Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FPL17_RS12435 Genome accession   NZ_CP041970
Coordinates   2712417..2714129 (+) Length   570 a.a.
NCBI ID   WP_159885336.1    Uniprot ID   -
Organism   Acinetobacter dispersus strain NCCP 16014     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2707417..2719129
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FPL17_RS12400 (FPL17_12545) nusA 2708233..2709717 (-) 1485 WP_005191970.1 transcription termination factor NusA -
  FPL17_RS12405 (FPL17_12550) rimP 2709755..2710279 (-) 525 WP_159885334.1 ribosome maturation factor RimP -
  FPL17_RS12425 (FPL17_12570) secG 2710909..2711238 (-) 330 WP_078197608.1 preprotein translocase subunit SecG -
  FPL17_RS12430 (FPL17_12575) tpiA 2711251..2712042 (-) 792 WP_032864570.1 triose-phosphate isomerase -
  FPL17_RS12435 (FPL17_12580) pilB 2712417..2714129 (+) 1713 WP_159885336.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FPL17_RS12440 (FPL17_12585) pilC 2714154..2715380 (+) 1227 WP_078197602.1 type II secretion system F family protein Machinery gene
  FPL17_RS12445 (FPL17_12590) pilD 2715386..2716246 (+) 861 WP_159885338.1 prepilin peptidase Machinery gene
  FPL17_RS12450 (FPL17_12595) coaE 2716248..2716853 (+) 606 WP_159885340.1 dephospho-CoA kinase -
  FPL17_RS12455 (FPL17_12600) - 2716850..2717761 (-) 912 WP_159885342.1 DMT family transporter -
  FPL17_RS12460 (FPL17_12605) rlmB 2717792..2718541 (-) 750 WP_005191946.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  FPL17_RS12465 (FPL17_12610) - 2718710..2719036 (-) 327 WP_004802302.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63221.54 Da        Isoelectric Point: 5.0086

>NTDB_id=375079 FPL17_RS12435 WP_159885336.1 2712417..2714129(+) (pilB) [Acinetobacter dispersus strain NCCP 16014]
MSGLHTSPKFSGFIRRLVEDGHMPITTMQTAIDAAKKAQQDIVAHLIEQHRVSPLTIAETIAAEFGEPLFDLAVYDTALL
PRDLADNKLIQKHKVVPLIQRGQILYVATSNPTNIEALDAIRFNTKLNIEPIIVEHTKLEKLIEQQFGDSGSFDFGDSED
FDLDIDVNQDTAKDDDEAPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQMATRLAS
RLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKALFMDALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCGQCKEEISIPKQSLLELGF
TEQDLNDPEFKIFQPVGCAECREGYKGRVGIYEVMKVTPEISRLIMEDGNALQIAAASEQAGFNNLRRSGLKKVMQGITS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=375079 FPL17_RS12435 WP_159885336.1 2712417..2714129(+) (pilB) [Acinetobacter dispersus strain NCCP 16014]
ATGTCAGGATTACATACGTCTCCAAAGTTTTCGGGGTTTATTCGACGGTTAGTTGAAGATGGGCATATGCCTATAACAAC
AATGCAAACAGCCATCGATGCTGCTAAAAAAGCACAACAAGATATCGTTGCACATCTGATTGAACAGCATCGCGTATCTC
CATTAACCATTGCTGAAACCATTGCAGCCGAGTTTGGTGAGCCACTTTTCGATTTGGCTGTTTATGATACTGCCTTGTTA
CCTCGTGATCTGGCGGATAATAAGCTAATCCAAAAGCATAAAGTGGTCCCGCTGATTCAACGTGGTCAAATCCTCTATGT
TGCGACCAGCAATCCAACCAATATCGAGGCACTGGATGCGATTCGCTTCAATACCAAACTCAATATTGAACCGATTATTG
TTGAACACACCAAACTCGAAAAACTGATTGAACAGCAGTTTGGCGATTCTGGCAGCTTTGATTTTGGTGATAGTGAAGAC
TTTGATCTCGATATCGATGTGAATCAAGACACTGCCAAAGACGATGATGAGGCGCCTCAAGGGGATGAATCTCCGATTGT
AAAATACATCAATAAGCTATTGATTGATGCGATTCGCATGGGTGCATCCGATTTACATTTCGAACCTTATGAAAAAATGT
ATCGTGTCCGTTATCGCGTTGATGGTGTACTTCGTCAAATCGCCAACCCACCATTACAAATGGCAACCCGTCTCGCCTCA
CGTTTAAAAGTCATGTCACAAATGGACATTTCTGAAAAACGAATGCCGCAAGATGGACGTATTAAACTTAAACTGTCCAA
GACCAAAGCGATTGATTTCCGTGTCAACTCACTCCCCACACTGTTTGGCGAAAAGTTGGTACTGCGTATTCTAGATCCAT
CCAGCGCCATGCTGGGTATTGAAGCTCTCGGCTACGAAGACGAACAAAAAGCTTTATTTATGGATGCACTTGATAAACCT
CAAGGCATGCTCCTGATTACAGGTCCAACGGGCTCTGGTAAAACGGTTTCGCTGTATACGGGATTAAATATTTTAAATAC
GGAAGACACCAATATTTCCACGGCCGAAGATCCAGTCGAAATTAACTTGGAAGGGATTAATCAGGTTAACGTAAACCCAA
AAACAGGACTCACCTTCTCTGCTGCCCTGAAAGCCTTTTTACGTCAAGATCCAGACGTGATCATGGTCGGGGAGATCCGT
GATTTGGAAACAGCAGAAATTGCAATTAAAGCTGCGCAAACAGGTCACATGGTGATGTCGACACTGCATACCAACAGTGC
ACCGGAGACATTGACCCGATTACGCAATATGGGCGTTCCCTCATTCAATATTGCAACTTCCGTCAATCTGGTGATTGCGC
AACGCTTGGCACGCCGCCTCTGTGGTCAATGTAAAGAAGAAATTAGCATACCTAAACAAAGCTTGTTAGAACTCGGCTTT
ACTGAACAAGATTTAAATGATCCCGAGTTTAAAATTTTTCAACCTGTCGGTTGTGCTGAGTGCCGTGAAGGCTATAAAGG
GCGTGTGGGGATTTACGAGGTCATGAAGGTCACACCAGAAATTTCCAGACTGATTATGGAAGATGGTAACGCCTTACAAA
TTGCGGCAGCATCAGAACAAGCAGGTTTTAATAATCTACGTCGTTCAGGTTTGAAAAAGGTCATGCAAGGAATCACCTCT
TTGCAAGAAGTCAACCGAGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

83.012

100

0.832
  pilB Acinetobacter baylyi ADP1

76.708

100

0.768
  pilB Legionella pneumophila strain ERS1305867

54.291

94.035

0.511
  pilF Neisseria gonorrhoeae MS11

49.204

99.123

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

48.381

97.544

0.472
  pilB Vibrio cholerae strain A1552

51.064

90.702

0.463
  pilB Vibrio campbellii strain DS40M4

50.772

90.877

0.461
  pilF Thermus thermophilus HB27

37.5

98.246

0.368
  pilB/pilB1 Synechocystis sp. PCC 6803

33.876

100

0.365

Multiple sequence alignment