Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FNT16_RS03885 Genome accession   NZ_CP041660
Coordinates   994210..995901 (+) Length   563 a.a.
NCBI ID   WP_143870311.1    Uniprot ID   -
Organism   Catenovulum sediminis strain WS1-A     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 989210..1000901
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FNT16_RS03855 moaD 990077..990325 (-) 249 WP_143870306.1 molybdopterin converting factor subunit 1 -
  FNT16_RS03860 moaC 990336..990809 (-) 474 WP_143870307.1 cyclic pyranopterin monophosphate synthase MoaC -
  FNT16_RS03865 moaB 990812..991357 (-) 546 WP_143870308.1 molybdenum cofactor biosynthesis protein B -
  FNT16_RS03870 - 991380..991985 (-) 606 WP_143870309.1 molybdenum cofactor guanylyltransferase -
  FNT16_RS03875 moaA 992006..992980 (-) 975 WP_143870310.1 GTP 3',8-cyclase MoaA -
  FNT16_RS20960 - 993467..993985 (+) 519 WP_342779157.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FNT16_RS03885 pilB 994210..995901 (+) 1692 WP_143870311.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FNT16_RS03890 pilC 995922..997166 (+) 1245 WP_143870312.1 type II secretion system F family protein Machinery gene
  FNT16_RS03895 pilD 997166..998104 (+) 939 WP_143870313.1 A24 family peptidase Machinery gene
  FNT16_RS03900 coaE 998104..998709 (+) 606 WP_143870314.1 dephospho-CoA kinase -
  FNT16_RS03905 zapD 998766..999512 (+) 747 WP_143870315.1 cell division protein ZapD -
  FNT16_RS03910 yacG 999506..999727 (+) 222 WP_143870316.1 DNA gyrase inhibitor YacG -
  FNT16_RS03915 ubiA 999906..1000772 (-) 867 WP_143870317.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62764.91 Da        Isoelectric Point: 5.2505

>NTDB_id=372825 FNT16_RS03885 WP_143870311.1 994210..995901(+) (pilB) [Catenovulum sediminis strain WS1-A]
MAAIDFRIFSGGLLDLHQQEEIRSQDLKSHIEAIQYIRKHGWYSELELAEEISQRFHTPLFDLNYFDIKSIKPELLKEKL
SEKHKVLPLFLRGRTLFLATVNPSSASAQEDFQFSTGYNVEYLCVEASKLNQAIETAYETEEGQLDLDDFDEQELAGIEV
GQDKTVEENESIENDDAPIVVYINKILLDAIKRGASDLHFEPYEKSYRIRFRIDGILHEVASPPVSLSTRLAARLKVMSR
LDIAEKRKPQDGRIKLKISAKKSIDFRVSTLPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYLQALEKPQGMILVT
GPTGSGKTVSLYTGLNILNTSETNISTAEDPVEINLEGINQVQVNPKAGLTFAEALRSFLRQDPDIVMVGEIRDLETAEI
AIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPSFNIASSVSLIIAQRLARRLCKHCKKPVEVLPTEQELLDFGFSAAQL
KDLTLYEPVGCELCSSGYKGRVGIYEVMPISANMANIIMREGNSIEIAKQAQQEKIANLRQSGIKKAISGMTSLGEVSRV
TSY

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=372825 FNT16_RS03885 WP_143870311.1 994210..995901(+) (pilB) [Catenovulum sediminis strain WS1-A]
ATGGCAGCAATAGATTTTCGTATATTTTCCGGCGGTTTATTAGACCTTCACCAACAAGAAGAAATTCGCTCGCAAGACTT
AAAGTCACATATTGAGGCGATTCAATACATCCGTAAGCATGGCTGGTATAGTGAATTAGAACTCGCAGAAGAAATCAGCC
AACGATTTCACACCCCTCTTTTTGATTTAAATTATTTTGATATTAAATCTATCAAGCCTGAACTGTTAAAAGAAAAATTA
TCAGAAAAACATAAAGTTTTACCTTTATTCTTACGTGGGCGAACGCTTTTTCTAGCAACCGTCAATCCATCCAGTGCCAG
CGCACAAGAAGATTTTCAATTTAGCACAGGCTACAACGTTGAGTATTTATGCGTAGAAGCCAGTAAGTTAAATCAAGCAA
TTGAAACCGCCTATGAAACAGAAGAAGGCCAGCTGGATTTAGATGATTTTGATGAACAGGAATTAGCTGGCATTGAAGTT
GGCCAAGATAAAACCGTCGAAGAAAATGAATCAATCGAAAATGACGATGCGCCGATTGTTGTCTATATTAACAAAATTTT
ACTCGACGCCATTAAGCGTGGCGCTTCGGATTTACACTTCGAACCGTATGAAAAAAGCTATCGGATCCGATTTCGTATTG
ATGGCATTTTACATGAGGTTGCCTCGCCGCCCGTTTCTTTGTCTACCCGCCTTGCCGCTCGATTAAAAGTGATGTCTCGG
TTAGACATAGCCGAAAAGAGAAAACCGCAAGATGGTCGCATAAAACTAAAGATATCAGCTAAAAAGTCCATCGATTTCCG
TGTTTCTACCCTACCAACTTTGTGGGGCGAAAAAATCGTAATGCGTATCTTGGATTCCAGTAGCGCCATGCTGGGTATTG
ATGTGTTAGGTTATGAGCCAGAGCAAAAAAAATTATATTTGCAAGCCTTAGAAAAACCACAAGGTATGATTTTGGTGACG
GGCCCAACAGGGAGCGGTAAAACCGTATCTTTATATACGGGGTTAAATATTTTAAACACCTCAGAAACAAATATCTCGAC
GGCAGAAGATCCGGTTGAAATTAACCTTGAAGGTATTAACCAGGTGCAAGTCAACCCTAAAGCTGGGTTAACCTTTGCAG
AAGCATTGCGCTCATTTTTACGTCAGGATCCGGATATTGTTATGGTGGGTGAGATCCGAGATTTAGAAACGGCAGAAATA
GCAATAAAAGCGGCACAAACAGGTCACCTTGTGCTTTCGACGCTGCATACCAACTCAGCCGCTGAAACGCTCACGCGTCT
TTTAAATATGGGTGTACCGAGTTTTAACATTGCCAGTTCAGTCAGCCTTATCATCGCACAACGATTAGCCAGACGCTTAT
GTAAACATTGTAAAAAACCCGTTGAAGTATTGCCCACAGAGCAAGAATTATTGGATTTTGGCTTTTCAGCTGCACAATTA
AAAGATTTAACACTTTATGAACCCGTAGGTTGCGAACTCTGCTCATCTGGATACAAGGGGCGAGTCGGTATTTATGAAGT
CATGCCAATCAGCGCGAACATGGCAAATATTATTATGCGAGAAGGCAACTCAATAGAAATTGCCAAACAAGCACAACAAG
AGAAAATTGCTAATTTACGCCAGTCAGGTATTAAAAAGGCGATCTCAGGCATGACCAGTTTAGGCGAAGTTAGCCGGGTC
ACTAGCTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(185-452)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.127

100

0.584
  pilB Acinetobacter baumannii D1279779

57.672

100

0.581
  pilB Legionella pneumophila strain ERS1305867

57.014

98.757

0.563
  pilB Vibrio parahaemolyticus RIMD 2210633

53.333

98.579

0.526
  pilB Vibrio campbellii strain DS40M4

53.058

98.757

0.524
  pilB Vibrio cholerae strain A1552

55.662

92.54

0.515
  pilF Neisseria gonorrhoeae MS11

49.912

100

0.501
  pilB/pilB1 Synechocystis sp. PCC 6803

37.828

100

0.384
  pilF Thermus thermophilus HB27

39.922

91.652

0.366

Multiple sequence alignment