Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FLM48_RS20145 Genome accession   NZ_CP041329
Coordinates   4576854..4578560 (+) Length   568 a.a.
NCBI ID   WP_179025909.1    Uniprot ID   -
Organism   Shewanella sp. Scap07     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4571854..4583560
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLM48_RS20125 (FLM48_20270) ampE 4572470..4573321 (-) 852 WP_179025908.1 beta-lactamase regulator AmpE -
  FLM48_RS20130 (FLM48_20275) ampD 4573598..4574155 (-) 558 WP_256870987.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FLM48_RS20135 (FLM48_20280) nadC 4574472..4575356 (+) 885 WP_028773322.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FLM48_RS20140 (FLM48_20285) - 4576365..4576766 (+) 402 WP_179026445.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FLM48_RS20145 (FLM48_20290) pilB 4576854..4578560 (+) 1707 WP_179025909.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FLM48_RS20150 (FLM48_20295) pilC 4578622..4579881 (+) 1260 WP_179025910.1 type II secretion system F family protein Machinery gene
  FLM48_RS20155 (FLM48_20300) pilD 4579965..4580888 (+) 924 WP_179025911.1 A24 family peptidase Machinery gene
  FLM48_RS20160 (FLM48_20305) coaE 4580898..4581506 (+) 609 WP_179025912.1 dephospho-CoA kinase -
  FLM48_RS20165 (FLM48_20310) zapD 4581508..4582242 (+) 735 WP_179025913.1 cell division protein ZapD -
  FLM48_RS20170 (FLM48_20315) yacG 4582293..4582511 (+) 219 WP_028773314.1 DNA gyrase inhibitor YacG -
  FLM48_RS20175 (FLM48_20320) mutT 4582508..4582903 (+) 396 WP_179025914.1 8-oxo-dGTP diphosphatase MutT -
  FLM48_RS22825 - 4582990..4583115 (-) 126 WP_256870988.1 hypothetical protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62731.71 Da        Isoelectric Point: 5.1717

>NTDB_id=371233 FLM48_RS20145 WP_179025909.1 4576854..4578560(+) (pilB) [Shewanella sp. Scap07]
MPTSGLHLGLSTLFIRKQLLSEEQIATAIAKSRHSKLSLVSTIVSEKLISARAIAELCYEEYGTPLLDLDEFDVQSIPED
FLNKKLIEKHKCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEDDKLSSALEKVLEEDISALDLDGIDDAS
LADIEVTDTDKREDDQKADSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRALYEEMLAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTEARNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSASETLTRLVNMGVPGYNIASSVNLIIAQRLARRLCPECKQAEDVPEHELKALGFN
DTQIGEGFTIFKPKGCDHCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIANQAKDQGMRDLRDSGLLKVIHGVTSIA
EINRVTSF

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=371233 FLM48_RS20145 WP_179025909.1 4576854..4578560(+) (pilB) [Shewanella sp. Scap07]
ATGCCAACCAGTGGTTTACACCTTGGACTTTCAACACTATTTATTCGCAAGCAACTCCTCAGCGAAGAACAGATCGCAAC
TGCGATAGCCAAGTCTCGCCACTCTAAACTCTCTTTAGTGTCTACCATTGTCAGTGAAAAACTGATATCTGCTCGTGCAA
TTGCTGAACTCTGCTATGAAGAATATGGTACCCCGCTTCTGGATCTCGATGAGTTTGACGTTCAAAGTATTCCGGAAGAT
TTTCTTAACAAAAAGCTGATAGAAAAACATAAATGCTTACCGCTGTTTAAACGCGGCAATCGTTTATATATTGGTACCTC
CGATCCCACCAATATTGCAGCGCTAGAGGACTTTCAATTTAGTGCAGGTCTGCATGCTGAAGCTATTTTAGTTGAAGATG
ACAAGCTAAGCTCCGCGCTGGAAAAAGTGCTTGAAGAAGATATTAGTGCGCTGGATTTAGACGGCATTGATGATGCTTCT
CTTGCCGATATTGAAGTCACCGATACCGATAAGCGAGAGGACGACCAAAAAGCCGATAGTGACGACGCCCCTATCGTTAT
CTATATCAATAAAATTCTTACCGATGCCATTCGTAAAGGTGCGTCAGATTTGCATTTTGAGCCCTACGAAAAGCGTTATC
GTATCCGTTTCCGCATTGATGGTATTTTACATGAGGTTTCAGAACCCCCAGTTAATTTAAGCGGCCGTATTTCTGCGCGC
TTAAAGGTAATGTCAAAACTTGATATTGCTGAGCGCCGGGTACCGCAAGATGGCCGCATTAAAATGAAGCTATCACGTAC
CAAGTCTATCGATTTTCGTGTCAGCACCTTGCCGACAATCTGGGGCGAAAAGATCGTAATGCGTATTTTGGACTCCTCTT
CTGCGCAGCTTGGCATTGAAAAGCTCGGCTATGAGGATGACCAACGTGCTTTGTATGAGGAAATGTTGGCCAAACCCCAA
GGGATGATATTAGTTACAGGCCCCACAGGTTCAGGTAAAACCGTATCACTCTATACAGGGCTAAATATCCTCAATACCGA
AGCTCGTAATATCTCTACTGCAGAAGACCCAGTCGAAATTAACTTAGAGGGTGTGAATCAGGTCCATATTAACTTGAAAG
CAGGTCTTACCTTCGCATCTGCGTTGCGCTCTTTTTTACGTCAAGATCCCGATGTAGTGATGGTCGGGGAAATCCGTGAT
TTAGAAACTGCCGAAATCGCCATTAAGGCCGCACAAACTGGTCACTTAGTACTGTCCACACTGCATACTAATTCAGCTTC
AGAAACGTTAACTCGTTTGGTCAATATGGGCGTGCCGGGATATAACATTGCCAGCTCAGTTAACTTAATTATTGCTCAGC
GTCTGGCTCGTAGACTTTGCCCTGAATGCAAACAAGCCGAAGATGTGCCAGAGCATGAACTTAAAGCGCTAGGATTTAAT
GATACGCAAATCGGTGAAGGCTTTACTATATTTAAACCTAAAGGTTGCGATCATTGTTCTGGTGGCTATAAAGGGCGAGT
GGGTATATACGAAGTAATGAAAATGTCAGATGAGATTGCCCGCACCATTATGGAAGGTGGTAACTCTTTGCAAATCGCTA
ACCAAGCGAAAGATCAAGGTATGCGTGACTTGCGTGATTCAGGATTGTTAAAAGTCATTCACGGTGTCACTAGCATCGCT
GAAATTAATCGTGTCACCAGTTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(16-144)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.467

98.768

0.577
  pilB Acinetobacter baumannii D1279779

56.79

99.824

0.567
  pilB Legionella pneumophila strain ERS1305867

55.477

99.648

0.553
  pilB Vibrio parahaemolyticus RIMD 2210633

51.399

100

0.518
  pilB Vibrio cholerae strain A1552

51.423

98.944

0.509
  pilB Vibrio campbellii strain DS40M4

49.556

99.12

0.491
  pilF Neisseria gonorrhoeae MS11

47.434

99.472

0.472
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.522

98.063

0.368
  pilF Thermus thermophilus HB27

36.814

99.472

0.366
  pilB/pilB1 Synechocystis sp. PCC 6803

39.238

92.43

0.363

Multiple sequence alignment