Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   HBA47_RS09485 Genome accession   NZ_CP050136
Coordinates   1806989..1807417 (-) Length   142 a.a.
NCBI ID   WP_154700048.1    Uniprot ID   -
Organism   Kingella kingae strain ATCC 23332     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1801989..1812417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBA47_RS09450 (HBA47_09490) - 1802393..1803238 (+) 846 WP_019390091.1 mechanosensitive ion channel family protein -
  HBA47_RS09455 (HBA47_09495) - 1803500..1803658 (+) 159 WP_019390092.1 hypothetical protein -
  HBA47_RS09460 (HBA47_09500) - 1803661..1804386 (+) 726 WP_019390093.1 metal ABC transporter ATP-binding protein -
  HBA47_RS09465 (HBA47_09505) - 1804435..1804827 (+) 393 WP_003791724.1 pyrimidine dimer DNA glycosylase/endonuclease V -
  HBA47_RS09470 (HBA47_09510) - 1804865..1805755 (+) 891 WP_019390094.1 metal ABC transporter permease -
  HBA47_RS09480 (HBA47_09520) - 1806201..1806977 (-) 777 WP_019390095.1 fimb protein -
  HBA47_RS09485 (HBA47_09525) pilE 1806989..1807417 (-) 429 WP_154700048.1 pilin Machinery gene
  HBA47_RS09490 (HBA47_09530) pilE 1807562..1808032 (-) 471 WP_154700047.1 pilin Machinery gene
  HBA47_RS09495 (HBA47_09535) recJ 1808389..1810101 (+) 1713 WP_154700046.1 single-stranded-DNA-specific exonuclease RecJ -
  HBA47_RS09500 (HBA47_09540) - 1810213..1810947 (+) 735 WP_003791735.1 histidine phosphatase family protein -
  HBA47_RS09505 (HBA47_09545) - 1810986..1811531 (+) 546 WP_154700045.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14826.96 Da        Isoelectric Point: 7.1323

>NTDB_id=377076 HBA47_RS09485 WP_154700048.1 1806989..1807417(-) (pilE) [Kingella kingae strain ATCC 23332]
METKMQGFTLIELMIVIAIVGILAALALPTYQDFSVRSRVAEGLSLAGGAKTSVAEYYASHNQFPANNVTAGLATANQIK
GSSVDSLTVVDGVITVVFNNKVAAAGKNSLVLTPSVAKEGSITWDCHGGSLDGRLRPSECRN

Nucleotide


Download         Length: 429 bp        

>NTDB_id=377076 HBA47_RS09485 WP_154700048.1 1806989..1807417(-) (pilE) [Kingella kingae strain ATCC 23332]
ATGGAAACTAAAATGCAAGGTTTTACCTTAATTGAATTGATGATTGTTATTGCTATTGTGGGTATTTTAGCTGCGCTGGC
ATTGCCAACGTATCAGGATTTTTCAGTACGCTCGCGTGTGGCAGAAGGATTGAGTTTGGCTGGTGGTGCAAAAACATCGG
TGGCTGAATATTATGCTTCGCATAACCAATTTCCAGCCAATAATGTAACGGCAGGTTTGGCTACAGCCAACCAAATTAAG
GGCAGTTCGGTGGATTCGCTTACAGTTGTTGATGGTGTGATTACGGTGGTATTTAATAATAAAGTGGCGGCAGCAGGTAA
AAATTCGCTGGTTTTAACGCCTAGTGTGGCGAAAGAAGGCTCTATTACTTGGGATTGCCACGGTGGTTCATTGGATGGCA
GATTGCGTCCATCGGAATGCCGTAATTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)

Pilin (bacterial filament) (Pilin)

(36-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

40.244

100

0.465
  pilA Ralstonia pseudosolanacearum GMI1000

40

100

0.465
  pilE Neisseria gonorrhoeae strain FA1090

39.877

100

0.458
  pilA/pilA1 Eikenella corrodens VA1

40.645

100

0.444
  pilA2 Legionella pneumophila str. Paris

44.604

97.887

0.437
  pilA2 Legionella pneumophila strain ERS1305867

43.885

97.887

0.43
  comP Acinetobacter baylyi ADP1

39.61

100

0.43
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.806

100

0.373