Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   FKL89_RS04530 Genome accession   NZ_CP041352
Coordinates   989178..990020 (-) Length   280 a.a.
NCBI ID   WP_156861645.1    Uniprot ID   -
Organism   Casimicrobium huifangae strain SJ-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 984178..995020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FKL89_RS04500 argJ 984741..985979 (+) 1239 WP_156861639.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  FKL89_RS04505 - 986018..986896 (+) 879 WP_156861640.1 ATP-binding protein -
  FKL89_RS04510 - 986932..987339 (+) 408 WP_272953732.1 NUDIX domain-containing protein -
  FKL89_RS04515 - 987503..987688 (-) 186 WP_156861642.1 DNA gyrase inhibitor YacG -
  FKL89_RS04520 zapD 987713..988507 (-) 795 WP_156861643.1 cell division protein ZapD -
  FKL89_RS04525 coaE 988504..989163 (-) 660 WP_156861644.1 dephospho-CoA kinase -
  FKL89_RS04530 pilD 989178..990020 (-) 843 WP_156861645.1 prepilin peptidase Machinery gene
  FKL89_RS04535 pilC 990029..991276 (-) 1248 WP_156861646.1 type II secretion system F family protein Machinery gene
  FKL89_RS04540 pilB 991319..993034 (-) 1716 WP_156861647.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FKL89_RS04545 ffh 993199..994584 (+) 1386 WP_156861648.1 signal recognition particle protein -

Sequence

Protein


Download         Length: 280 a.a.        Molecular weight: 30413.15 Da        Isoelectric Point: 7.6033

>NTDB_id=371397 FKL89_RS04530 WP_156861645.1 989178..990020(-) (pilD) [Casimicrobium huifangae strain SJ-1]
MQALFAQEPWLFLICAGVFGALIGSFLNVVIYRLPVMMEHAWQDELADAQGNEPAPRERFNLVVPRSRCGQCGHAVTALE
NVPIISWLALRGRCSACGTRISARYPLVEAATAALFVACAWHFGFTFQALAAMTFCAALVTLTGIDLDTQLLPDQLTLPL
LWLGLLVNLFAMFAHLPDAVIGAAAGYLVLWSVYWLFKLVTGREGMGYGDFKLLGALGAWFGWQALPMLLLVSSVVGAVI
GIAILLVQKKGRHTAIAFGPYLAIAGIITLFFGPLLRQLF

Nucleotide


Download         Length: 843 bp        

>NTDB_id=371397 FKL89_RS04530 WP_156861645.1 989178..990020(-) (pilD) [Casimicrobium huifangae strain SJ-1]
TTGCAGGCACTTTTCGCACAGGAGCCTTGGCTGTTCCTGATTTGTGCCGGCGTGTTCGGCGCGCTCATCGGCAGTTTTTT
GAACGTCGTCATTTACCGCTTGCCGGTGATGATGGAACACGCCTGGCAAGATGAACTTGCCGATGCCCAGGGAAATGAGC
CCGCCCCGCGCGAGCGATTCAATCTGGTCGTCCCGCGTTCACGTTGTGGCCAGTGCGGCCACGCCGTCACCGCCCTGGAA
AATGTCCCAATCATTTCCTGGCTTGCCTTGCGCGGCCGCTGCAGCGCTTGCGGGACCCGCATCAGCGCCCGCTACCCGCT
GGTTGAAGCGGCGACAGCCGCCTTGTTCGTTGCATGCGCCTGGCACTTCGGGTTTACCTTTCAGGCGCTCGCCGCGATGA
CGTTCTGTGCAGCGCTGGTCACCTTGACTGGCATCGATCTCGATACCCAGCTACTGCCAGACCAGCTGACATTGCCGCTC
TTGTGGCTTGGCCTGCTGGTCAACCTGTTCGCGATGTTCGCGCACTTGCCGGATGCCGTGATCGGTGCTGCGGCAGGTTA
TCTGGTGCTATGGTCGGTCTACTGGCTGTTCAAGCTGGTTACCGGACGTGAAGGCATGGGTTATGGTGACTTCAAGCTGC
TCGGAGCGCTCGGCGCCTGGTTTGGCTGGCAAGCGCTTCCCATGCTGCTGCTAGTGTCATCGGTGGTTGGCGCGGTAATT
GGTATCGCCATCCTGCTGGTGCAGAAGAAGGGACGGCACACGGCGATCGCCTTTGGTCCGTACCTCGCGATCGCCGGCAT
CATCACGCTGTTCTTTGGTCCGCTGCTGCGGCAACTCTTCTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(133-242)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.109

97.857

0.539
  pilD Vibrio campbellii strain DS40M4

54.579

97.5

0.532
  pilD Neisseria gonorrhoeae MS11

50.391

91.429

0.461
  pilD Acinetobacter baumannii D1279779

44.643

100

0.446
  pilD Acinetobacter nosocomialis M2

44.286

100

0.443

Multiple sequence alignment