Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G6000_RS14605 Genome accession   NZ_CP049198
Coordinates   3071803..3073188 (+) Length   461 a.a.
NCBI ID   WP_001025167.1    Uniprot ID   -
Organism   Escherichia coli strain E686     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3066803..3078188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G6000_RS14580 aroP 3067351..3068724 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  G6000_RS14585 ampE 3068767..3069621 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  G6000_RS14590 ampD 3069618..3070169 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G6000_RS14595 nadC 3070257..3071150 (+) 894 WP_001135153.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G6000_RS14600 pilA 3071353..3071793 (+) 441 WP_020233150.1 prepilin peptidase-dependent pilin Machinery gene
  G6000_RS14605 pilB 3071803..3073188 (+) 1386 WP_001025167.1 type II secretion system protein GspE Machinery gene
  G6000_RS14610 hofC 3073178..3074380 (+) 1203 WP_021522995.1 protein transport protein HofC -
  G6000_RS14615 guaC 3074415..3075458 (-) 1044 WP_001217337.1 GMP reductase -
  G6000_RS24595 - 3075614..3075658 (-) 45 WP_120795372.1 protein YacM -
  G6000_RS14620 coaE 3075683..3076303 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  G6000_RS14625 zapD 3076303..3077046 (+) 744 WP_001194736.1 cell division protein ZapD -
  G6000_RS14630 yacG 3077056..3077253 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  G6000_RS14635 mutT 3077455..3077853 (-) 399 WP_000736024.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50655.35 Da        Isoelectric Point: 6.6256

>NTDB_id=373101 G6000_RS14605 WP_001025167.1 3071803..3073188(+) (pilB) [Escherichia coli strain E686]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGFYGRTALFE
VLPITPIIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=373101 G6000_RS14605 WP_001025167.1 3071803..3073188(+) (pilB) [Escherichia coli strain E686]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTATTCATGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATACGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGGAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCCCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATTC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCGCATTGTCGCCGGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCGCTGGCAAGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTACCCATAACACCGATCATACGCCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACACACGCCCGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.469

100

0.416
  pilB Legionella pneumophila strain ERS1305867

49.74

83.297

0.414
  pilB Haemophilus influenzae 86-028NP

39.915

100

0.408
  pilB Vibrio campbellii strain DS40M4

48.187

83.731

0.403
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

84.599

0.393
  pilB Vibrio cholerae strain A1552

46.41

84.599

0.393
  pilB Acinetobacter baylyi ADP1

45.22

83.948

0.38
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilB Haemophilus influenzae Rd KW20

43.928

83.948

0.369
  pilB Acinetobacter baumannii D1279779

43.864

83.08

0.364
  pilF Thermus thermophilus HB27

41.032

88.286

0.362