Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   FGL86_RS15135 Genome accession   NZ_CP042382
Coordinates   3199534..3200763 (+) Length   409 a.a.
NCBI ID   WP_147185474.1    Uniprot ID   A0A5B8SZQ2
Organism   Pistricoccus aurantiacus strain CBA4606     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3194534..3205763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL86_RS15095 (FGL86_15095) - 3195048..3195272 (+) 225 WP_147185463.1 hypothetical protein -
  FGL86_RS15100 (FGL86_15100) - 3195294..3195569 (-) 276 WP_147185465.1 XRE family transcriptional regulator -
  FGL86_RS15105 (FGL86_15105) - 3195610..3195909 (-) 300 WP_147185467.1 helix-turn-helix domain-containing protein -
  FGL86_RS15110 (FGL86_15110) - 3195902..3196261 (-) 360 WP_222433760.1 type II toxin-antitoxin system RelE/ParE family toxin -
  FGL86_RS15115 (FGL86_15115) - 3196268..3196465 (-) 198 WP_147185469.1 transposase -
  FGL86_RS15120 (FGL86_15120) - 3196465..3196857 (-) 393 WP_147185471.1 type II toxin-antitoxin system VapC family toxin -
  FGL86_RS15125 (FGL86_15125) - 3196930..3197490 (-) 561 WP_342780048.1 pilin -
  FGL86_RS15130 (FGL86_15130) pilB 3197657..3199411 (+) 1755 WP_147185472.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FGL86_RS15135 (FGL86_15135) pilC 3199534..3200763 (+) 1230 WP_147185474.1 type II secretion system F family protein Machinery gene
  FGL86_RS15140 (FGL86_15140) - 3200775..3201650 (+) 876 WP_147185476.1 A24 family peptidase -
  FGL86_RS15145 (FGL86_15145) coaE 3201653..3202255 (+) 603 WP_147186233.1 dephospho-CoA kinase -
  FGL86_RS15150 (FGL86_15150) - 3202357..3202635 (+) 279 WP_147185478.1 CopG family ribbon-helix-helix protein -
  FGL86_RS15155 (FGL86_15155) - 3202662..3202913 (+) 252 WP_246131811.1 type II toxin-antitoxin system RelE/ParE family toxin -
  FGL86_RS15160 (FGL86_15160) yacG 3202999..3203238 (+) 240 WP_147185482.1 DNA gyrase inhibitor YacG -
  FGL86_RS15165 (FGL86_15165) - 3203250..3204134 (+) 885 WP_147185484.1 DNA replication terminus site-binding protein -
  FGL86_RS15170 (FGL86_15170) nqrM 3204152..3204412 (-) 261 WP_147185486.1 (Na+)-NQR maturation NqrM -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44421.91 Da        Isoelectric Point: 10.1536

>NTDB_id=377532 FGL86_RS15135 WP_147185474.1 3199534..3200763(+) (pilC) [Pistricoccus aurantiacus strain CBA4606]
MATRQTKASKEAKLYRWQWTGKGPAGRKVSGEVIAGARQEVVDELSGQNVMVKRVRKKGGLSGGGRVKARDVMLFARQMA
TMIRAGVPILQAFSVVAESLKKPAMVDLVRRMKNDVSAGASFSEALASHPRYFDKLFVSMVAAGEQAGALDNMLERIATY
KEKTEALKARVKKALYYPVAVVLVGIGVTAVLLIKVVPQFESLFSGFGAELPAMTQMTIRMSEFAQHYWLWGLAGVVAVG
IAFFQALKRSSAFRYRVHALALKLPVLGNILDKSAVSQYSRTLAITFAAGVPLVEALNTAAGAVGNNVYERAIHQIREDV
ANGQQLNFAMRLTERFPSLATQMVGIGEESGSLDAMLNRVADYFEEEVDNQVDALTSLLEPFIIVILGVLVGGLVVSMYL
PIFELGSVV

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=377532 FGL86_RS15135 WP_147185474.1 3199534..3200763(+) (pilC) [Pistricoccus aurantiacus strain CBA4606]
ATGGCAACGCGGCAGACGAAGGCGTCGAAAGAAGCGAAGCTCTACCGCTGGCAGTGGACCGGCAAGGGTCCGGCGGGAAG
AAAGGTCAGCGGCGAGGTAATCGCCGGGGCCCGTCAGGAAGTCGTCGATGAGCTTTCCGGCCAGAACGTGATGGTCAAGC
GGGTACGCAAGAAAGGCGGCCTGAGCGGCGGCGGCCGGGTCAAGGCCCGGGACGTGATGCTTTTCGCCCGCCAGATGGCC
ACCATGATTCGTGCCGGGGTGCCGATACTGCAGGCCTTCTCCGTGGTGGCGGAAAGCCTCAAGAAGCCCGCCATGGTGGA
TCTGGTGCGGCGCATGAAGAACGACGTCAGCGCCGGCGCCAGTTTCTCCGAGGCCCTGGCAAGCCATCCCAGGTATTTCG
ACAAGCTGTTCGTCAGCATGGTGGCCGCCGGGGAACAGGCCGGCGCCCTGGACAACATGCTGGAGCGTATCGCCACCTAC
AAGGAAAAGACCGAGGCGCTGAAGGCGCGGGTCAAGAAGGCGCTCTATTACCCGGTGGCGGTGGTTCTCGTGGGCATCGG
CGTGACCGCGGTGCTGTTGATCAAGGTGGTGCCCCAGTTCGAAAGCCTGTTTTCCGGCTTCGGCGCGGAGCTGCCCGCCA
TGACCCAGATGACGATTCGCATGTCGGAATTCGCCCAGCACTATTGGCTGTGGGGCCTTGCCGGCGTGGTCGCGGTGGGT
ATCGCTTTCTTCCAGGCCTTGAAGCGCTCCTCGGCCTTTCGCTATCGGGTGCATGCGCTGGCGCTGAAGCTGCCGGTGCT
GGGCAACATTCTCGACAAGTCCGCGGTGTCCCAGTATTCCCGCACCCTGGCCATCACCTTCGCCGCCGGCGTGCCGCTGG
TGGAGGCGCTGAACACCGCCGCCGGCGCCGTGGGCAACAACGTCTACGAGCGGGCCATTCATCAGATTCGCGAAGACGTG
GCCAACGGCCAGCAGCTCAACTTCGCCATGCGCCTGACGGAGCGCTTTCCCTCCCTGGCGACCCAGATGGTCGGCATCGG
CGAGGAATCCGGTTCCCTGGACGCCATGCTCAACCGGGTGGCGGACTACTTCGAGGAAGAGGTGGACAACCAGGTGGACG
CCCTGACCTCGCTGCTGGAACCCTTTATCATCGTCATATTGGGCGTACTGGTGGGCGGCCTGGTGGTCTCCATGTACCTG
CCGATCTTCGAGCTGGGCAGCGTGGTGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(280-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8SZQ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

52.723

98.778

0.521
  pilC Acinetobacter baumannii D1279779

52.141

97.066

0.506
  pilC Acinetobacter baylyi ADP1

51.005

97.311

0.496
  pilC Legionella pneumophila strain ERS1305867

49.875

97.555

0.487
  pilC Vibrio cholerae strain A1552

44.963

99.511

0.447
  pilC Vibrio campbellii strain DS40M4

44.363

99.756

0.443
  pilG Neisseria gonorrhoeae MS11

42.289

98.289

0.416
  pilG Neisseria meningitidis 44/76-A

42.394

98.044

0.416

Multiple sequence alignment