Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   FRC97_RS13295 Genome accession   NZ_CP042323
Coordinates   2852511..2853728 (-) Length   405 a.a.
NCBI ID   WP_017438049.1    Uniprot ID   -
Organism   Paracidovorax citrulli strain NWB SC196     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2847511..2858728
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FRC97_RS13265 (FRC97_13280) - 2848522..2849394 (+) 873 WP_011796729.1 ATP-binding protein -
  FRC97_RS13270 (FRC97_13285) - 2849387..2849842 (+) 456 WP_011796728.1 NUDIX domain-containing protein -
  FRC97_RS13275 (FRC97_13290) - 2849900..2850154 (-) 255 WP_011796727.1 DNA gyrase inhibitor YacG -
  FRC97_RS13280 (FRC97_13295) zapD 2850181..2850936 (-) 756 WP_041828126.1 cell division protein ZapD -
  FRC97_RS13285 (FRC97_13300) coaE 2851001..2851627 (-) 627 WP_011796725.1 dephospho-CoA kinase -
  FRC97_RS13290 (FRC97_13305) - 2851624..2852511 (-) 888 WP_017438048.1 A24 family peptidase -
  FRC97_RS13295 (FRC97_13310) pilC 2852511..2853728 (-) 1218 WP_017438049.1 type II secretion system F family protein Machinery gene
  FRC97_RS13300 (FRC97_13315) pilB 2853760..2855496 (-) 1737 WP_011796722.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FRC97_RS13305 (FRC97_13320) - 2855805..2856737 (-) 933 WP_017438050.1 LysR substrate-binding domain-containing protein -
  FRC97_RS13310 (FRC97_13325) - 2856837..2857820 (+) 984 WP_011796720.1 tripartite tricarboxylate transporter substrate binding protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44572.45 Da        Isoelectric Point: 8.9302

>NTDB_id=377069 FRC97_RS13295 WP_017438049.1 2852511..2853728(-) (pilC) [Paracidovorax citrulli strain NWB SC196]
MATAASRDIKDFVFEWEGKDRHGKVVRGEVRALGENQVQATLRRQGVLPTKIKKRRMRSGKKIKPKDIALFTRQMATMMK
AGVPLLQAFDIVGRGNTNASVTKLLNDIRADVETGTSLNAAFRKYPMYFDSLYCNLVEAGEAAGILEALLDRLALYMEKT
EAIKSKIRSALMYPTAVIIVAFVVVTVIMIFVIPAFKEVFTSFGADLPAPTLFVMGVSEIFVQWWWLIFGVLGGGAYFFM
QAWRRNEKMQMFMDRLLLRVPVFGALIDKSCVARWTRTLSTMFAAGVPLVEALDSVGGASGNSVYAMATERIQQEVSTGT
SLTAAMGNANVFPSMVIQMCAIGEESGSIDHMLGKAADFYEEEVDEMVAGLSSLMEPIIIVFLGTIIGGIVVSMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=377069 FRC97_RS13295 WP_017438049.1 2852511..2853728(-) (pilC) [Paracidovorax citrulli strain NWB SC196]
ATGGCAACCGCTGCATCGAGGGACATCAAGGATTTCGTTTTCGAGTGGGAAGGCAAGGACCGCCACGGCAAGGTCGTGCG
CGGCGAGGTCCGGGCCCTGGGCGAGAACCAGGTCCAGGCGACGCTGCGGCGGCAGGGGGTGCTGCCCACCAAGATCAAGA
AGCGCCGGATGCGCAGCGGCAAGAAGATCAAGCCCAAGGACATCGCGCTCTTCACGCGGCAGATGGCCACGATGATGAAG
GCCGGGGTCCCCCTGCTGCAGGCCTTCGACATCGTGGGCCGGGGCAACACCAACGCCTCGGTCACCAAGCTGCTCAACGA
CATCCGCGCCGACGTGGAAACCGGTACTTCGCTGAACGCGGCATTCCGCAAGTACCCGATGTACTTCGACAGCCTGTACT
GCAACCTGGTGGAAGCCGGGGAGGCGGCGGGTATCCTGGAAGCACTGCTCGACCGGCTCGCGCTCTACATGGAGAAGACC
GAGGCCATCAAGTCGAAGATCCGCTCGGCGCTGATGTATCCGACGGCCGTGATCATCGTGGCCTTCGTCGTGGTCACCGT
CATCATGATCTTCGTGATCCCCGCGTTCAAGGAGGTGTTCACCTCGTTCGGTGCCGACCTGCCGGCGCCCACCCTGTTCG
TGATGGGCGTCAGCGAGATCTTCGTCCAATGGTGGTGGCTGATCTTCGGCGTGCTGGGCGGCGGCGCATACTTCTTCATG
CAGGCATGGCGGCGCAACGAGAAGATGCAGATGTTCATGGACCGGCTGCTGCTGCGCGTGCCGGTCTTCGGCGCCCTGAT
CGACAAGTCCTGCGTGGCGCGCTGGACCCGCACCCTCTCCACGATGTTCGCGGCGGGCGTTCCGCTGGTCGAGGCGCTGG
ACTCGGTGGGAGGGGCCTCGGGCAACTCGGTCTATGCGATGGCGACCGAAAGGATCCAGCAGGAAGTCTCCACCGGCACC
AGCCTGACGGCCGCGATGGGCAACGCGAACGTGTTCCCGTCCATGGTGATCCAGATGTGCGCCATCGGCGAGGAGTCGGG
CTCGATCGACCACATGCTGGGCAAGGCGGCCGACTTCTACGAGGAGGAGGTCGATGAAATGGTCGCGGGGCTGTCGAGCC
TCATGGAGCCGATCATCATCGTGTTCCTGGGCACCATCATCGGCGGCATCGTGGTGTCGATGTACCTGCCCATCTTCAAG
CTGGGACAGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.208

99.753

0.541
  pilG Neisseria gonorrhoeae MS11

51.378

98.519

0.506
  pilG Neisseria meningitidis 44/76-A

51.128

98.519

0.504
  pilC Legionella pneumophila strain ERS1305867

48.744

98.272

0.479
  pilC Acinetobacter baylyi ADP1

48.744

98.272

0.479
  pilC Acinetobacter baumannii D1279779

47.103

98.025

0.462
  pilC Vibrio cholerae strain A1552

40.302

98.025

0.395
  pilC Vibrio campbellii strain DS40M4

39.152

99.012

0.388
  pilC Thermus thermophilus HB27

38.614

99.753

0.385

Multiple sequence alignment