGGGCTAGGTTTATTTGAAGTGTTCACGGATGCCCGAACCAGTCTTATTGGTGCGATAAC
CGGTTATCTTTCCCTATGGCTGGTTTACCATCTGTTTCGCCTCCTGACCAAAAAAGAGGGCATGGGGTACGGCGATTTTA
AACTTTTAGCACTCCTCGGCGCTTGGCTAGGCTGGACGATGCTGCCTGCCATTATTTTGCTTTCCTCGATCGTAGGCGCC
CTGCTAGGAACCGCATGGCTTTATCTTTCCAGCCAGCACCGGGAGACTCCTATCCCATTCGGCCCTTACCTCGCCGCCGC
AGGCTGGCTTGCCTTGATGTGGGGGCAGGATATCAACCAATTTTATCTTTCCCTCACCGGAAGATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

• Type IV leader peptidase family (Peptidase_A24) (136-243)

3D structure

• AlphaFold DB: D8KA57

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	53.684	98.958	0.531
pilD	Vibrio cholerae strain A1552	54.255	97.917	0.531
pilD	Acinetobacter nosocomialis M2	49.826	99.653	0.497
pilD	Acinetobacter baumannii D1279779	49.129	99.653	0.49
pilD	Neisseria gonorrhoeae MS11	50.575	90.625	0.458