Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FPZ22_RS09880 Genome accession   NZ_CP042218
Coordinates   2146586..2148310 (-) Length   574 a.a.
NCBI ID   WP_144892578.1    Uniprot ID   A0A518N5G1
Organism   Luteimonas granuli strain Gr-4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2141586..2153310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FPZ22_RS09860 (FPZ22_09860) coaE 2141967..2142593 (-) 627 WP_144892570.1 dephospho-CoA kinase -
  FPZ22_RS09865 (FPZ22_09865) - 2142694..2144160 (+) 1467 WP_144892572.1 Fic family protein -
  FPZ22_RS09870 (FPZ22_09870) pilD 2144295..2145155 (-) 861 WP_144892574.1 A24 family peptidase Machinery gene
  FPZ22_RS09875 (FPZ22_09875) pilC 2145159..2146313 (-) 1155 WP_246121038.1 type II secretion system F family protein Machinery gene
  FPZ22_RS09880 (FPZ22_09880) pilB 2146586..2148310 (-) 1725 WP_144892578.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FPZ22_RS09885 (FPZ22_09885) - 2148350..2149852 (-) 1503 WP_144892580.1 hypothetical protein -
  FPZ22_RS09890 (FPZ22_09890) - 2149849..2150463 (-) 615 WP_144892583.1 hypothetical protein -
  FPZ22_RS09895 (FPZ22_09895) - 2150479..2152239 (-) 1761 WP_144892585.1 phosphoethanolamine transferase -
  FPZ22_RS09900 (FPZ22_09900) pilA2 2152327..2152770 (-) 444 WP_144892587.1 pilin Machinery gene

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62493.64 Da        Isoelectric Point: 5.2265

>NTDB_id=376063 FPZ22_RS09880 WP_144892578.1 2146586..2148310(-) (pilB) [Luteimonas granuli strain Gr-4]
MSAAPMSSLAGITGIARRLVMDGVLDEATAREALDKATAERKPVASYLREYKLITSAQLTAANSIEFGMPVFDPQTMDAS
QSAIKLVSDELLGKHAVLPLFKRGGRLFVGIADPTNTHALDEIKFHTNLAVEPILVDEDRIRRCMELWQDAGDSFGEELG
DSEGLDTLDISGGDEDGADSGVDAKGDDTPVVKFVNKVLIDAIKRGASDIHFEPYENDYRVRLRIDGILKQSAKMPVKLN
QRITARLKVMAQLDIAEKRVPQDGRIKLNLSRTRQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQDLFLD
AIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSRCKQPQELPDNAL
LAEGFTSDEVHAGLTIYEAVGCDECTGGYKGRLGVYQVMPMSEAIQEIVLKGGNALDIAEAARASGVRDLRMSALLKVKN
GLTSLAEINRITVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=376063 FPZ22_RS09880 WP_144892578.1 2146586..2148310(-) (pilB) [Luteimonas granuli strain Gr-4]
ATGAGTGCAGCACCCATGAGCAGTCTTGCCGGCATCACCGGTATCGCGCGCCGCTTGGTCATGGACGGGGTCCTCGACGA
GGCCACTGCCCGCGAAGCACTTGATAAAGCTACGGCCGAACGCAAGCCCGTCGCGTCGTATCTGCGCGAATACAAGCTGA
TCACCTCGGCCCAGCTGACGGCCGCCAACTCGATCGAGTTCGGCATGCCGGTGTTCGACCCGCAGACGATGGACGCCTCG
CAGAGCGCCATCAAGCTGGTCAGCGACGAGCTGCTGGGCAAGCACGCCGTCCTGCCGCTGTTCAAGCGCGGCGGCCGGCT
GTTCGTCGGCATCGCCGATCCGACCAACACCCATGCCCTGGACGAGATCAAGTTCCACACCAACCTCGCGGTCGAGCCGA
TCCTGGTGGACGAGGACAGGATCCGCCGCTGCATGGAGCTCTGGCAGGACGCAGGCGACTCCTTCGGCGAGGAGCTGGGC
GACAGCGAGGGCCTGGACACCCTCGACATCTCCGGCGGCGACGAGGACGGCGCCGACTCGGGGGTCGACGCCAAGGGCGA
CGACACCCCGGTGGTCAAGTTCGTCAACAAGGTGCTGATCGACGCCATCAAGCGGGGCGCGTCCGACATCCACTTCGAAC
CCTACGAGAACGACTACCGGGTCCGCCTGCGCATCGACGGCATCCTGAAGCAGTCGGCGAAGATGCCGGTCAAGCTCAAC
CAGCGCATCACCGCCCGCCTCAAGGTCATGGCCCAGCTGGACATCGCCGAGAAGCGCGTGCCCCAGGACGGCCGCATCAA
GCTCAACCTCAGCCGGACCAGGCAGATCGACTTCCGCGTCAGCACCCTGCCCACGCTGTTCGGCGAGAAGGTGGTGCTGC
GCATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAGCAGGACCTGTTCCTGGAC
GCCATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGCTCGGGCAAGACGGTGAGCCTGTACACGGCGCT
GGGCATCCTCAACGACGAGACCCGCAACATCTCGACCGCCGAGGACCCGGTGGAAATCCGCCTGCCGGGCGTGAACCAGG
TGCAGCAGAACGTGAAGCGCGGCATGACCTTCGCGGCCGCGCTGCGCAGCTTCCTGCGCCAGGACCCGGACATCATCATG
GTCGGCGAGATCCGCGACCTGGAGACCGCCGAGATCGCCATCAAGGCGGCCCAGACCGGTCACATGGTCCTCTCGACCCT
GCATACCAACGACGCCCCGCAGACCATCGCCCGCCTGATGAACATGGGCATCGCGCCCTACAACATCACCAGCTCGGTGA
CCCTGGTGATCGCCCAGCGCCTGGCGCGGCGCCTGTGCAGCCGCTGCAAGCAGCCGCAGGAACTCCCGGACAACGCCCTG
CTCGCCGAGGGCTTCACCAGCGACGAGGTCCATGCCGGGCTGACCATCTACGAGGCCGTGGGCTGCGACGAGTGCACCGG
CGGCTACAAGGGCCGACTGGGCGTGTACCAGGTGATGCCGATGAGCGAGGCCATCCAGGAGATCGTGCTCAAGGGCGGCA
ACGCCCTGGACATCGCCGAGGCGGCGCGGGCATCGGGGGTCCGGGATCTGCGCATGTCCGCCCTGCTGAAGGTGAAGAAC
GGGCTGACCAGCCTGGCCGAGATCAACCGCATCACCGTGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-149)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A518N5G1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.282

98.955

0.547
  pilB Acinetobacter baylyi ADP1

53.71

98.606

0.53
  pilB Legionella pneumophila strain ERS1305867

51.767

98.606

0.51
  pilB Vibrio cholerae strain A1552

49.372

97.038

0.479
  pilF Neisseria gonorrhoeae MS11

48.05

98.258

0.472
  pilB Vibrio campbellii strain DS40M4

47.627

99.129

0.472
  pilB Vibrio parahaemolyticus RIMD 2210633

49.716

92.16

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

38.382

100

0.389

Multiple sequence alignment