Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   FOY97_RS26530 Genome accession   NZ_CP041774
Coordinates   5504057..5505274 (+) Length   405 a.a.
NCBI ID   WP_003116250.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 60503     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5484391..5505274 5504057..5505274 within 0


Gene organization within MGE regions


Location: 5484391..5505274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOY97_RS26405 (FOY97_26405) - 5484391..5485605 (+) 1215 WP_033938764.1 site-specific integrase -
  FOY97_RS26410 (FOY97_26410) - 5485602..5486132 (-) 531 WP_033938765.1 hypothetical protein -
  FOY97_RS26415 (FOY97_26415) - 5486254..5486454 (+) 201 WP_033989250.1 helix-turn-helix domain-containing protein -
  FOY97_RS26420 (FOY97_26420) - 5486451..5486867 (+) 417 WP_033938766.1 hypothetical protein -
  FOY97_RS26425 (FOY97_26425) - 5486860..5487093 (+) 234 WP_023110507.1 hypothetical protein -
  FOY97_RS26430 (FOY97_26430) - 5487090..5487359 (+) 270 WP_033938767.1 hypothetical protein -
  FOY97_RS26435 (FOY97_26435) - 5487356..5487658 (+) 303 WP_033938769.1 hypothetical protein -
  FOY97_RS33250 - 5487661..5488098 (+) 438 WP_306283418.1 hypothetical protein -
  FOY97_RS26445 (FOY97_26445) - 5488137..5488604 (+) 468 WP_033938771.1 hypothetical protein -
  FOY97_RS26450 (FOY97_26450) - 5488890..5489216 (+) 327 Protein_5112 DUF3631 domain-containing protein -
  FOY97_RS26455 (FOY97_26455) - 5489272..5490168 (+) 897 WP_023107349.1 hypothetical protein -
  FOY97_RS26460 (FOY97_26460) - 5490299..5490712 (+) 414 WP_031629315.1 hypothetical protein -
  FOY97_RS26465 (FOY97_26465) - 5490863..5491438 (-) 576 WP_124101441.1 hypothetical protein -
  FOY97_RS26470 (FOY97_26470) - 5492215..5492721 (-) 507 WP_003096122.1 GNAT family N-acetyltransferase -
  FOY97_RS26475 (FOY97_26475) - 5493181..5493423 (-) 243 WP_003096120.1 hypothetical protein -
  FOY97_RS26480 (FOY97_26480) - 5493474..5493743 (+) 270 WP_003096118.1 hypothetical protein -
  FOY97_RS26485 (FOY97_26485) - 5493743..5493979 (+) 237 WP_003096116.1 hypothetical protein -
  FOY97_RS26490 (FOY97_26490) - 5494117..5494353 (-) 237 WP_071536284.1 hypothetical protein -
  FOY97_RS26495 (FOY97_26495) - 5494382..5497144 (-) 2763 WP_123186583.1 RHS repeat domain-containing protein -
  FOY97_RS26500 (FOY97_26500) - 5497159..5498307 (-) 1149 WP_023098865.1 DUF6531 domain-containing protein -
  FOY97_RS26505 (FOY97_26505) - 5500335..5500577 (+) 243 WP_031690159.1 hypothetical protein -
  FOY97_RS26515 (FOY97_26515) - 5500820..5501518 (-) 699 WP_223677410.1 hypothetical protein -
  FOY97_RS26520 (FOY97_26520) - 5501582..5502121 (-) 540 WP_033938465.1 pilin -
  FOY97_RS26525 (FOY97_26525) pilB 5502350..5504053 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FOY97_RS26530 (FOY97_26530) pilC 5504057..5505274 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44051.94 Da        Isoelectric Point: 9.8417

>NTDB_id=374188 FOY97_RS26530 WP_003116250.1 5504057..5505274(+) (pilC) [Pseudomonas aeruginosa strain 60503]
MADKALKTSVFIWEGTDKKGSKVKGELAGQNPMLVKAQLRKQGINPLKVRKKGITLFGAGKKIKPMDIALFTRQMSTMMG
AGVPLLQSFDIIGEGFENPNMRKLVDEIKQDVAAGNSLASSLRKKPIYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVIVVALVVSAILLIKVVPQFQSVFANFGAELPAFTQMVINLSEILQEWWLIVLIGLFAAAFAFR
EVHRRSQKLRDSVDRGLLKLPIIGGILYKSAIARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKNAVTKIKQDVSSGM
QLNFSMRTTNVFPSMAIQMAAIGEESGSLDEMLGKVAGFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=374188 FOY97_RS26530 WP_003116250.1 5504057..5505274(+) (pilC) [Pseudomonas aeruginosa strain 60503]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCATCTGGGAAGGCACTGATAAGAAAGGCAGCAAGGTCAAAGGAGAGTT
GGCCGGGCAAAACCCTATGCTGGTGAAGGCTCAACTGCGCAAACAGGGTATCAACCCACTAAAGGTCCGCAAGAAAGGTA
TCACCCTGTTTGGGGCAGGGAAGAAGATTAAGCCCATGGACATCGCCCTGTTCACTCGGCAGATGTCTACCATGATGGGT
GCCGGCGTACCGTTACTGCAATCTTTTGACATCATCGGCGAAGGATTCGAAAATCCAAACATGCGCAAGCTAGTCGATGA
GATCAAGCAGGATGTTGCCGCCGGTAACAGCTTAGCCAGTTCACTTCGAAAGAAACCCATTTACTTCGATGATCTCTACT
GCAACCTGGTCGATGCTGGCGAACAGTCCGGTGCTTTGGAGACATTATTGGATCGGGTAGCAACTTATAAAGAAAAGACA
GAATCCCTGAAAGCCAAAATTAAAAAGGCCATGACTTATCCCATTGCAGTAATTGTAGTGGCCCTTGTGGTGTCGGCGAT
CCTTCTGATAAAAGTGGTCCCACAGTTCCAGTCCGTATTTGCAAATTTTGGCGCCGAGTTGCCGGCCTTTACTCAAATGG
TCATCAATCTTTCCGAGATTCTTCAAGAGTGGTGGCTCATAGTGCTTATTGGTCTTTTTGCCGCAGCTTTTGCATTTAGG
GAAGTTCATCGGCGCTCGCAAAAACTGAGAGATTCAGTAGATCGGGGCCTGCTGAAACTACCTATCATCGGCGGGATACT
TTACAAATCAGCTATCGCCCGCTACGCCCGAACGCTATCCACTACCTTTGCGGCTGGAGTGCCTCTGGTAGAAGCTCTGG
ACTCCGTTTCCGGAGCAACTGGCAACGTGGTATTCAAGAATGCCGTTACCAAGATCAAACAAGACGTGTCCAGCGGCATG
CAATTGAACTTTTCCATGCGCACCACCAATGTTTTCCCCAGTATGGCTATCCAGATGGCTGCAATTGGCGAGGAATCAGG
TTCGCTAGATGAGATGTTAGGAAAAGTTGCCGGTTTCTATGAGGAAGAAGTCGATAACGCCGTCGACAACCTGACAACGT
TGATGGAGCCGATGATCATGGCCGTGCTCGGCGTACTGGTCGGCGGCCTGATCATCGCTATGTATCTACCGATCTTCCAA
CTGGGTTCCGTTGTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.506

100

0.795
  pilC Acinetobacter baumannii D1279779

60.644

99.753

0.605
  pilC Acinetobacter baylyi ADP1

59.606

100

0.598
  pilC Legionella pneumophila strain ERS1305867

55.051

97.778

0.538
  pilG Neisseria meningitidis 44/76-A

46.269

99.259

0.459
  pilG Neisseria gonorrhoeae MS11

46.02

99.259

0.457
  pilC Vibrio cholerae strain A1552

42.222

100

0.422
  pilC Vibrio campbellii strain DS40M4

40.909

97.778

0.4
  pilC Thermus thermophilus HB27

38.404

99.012

0.38

Multiple sequence alignment