Detailed information NTG0377531

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: FGL86_RS15130
• Genome accession: NZ_CP042382
• Coordinates: 3197657..3199411 (+)
• Length: 584 a.a.
• NCBI ID: WP_147185472.1
• Uniprot ID: A0A5B8ST43
• Organism: Pistricoccus aurantiacus strain CBA4606
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3192657..3204411

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FGL86_RS15085 (FGL86_15085)	-	3192903..3194099 (+)	1197	WP_147185459.1	FliC/FljB family flagellin	-
FGL86_RS15090 (FGL86_15090)	-	3194192..3194851 (+)	660	WP_147185461.1	hypothetical protein	-
FGL86_RS15095 (FGL86_15095)	-	3195048..3195272 (+)	225	WP_147185463.1	hypothetical protein	-
FGL86_RS15100 (FGL86_15100)	-	3195294..3195569 (-)	276	WP_147185465.1	XRE family transcriptional regulator	-
FGL86_RS15105 (FGL86_15105)	-	3195610..3195909 (-)	300	WP_147185467.1	helix-turn-helix domain-containing protein	-
FGL86_RS15110 (FGL86_15110)	-	3195902..3196261 (-)	360	WP_222433760.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FGL86_RS15115 (FGL86_15115)	-	3196268..3196465 (-)	198	WP_147185469.1	transposase	-
FGL86_RS15120 (FGL86_15120)	-	3196465..3196857 (-)	393	WP_147185471.1	type II toxin-antitoxin system VapC family toxin	-
FGL86_RS15125 (FGL86_15125)	-	3196930..3197490 (-)	561	WP_342780048.1	pilin	-
FGL86_RS15130 (FGL86_15130)	pilB	3197657..3199411 (+)	1755	WP_147185472.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FGL86_RS15135 (FGL86_15135)	pilC	3199534..3200763 (+)	1230	WP_147185474.1	type II secretion system F family protein	Machinery gene
FGL86_RS15140 (FGL86_15140)	-	3200775..3201650 (+)	876	WP_147185476.1	A24 family peptidase	-
FGL86_RS15145 (FGL86_15145)	coaE	3201653..3202255 (+)	603	WP_147186233.1	dephospho-CoA kinase	-
FGL86_RS15150 (FGL86_15150)	-	3202357..3202635 (+)	279	WP_147185478.1	CopG family ribbon-helix-helix protein	-
FGL86_RS15155 (FGL86_15155)	-	3202662..3202913 (+)	252	WP_246131811.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FGL86_RS15160 (FGL86_15160)	yacG	3202999..3203238 (+)	240	WP_147185482.1	DNA gyrase inhibitor YacG	-
FGL86_RS15165 (FGL86_15165)	-	3203250..3204134 (+)	885	WP_147185484.1	DNA replication terminus site-binding protein	-

Sequence

Protein

• Length: 584 a.a.
• Molecular weight: 63543.43 Da
• Isoelectric Point: 4.8253

>NTDB_id=377531 FGL86_RS15130 WP_147185472.1 3197657..3199411(+) (pilB) [Pistricoccus aurantiacus strain CBA4606]
MPFSPAPPSDAAPSITLRGLGRRLVDQGLLTETLAAQAVEGAKAAGTSLMHYVIEQELASPEETACAAAWEYGMPLIDLE
ALRISALPAADRMPEQLLRRHCMLPVACNEYRLTVAVAYPSVLSHLDELGVATGLTVEGVLAPANQLQSMLDRYLQQHEQ
AQLSDQLGSGDSSLEDIDFEGSGNQEAEEDQAVTAAESDAPIVRFVNKVLLDAIQRGASDIHFEPYETSYRIRLRIDGIL
LETARPPFGQRNRIAARLKVISRLDISERRLPQDGAIKLRVSKTRSIDFRVSTLPTVYGEKIVLRILDPASAQMGIDALG
FTQSQRALYEQALSEPQGMVLVTGPTGSGKTVTLYTGLNMLNTDERNISTAEDPVEIKVPGINQVNVLPRIGLTFASALR
AFLRQDPDVVMVGEIRDLETAEIAVKASQTGHLVLSTLHTNSAAETLTRMINMGVQAFNIASSVSLIIAQRLARRLCPYC
KTPTEIPHQALIEAGFSEEEIGDATIYRPVGCRRCTLGYKGRVGIYEVVPISDAMSQLIMQQGNSLELAQQARDEGHPDL
RSSALAKAMEGLTSLEEVHRVTTD

Nucleotide

• Length: 1755 bp

>NTDB_id=377531 FGL86_RS15130 WP_147185472.1 3197657..3199411(+) (pilB) [Pistricoccus aurantiacus strain CBA4606]
ATGCCCTTCTCACCCGCCCCGCCTTCCGACGCCGCCCCGAGCATCACGCTACGGGGGCTGGGGCGGCGCCTGGTCGATCA
GGGGTTGCTGACGGAAACGCTGGCGGCACAGGCAGTGGAAGGCGCCAAGGCGGCGGGCACCTCGCTGATGCACTATGTGA
TTGAGCAGGAATTGGCCAGCCCGGAGGAAACCGCCTGCGCTGCGGCCTGGGAATACGGCATGCCGCTGATCGACCTGGAA
GCGCTGCGAATTTCCGCTCTGCCCGCGGCGGATCGCATGCCGGAACAGCTGCTGCGCCGCCACTGCATGCTGCCGGTGGC
CTGCAACGAGTATCGGCTGACGGTGGCGGTGGCCTACCCGTCGGTACTCAGCCATCTGGACGAGCTGGGAGTGGCCACCG
GGCTGACGGTGGAAGGAGTGCTGGCCCCGGCGAATCAGCTGCAGAGCATGCTCGATCGCTATCTGCAGCAGCACGAGCAG
GCCCAGCTCAGCGATCAGCTTGGCAGCGGGGATTCCAGCCTCGAGGATATCGACTTCGAAGGCAGCGGCAACCAGGAGGC
GGAAGAGGATCAGGCGGTCACCGCCGCCGAGAGCGACGCACCCATCGTGCGCTTCGTCAACAAGGTACTGCTGGACGCCA
TTCAGCGCGGCGCCTCGGATATCCATTTCGAGCCCTACGAGACCAGCTACCGCATCCGCCTGCGGATCGACGGCATTCTG
CTGGAAACCGCCAGGCCGCCCTTCGGCCAGCGCAACCGTATCGCCGCCCGCCTGAAGGTCATCTCCCGGCTGGATATCTC
CGAGCGCCGCCTGCCCCAGGACGGCGCGATCAAGCTGCGGGTGTCCAAGACCCGCTCCATCGATTTCCGCGTCAGCACCC
TGCCTACGGTGTACGGCGAGAAGATCGTGCTGCGGATACTCGATCCGGCCTCCGCCCAGATGGGCATCGACGCCCTGGGC
TTCACCCAATCCCAGCGTGCGCTCTACGAGCAGGCGCTTTCCGAGCCCCAGGGCATGGTGCTGGTCACCGGCCCCACCGG
CAGCGGCAAGACGGTGACGCTGTATACCGGCCTCAATATGCTCAACACGGACGAGCGCAATATTTCCACCGCGGAAGACC
CGGTGGAGATCAAGGTACCGGGCATCAATCAGGTCAACGTGCTGCCGCGCATCGGCCTGACCTTCGCCAGCGCCCTGCGC
GCCTTTTTGCGCCAGGACCCGGACGTGGTGATGGTGGGCGAGATCCGCGATCTGGAAACCGCGGAAATCGCCGTCAAGGC
GTCCCAGACCGGCCACCTGGTGCTTTCCACCCTGCACACCAACTCCGCCGCGGAAACCCTGACCCGCATGATCAACATGG
GGGTTCAGGCCTTCAATATCGCCAGCTCCGTGTCGCTGATCATCGCCCAGCGCCTGGCCCGGCGGCTGTGCCCCTACTGC
AAGACGCCCACGGAGATTCCGCACCAGGCGCTGATCGAGGCGGGCTTCAGCGAGGAGGAGATCGGCGATGCGACGATCTA
TCGCCCGGTGGGCTGTCGCCGCTGCACCCTGGGCTACAAGGGCCGGGTGGGCATCTACGAGGTGGTGCCGATCAGTGATG
CCATGAGCCAGCTGATCATGCAGCAGGGGAATTCCCTGGAACTGGCGCAGCAGGCCCGCGATGAAGGTCACCCGGATCTG
CGCAGCAGTGCCTTGGCCAAGGCCATGGAGGGGTTGACCAGTCTCGAGGAAGTGCATCGAGTCACTACCGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (208-475)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (54-159)

3D structure

• AlphaFold DB: A0A5B8ST43

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Legionella pneumophila strain ERS1305867	53.158	97.603	0.519
pilB	Acinetobacter baumannii D1279779	51.736	98.63	0.51
pilB	Acinetobacter baylyi ADP1	51.232	97.26	0.498
pilB	Vibrio cholerae strain A1552	49.293	96.918	0.478
pilB	Vibrio campbellii strain DS40M4	48.772	97.603	0.476
pilB	Vibrio parahaemolyticus RIMD 2210633	49.378	96.404	0.476
pilF	Neisseria gonorrhoeae MS11	47.193	97.603	0.461
pilB/pilB1	Synechocystis sp. PCC 6803	36.721	100	0.384
pilF	Thermus thermophilus HB27	39.184	96.575	0.378
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.869	96.918	0.377