Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FNZ07_RS30795 Genome accession   NZ_CP041745
Coordinates   3911382..3912617 (+) Length   411 a.a.
NCBI ID   WP_372342670.1    Uniprot ID   -
Organism   Paraburkholderia megapolitana strain LMG 23650     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3906382..3917617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FNZ07_RS30765 (FNZ07_30760) cgtA 3906507..3907622 (-) 1116 WP_091010982.1 Obg family GTPase CgtA -
  FNZ07_RS30770 (FNZ07_30765) rpmA 3907800..3908063 (-) 264 WP_091010981.1 50S ribosomal protein L27 -
  FNZ07_RS30775 (FNZ07_30770) rplU 3908088..3908399 (-) 312 WP_091010980.1 50S ribosomal protein L21 -
  FNZ07_RS30780 (FNZ07_30775) - 3908637..3909629 (+) 993 WP_091010979.1 polyprenyl synthetase family protein -
  FNZ07_RS30790 (FNZ07_30785) - 3909852..3911156 (+) 1305 WP_091010978.1 HlyC/CorC family transporter -
  FNZ07_RS30795 (FNZ07_30790) pilF 3911382..3912617 (+) 1236 WP_372342670.1 GspE/PulE family protein Machinery gene
  FNZ07_RS30800 (FNZ07_30795) - 3912614..3913825 (+) 1212 WP_091010976.1 type II secretion system F family protein -
  FNZ07_RS30805 (FNZ07_30800) - 3913854..3914786 (+) 933 WP_091010975.1 A24 family peptidase -
  FNZ07_RS30810 (FNZ07_30805) coaE 3914791..3915396 (+) 606 WP_091010974.1 dephospho-CoA kinase -
  FNZ07_RS30815 (FNZ07_30810) zapD 3915595..3916350 (+) 756 WP_091010973.1 cell division protein ZapD -
  FNZ07_RS30820 (FNZ07_30815) yacG 3916446..3916643 (+) 198 WP_091010972.1 DNA gyrase inhibitor YacG -
  FNZ07_RS30825 (FNZ07_30820) - 3916695..3917111 (-) 417 WP_091010971.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 411 a.a.        Molecular weight: 44142.40 Da        Isoelectric Point: 7.0867

>NTDB_id=373716 FNZ07_RS30795 WP_372342670.1 3911382..3912617(+) (pilF) [Paraburkholderia megapolitana strain LMG 23650]
MATAGTTTSQRTALSDDADDTDSAPAVRLLTDTLREAATRNASDIHVEPAEHGWRIRLRIDGVLHETGKPPAHLRDAFIS
RVKVLARMDIAERRIPQDGRLRLAATKGAFEDYRVNSLPTLFGEKLVLRRLEALPTDLTLDSLGLDAQQRDIVDAAIRAP
HGLVLVTGPTGSGKTLSLYCFLQMLNVASRNLCSVEDPAEIQLAGINQVGVREKAGLTFAIALRAFLRQDPDVIMVGEIR
DEETADVAVKAAQTGHLVLSTLHTNDAPAAIARLIDIGVEPYNLAAALRLVTAQRLVRRLCVSCRVPAKESGAALLAAGF
AREHLGDWHPYTAAGCAACHGIGYRGRIGIHQVMPVPESMRDLIVTRAGTQAISAHAQNDGRVTTLRDAALARVRDGTTS
LAEALAATETQ

Nucleotide


Download         Length: 1236 bp        

>NTDB_id=373716 FNZ07_RS30795 WP_372342670.1 3911382..3912617(+) (pilF) [Paraburkholderia megapolitana strain LMG 23650]
GTGGCAACGGCAGGGACCACAACAAGCCAGCGAACTGCTCTTTCAGACGATGCGGATGACACCGACTCCGCCCCCGCAGT
CCGCCTGCTAACGGACACATTGCGCGAAGCGGCCACGCGTAATGCATCGGACATTCACGTTGAACCGGCGGAACACGGCT
GGCGGATCCGCCTGAGAATCGACGGTGTGCTGCATGAGACCGGCAAGCCGCCCGCTCACTTGCGCGACGCGTTCATCTCA
CGCGTGAAGGTGCTCGCCAGGATGGATATCGCCGAGCGGCGCATTCCGCAGGACGGCCGCCTGCGGCTCGCCGCGACCAA
AGGGGCGTTCGAGGACTATCGCGTCAACTCGCTACCGACACTCTTCGGCGAAAAGCTGGTGCTGCGCCGACTCGAAGCGC
TACCCACCGATCTGACGCTCGACTCGCTCGGGCTCGATGCGCAGCAACGCGATATCGTCGACGCCGCTATCCGCGCACCG
CACGGCCTCGTGCTCGTCACCGGTCCCACGGGCAGCGGTAAAACGCTGTCGTTGTACTGCTTCCTGCAGATGCTGAATGT
CGCGTCGCGCAATCTCTGTTCGGTGGAAGATCCGGCGGAGATCCAGCTTGCCGGCATCAACCAGGTCGGCGTGCGCGAGA
AAGCGGGGCTGACCTTCGCGATCGCACTGCGCGCGTTTTTGCGGCAGGACCCGGACGTGATCATGGTCGGCGAAATCCGC
GACGAAGAAACCGCCGATGTCGCGGTGAAAGCCGCACAGACCGGCCACCTCGTACTTTCCACCTTGCATACGAACGACGC
TCCTGCGGCGATCGCGCGGCTCATCGATATCGGCGTCGAGCCGTACAACCTCGCGGCGGCGTTGCGGCTCGTAACAGCGC
AGCGGCTCGTGCGCCGTCTCTGCGTGTCGTGCCGCGTACCGGCGAAGGAATCGGGCGCGGCACTGCTCGCGGCCGGCTTT
GCGCGCGAGCATCTCGGCGACTGGCATCCATATACAGCGGCTGGATGCGCGGCGTGTCATGGAATCGGTTATCGCGGGCG
CATCGGCATTCACCAGGTGATGCCGGTTCCGGAGTCGATGCGCGACCTGATCGTCACGCGTGCGGGCACCCAGGCGATCT
CGGCGCATGCGCAGAACGACGGACGCGTCACGACGCTGCGCGATGCGGCACTCGCCCGCGTGCGCGATGGCACGACGAGC
CTCGCCGAAGCCCTCGCCGCCACGGAGACCCAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(33-299)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

50.886

96.107

0.489
  pilB Vibrio cholerae strain A1552

50.262

92.944

0.467
  pilB Acinetobacter baumannii D1279779

48.462

94.891

0.46
  pilB Acinetobacter baylyi ADP1

48.168

92.944

0.448
  pilB Vibrio parahaemolyticus RIMD 2210633

46.329

96.107

0.445
  pilB/pilB1 Synechocystis sp. PCC 6803

44.39

99.757

0.443
  pilB Vibrio campbellii strain DS40M4

47.258

93.187

0.44
  pilB Legionella pneumophila strain ERS1305867

46.094

93.431

0.431
  pilF Thermus thermophilus HB27

43.59

94.891

0.414
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.646

97.567

0.406
  pilB Haemophilus influenzae 86-028NP

41.495

94.404

0.392
  pilB Haemophilus influenzae Rd KW20

45.238

81.752

0.37
  pilB Glaesserella parasuis strain SC1401

45.455

80.292

0.365

Multiple sequence alignment