Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   HBA48_RS09535 Genome accession   NZ_CP050135
Coordinates   1840437..1840865 (-) Length   142 a.a.
NCBI ID   WP_019390096.1    Uniprot ID   B6SE74
Organism   Kingella kingae strain ATCC 23331     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1835437..1845865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBA48_RS09500 (HBA48_09540) - 1835950..1836795 (+) 846 WP_003791720.1 mechanosensitive ion channel family protein -
  HBA48_RS09505 (HBA48_09545) - 1836950..1837108 (+) 159 WP_164538012.1 hypothetical protein -
  HBA48_RS09510 (HBA48_09550) - 1837111..1837836 (+) 726 WP_038302563.1 metal ABC transporter ATP-binding protein -
  HBA48_RS09515 (HBA48_09555) - 1837885..1838277 (+) 393 WP_038302564.1 pyrimidine dimer DNA glycosylase/endonuclease V -
  HBA48_RS09520 (HBA48_09560) - 1838313..1839203 (+) 891 WP_003788327.1 metal ABC transporter permease -
  HBA48_RS09530 (HBA48_09570) - 1839649..1840425 (-) 777 WP_038302565.1 fimb protein -
  HBA48_RS09535 (HBA48_09575) pilE 1840437..1840865 (-) 429 WP_019390096.1 pilin Machinery gene
  HBA48_RS09540 (HBA48_09580) pilE 1841012..1841485 (-) 474 WP_164538010.1 pilin Machinery gene
  HBA48_RS09545 (HBA48_09585) recJ 1841845..1843557 (+) 1713 WP_038310302.1 single-stranded-DNA-specific exonuclease RecJ -
  HBA48_RS09550 (HBA48_09590) - 1843774..1844508 (+) 735 WP_003791735.1 histidine phosphatase family protein -
  HBA48_RS09555 (HBA48_09595) - 1844548..1845093 (+) 546 WP_038310304.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14865.01 Da        Isoelectric Point: 7.8822

>NTDB_id=377015 HBA48_RS09535 WP_019390096.1 1840437..1840865(-) (pilE) [Kingella kingae strain ATCC 23331]
METKMQGFTLIELMIVIAIVGILAALALPTYQDFSVRSRVAEGLSLAGGAKTSVAEYYASHNQFPANNVTAGLATANQIK
GSSVHSLTVTDGVITVVFNNKVAAAGKNSLVLTPSVAKEGSITWDCHGGTLDGRLRPSECRN

Nucleotide


Download         Length: 429 bp        

>NTDB_id=377015 HBA48_RS09535 WP_019390096.1 1840437..1840865(-) (pilE) [Kingella kingae strain ATCC 23331]
ATGGAAACTAAAATGCAAGGTTTTACCTTAATTGAATTGATGATTGTTATTGCTATTGTGGGTATTTTAGCTGCGCTGGC
ATTGCCAACGTATCAGGATTTTTCAGTACGCTCGCGTGTGGCAGAAGGATTGAGTTTGGCTGGTGGTGCAAAAACATCGG
TGGCAGAATACTATGCTTCGCATAACCAATTTCCAGCCAATAATGTAACGGCAGGTTTGGCTACAGCCAACCAAATTAAG
GGCAGTTCGGTGCATTCGCTTACGGTAACAGATGGTGTGATTACGGTGGTATTTAATAATAAAGTGGCGGCAGCAGGTAA
AAATTCGCTGGTTTTAACGCCTAGTGTGGCGAAAGAAGGCTCTATTACTTGGGATTGCCACGGTGGTACATTGGACGGCA
GATTGCGTCCATCGGAATGCCGTAATTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)

Pilin (bacterial filament) (Pilin)

(36-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B6SE74

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

40.244

100

0.465
  pilA Ralstonia pseudosolanacearum GMI1000

40

100

0.465
  pilE Neisseria gonorrhoeae strain FA1090

39.877

100

0.458
  pilA/pilA1 Eikenella corrodens VA1

41.935

100

0.458
  pilA2 Legionella pneumophila str. Paris

45.324

97.887

0.444
  comP Acinetobacter baylyi ADP1

40.26

100

0.437
  pilA2 Legionella pneumophila strain ERS1305867

44.604

97.887

0.437
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.806

100

0.373