Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G6000_RS14600 Genome accession   NZ_CP049198
Coordinates   3071353..3071793 (+) Length   146 a.a.
NCBI ID   WP_020233150.1    Uniprot ID   -
Organism   Escherichia coli strain E686     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3066353..3076793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G6000_RS14580 aroP 3067351..3068724 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  G6000_RS14585 ampE 3068767..3069621 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  G6000_RS14590 ampD 3069618..3070169 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G6000_RS14595 nadC 3070257..3071150 (+) 894 WP_001135153.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G6000_RS14600 pilA 3071353..3071793 (+) 441 WP_020233150.1 prepilin peptidase-dependent pilin Machinery gene
  G6000_RS14605 pilB 3071803..3073188 (+) 1386 WP_001025167.1 type II secretion system protein GspE Machinery gene
  G6000_RS14610 hofC 3073178..3074380 (+) 1203 WP_021522995.1 protein transport protein HofC -
  G6000_RS14615 guaC 3074415..3075458 (-) 1044 WP_001217337.1 GMP reductase -
  G6000_RS24595 - 3075614..3075658 (-) 45 WP_120795372.1 protein YacM -
  G6000_RS14620 coaE 3075683..3076303 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=373100 G6000_RS14600 WP_020233150.1 3071353..3071793(+) (pilA) [Escherichia coli strain E686]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTNMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=373100 G6000_RS14600 WP_020233150.1 3071353..3071793(+) (pilA) [Escherichia coli strain E686]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCAACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGTAATGGCATCCCCTCGCCTACCACTACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGTTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTTCGCTTTGATGACGCAAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363