Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FKL89_RS04540 Genome accession   NZ_CP041352
Coordinates   991319..993034 (-) Length   571 a.a.
NCBI ID   WP_156861647.1    Uniprot ID   -
Organism   Casimicrobium huifangae strain SJ-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 986319..998034
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FKL89_RS04510 - 986932..987339 (+) 408 WP_272953732.1 NUDIX domain-containing protein -
  FKL89_RS04515 - 987503..987688 (-) 186 WP_156861642.1 DNA gyrase inhibitor YacG -
  FKL89_RS04520 zapD 987713..988507 (-) 795 WP_156861643.1 cell division protein ZapD -
  FKL89_RS04525 coaE 988504..989163 (-) 660 WP_156861644.1 dephospho-CoA kinase -
  FKL89_RS04530 pilD 989178..990020 (-) 843 WP_156861645.1 prepilin peptidase Machinery gene
  FKL89_RS04535 pilC 990029..991276 (-) 1248 WP_156861646.1 type II secretion system F family protein Machinery gene
  FKL89_RS04540 pilB 991319..993034 (-) 1716 WP_156861647.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FKL89_RS04545 ffh 993199..994584 (+) 1386 WP_156861648.1 signal recognition particle protein -
  FKL89_RS04550 ybiB 994782..995708 (+) 927 WP_156861649.1 DNA-binding protein YbiB -
  FKL89_RS04555 cobA 995683..996483 (+) 801 WP_156861650.1 uroporphyrinogen-III C-methyltransferase -
  FKL89_RS04560 - 996602..996970 (+) 369 WP_238363485.1 sirohydrochlorin chelatase -
  FKL89_RS04565 - 997028..997666 (-) 639 WP_156861652.1 bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62027.26 Da        Isoelectric Point: 5.1248

>NTDB_id=371399 FKL89_RS04540 WP_156861647.1 991319..993034(-) (pilB) [Casimicrobium huifangae strain SJ-1]
MATSSASVLLSGLGRALVQHDILRQVDAEQVQTQANATSVTFVEELVTSKRITAGKLAEFAASTFGVPLFDLSAFDTTTI
NQEWIDVKLITARRALPLHKRGNRLFVALSDPTNLQALEEFRFKTSLIAEPIVVEDDKLGRAIAAVAERNDKTLASLDVG
DDIDLDIKVEDGPAAEEDTGDVDDAPVVRYIQKILLDAINSSVSDIHFEPYEKFYRIRYRLDGVLVEVAKPPLVIKDKIA
SRIKVISKLDISEKRVPQDGRMKLQLTKNRSIDFRVSTLPTLYGEKIVMRILDSSNAMLGIDALGYDADQKAALLEAISR
PYGMILVTGPTGSGKTISLYTCLGLLNKPGINISTAEDPAEINLPGVNQVNVNDKAGLTFAAALKSFLRQDPDIIMVGEI
RDLETAEIAIKAAQTGHLVLSTLHTNDAPATLVRLSNMGVAAFNIASSVLLITAQRLARRLCASCKQPSDIPVDVLLTAG
FKESDIDGTWAPYRPVGCDVCKGTGYKGRVGIYEVMPITEDMQRIIMSNGNTLDIAAQAKREGVRNLRESGLLKVKQGVT
SLEEIEAVTNA

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=371399 FKL89_RS04540 WP_156861647.1 991319..993034(-) (pilB) [Casimicrobium huifangae strain SJ-1]
GTGGCCACCTCGTCCGCCTCTGTATTGCTGTCCGGTCTTGGCCGTGCGCTCGTCCAGCATGACATTCTTCGTCAGGTGGA
TGCCGAGCAGGTACAGACGCAAGCCAATGCAACCTCGGTGACTTTTGTCGAAGAGCTGGTCACCTCCAAACGCATCACGG
CCGGCAAACTGGCCGAGTTCGCCGCCAGCACTTTTGGCGTGCCGCTGTTCGATCTGTCGGCTTTCGACACGACAACGATC
AATCAGGAATGGATCGACGTCAAACTGATCACTGCGCGCCGTGCGCTGCCACTGCACAAGCGCGGTAACCGCCTGTTCGT
CGCGCTGTCGGACCCGACCAATCTGCAGGCGCTGGAAGAATTTCGCTTCAAGACCAGTCTGATCGCCGAGCCCATCGTTG
TCGAAGACGACAAACTCGGTCGTGCGATCGCTGCTGTTGCTGAGCGCAATGACAAGACGCTCGCTTCGCTCGATGTCGGC
GATGATATCGACCTCGATATCAAGGTTGAGGACGGGCCGGCAGCGGAAGAAGATACGGGTGACGTTGACGATGCACCGGT
CGTCCGCTATATCCAGAAAATCCTGCTTGATGCGATCAATTCAAGCGTTTCCGACATCCACTTCGAGCCGTACGAAAAGT
TCTATCGCATCCGCTACCGCCTCGATGGCGTGCTCGTCGAAGTCGCGAAGCCGCCACTGGTCATCAAGGACAAGATCGCC
TCGCGCATCAAGGTGATCTCGAAACTGGACATTTCGGAAAAGCGCGTGCCGCAAGACGGCCGCATGAAGCTGCAGCTTAC
CAAGAACCGCTCGATCGACTTCCGCGTCAGCACACTGCCGACGCTCTACGGCGAAAAGATCGTGATGCGTATTCTCGACT
CGTCGAATGCGATGCTCGGTATCGATGCGCTGGGCTACGACGCGGATCAGAAAGCTGCACTGCTGGAGGCCATTTCACGT
CCCTACGGCATGATCCTGGTCACCGGGCCAACAGGCTCCGGCAAGACCATATCGCTCTACACCTGCCTGGGCCTGCTGAA
CAAGCCGGGCATCAACATCTCGACAGCAGAAGATCCGGCTGAAATCAATCTGCCAGGCGTCAATCAGGTTAACGTCAATG
ACAAAGCTGGACTGACCTTCGCTGCGGCGCTGAAATCCTTCCTGCGCCAGGACCCCGACATCATCATGGTCGGCGAAATT
CGCGACCTGGAAACGGCTGAAATCGCCATCAAGGCAGCACAAACCGGTCACCTGGTGCTGTCGACGTTACACACCAACGA
CGCGCCAGCGACACTGGTGCGGCTCTCCAACATGGGCGTGGCCGCCTTCAATATCGCCTCGAGCGTGCTGCTAATCACCG
CCCAGCGCCTTGCGCGCCGTCTGTGCGCCAGCTGCAAGCAACCATCCGACATTCCGGTGGATGTGCTACTCACCGCCGGG
TTCAAGGAGTCAGACATCGATGGCACCTGGGCGCCCTACCGTCCGGTGGGATGCGATGTCTGCAAAGGCACTGGCTACAA
GGGCCGCGTCGGTATCTATGAGGTGATGCCGATCACCGAAGACATGCAACGGATCATCATGAGCAATGGCAACACCCTCG
ACATTGCGGCGCAGGCGAAACGCGAAGGGGTACGCAACCTGCGAGAATCCGGTCTGCTCAAAGTCAAACAGGGTGTCACG
TCGCTGGAGGAGATCGAGGCAGTGACAAATGCCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-146)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.959

100

0.567
  pilB Acinetobacter baylyi ADP1

56.206

98.774

0.555
  pilB Legionella pneumophila strain ERS1305867

51.993

100

0.525
  pilF Neisseria gonorrhoeae MS11

53.119

98.249

0.522
  pilB Vibrio parahaemolyticus RIMD 2210633

50

100

0.501
  pilB Vibrio cholerae strain A1552

50.176

99.475

0.499
  pilB Vibrio campbellii strain DS40M4

48.168

100

0.483
  pilB/pilB1 Synechocystis sp. PCC 6803

37.748

100

0.399
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.832

92.644

0.378
  pilF Thermus thermophilus HB27

37.011

98.424

0.364

Multiple sequence alignment