Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G9W35_RS00320 Genome accession   NZ_CP049845
Coordinates   73226..74611 (+) Length   461 a.a.
NCBI ID   WP_074499817.1    Uniprot ID   -
Organism   Escherichia coli strain UPEC-245U/190328     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 68226..79611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G9W35_RS00295 (G9W35_00290) aroP 68774..70147 (+) 1374 WP_074499820.1 aromatic amino acid transporter AroP -
  G9W35_RS00300 (G9W35_00295) ampE 70190..71044 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  G9W35_RS00305 (G9W35_00300) ampD 71041..71592 (-) 552 WP_074499818.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G9W35_RS00310 (G9W35_00305) nadC 71680..72573 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G9W35_RS00315 (G9W35_00310) pilA 72776..73216 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  G9W35_RS00320 (G9W35_00315) pilB 73226..74611 (+) 1386 WP_074499817.1 type II secretion system protein GspE Machinery gene
  G9W35_RS00325 (G9W35_00320) hofC 74601..75803 (+) 1203 WP_000157235.1 protein transport protein HofC -
  G9W35_RS00330 (G9W35_00325) guaC 75838..76881 (-) 1044 WP_001217338.1 GMP reductase -
  G9W35_RS00335 - 77037..77081 (-) 45 WP_120795372.1 protein YacM -
  G9W35_RS00340 (G9W35_00330) coaE 77106..77726 (+) 621 WP_074499815.1 dephospho-CoA kinase -
  G9W35_RS00345 (G9W35_00335) zapD 77726..78469 (+) 744 WP_001194734.1 cell division protein ZapD -
  G9W35_RS00350 (G9W35_00340) yacG 78479..78676 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  G9W35_RS00355 (G9W35_00345) mutT 78776..79165 (-) 390 WP_000736013.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50601.39 Da        Isoelectric Point: 6.7461

>NTDB_id=375402 G9W35_RS00320 WP_074499817.1 73226..74611(+) (pilB) [Escherichia coli strain UPEC-245U/190328]
MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLKLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=375402 G9W35_RS00320 WP_074499817.1 73226..74611(+) (pilB) [Escherichia coli strain UPEC-245U/190328]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTAAAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGGCTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCAGCAGCAAGGGGAGCCCATCCATATTCCAGTCAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

42.562

100

0.447
  pilB Legionella pneumophila strain ERS1305867

49.74

83.297

0.414
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Acinetobacter baylyi ADP1

39.957

100

0.406
  pilB Vibrio cholerae strain A1552

46.212

85.9

0.397
  pilB Vibrio parahaemolyticus RIMD 2210633

46.632

83.731

0.39
  pilB Haemophilus influenzae 86-028NP

44.703

83.948

0.375
  pilB Acinetobacter baumannii D1279779

43.655

85.466

0.373
  pilB Haemophilus influenzae Rd KW20

44.186

83.948

0.371
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369
  pilF Thermus thermophilus HB27

40.831

88.72

0.362