Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   FLM48_RS20155 Genome accession   NZ_CP041329
Coordinates   4579965..4580888 (+) Length   307 a.a.
NCBI ID   WP_179025911.1    Uniprot ID   -
Organism   Shewanella sp. Scap07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4574965..4585888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLM48_RS20140 (FLM48_20285) - 4576365..4576766 (+) 402 WP_179026445.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FLM48_RS20145 (FLM48_20290) pilB 4576854..4578560 (+) 1707 WP_179025909.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FLM48_RS20150 (FLM48_20295) pilC 4578622..4579881 (+) 1260 WP_179025910.1 type II secretion system F family protein Machinery gene
  FLM48_RS20155 (FLM48_20300) pilD 4579965..4580888 (+) 924 WP_179025911.1 A24 family peptidase Machinery gene
  FLM48_RS20160 (FLM48_20305) coaE 4580898..4581506 (+) 609 WP_179025912.1 dephospho-CoA kinase -
  FLM48_RS20165 (FLM48_20310) zapD 4581508..4582242 (+) 735 WP_179025913.1 cell division protein ZapD -
  FLM48_RS20170 (FLM48_20315) yacG 4582293..4582511 (+) 219 WP_028773314.1 DNA gyrase inhibitor YacG -
  FLM48_RS20175 (FLM48_20320) mutT 4582508..4582903 (+) 396 WP_179025914.1 8-oxo-dGTP diphosphatase MutT -
  FLM48_RS22825 - 4582990..4583115 (-) 126 WP_256870988.1 hypothetical protein -

Sequence

Protein


Download         Length: 307 a.a.        Molecular weight: 33887.49 Da        Isoelectric Point: 7.2373

>NTDB_id=371235 FLM48_RS20155 WP_179025911.1 4579965..4580888(+) (pilD) [Shewanella sp. Scap07]
MNSLLIQFQSTMLELPWLFISLAFVFAATIGSFLNVVIHRLPVMMKREWQQECNQYLNEYHQELIAPIAKKLGKPLDNYP
EKYNLVVPVSACPKCKTNIKPWHNLPILGWLLLQGKCASCDAPISARYPVFELFTGAMVATLAWHFGPSLQFAMAALLTF
ALVALTGIDLDEMLLPDQITMPLLWLGLIININGLFTPLTDALIGAVAGYLSLWSVYWLFKIATGKEGMGYGDFKLMAVF
GAWLGSQMLPLIILLSSLVGAIVGIMLIINKKLNSGNPIPFGPYIAAAGWIALLWGEPIVNAYLASL

Nucleotide


Download         Length: 924 bp        

>NTDB_id=371235 FLM48_RS20155 WP_179025911.1 4579965..4580888(+) (pilD) [Shewanella sp. Scap07]
ATGAACTCTTTGTTAATACAATTCCAATCCACCATGCTTGAACTACCATGGCTATTCATCTCACTTGCCTTTGTCTTTGC
CGCCACTATCGGTAGCTTTCTCAATGTCGTGATACATCGTTTACCAGTGATGATGAAACGTGAGTGGCAGCAAGAGTGTA
ATCAGTACCTTAATGAGTATCACCAAGAGCTCATTGCCCCGATTGCCAAGAAACTCGGTAAGCCGCTTGATAATTACCCT
GAAAAATATAATTTGGTTGTTCCTGTTTCTGCCTGCCCAAAATGTAAAACCAATATTAAACCTTGGCACAATTTACCTAT
ATTGGGTTGGCTACTGCTACAAGGAAAGTGCGCCAGTTGCGACGCCCCTATTTCAGCCCGTTATCCAGTATTCGAATTGT
TTACAGGAGCTATGGTGGCAACATTGGCTTGGCACTTTGGGCCAAGCTTACAATTTGCAATGGCCGCACTACTCACTTTT
GCTCTAGTCGCGCTAACAGGTATCGATCTAGATGAGATGCTATTGCCTGATCAAATCACTATGCCGCTGTTATGGCTTGG
TTTAATCATCAACATTAATGGCTTGTTCACTCCATTGACCGACGCTTTGATAGGCGCTGTAGCTGGCTACTTAAGCCTGT
GGAGTGTCTACTGGCTTTTTAAAATAGCGACCGGTAAAGAAGGTATGGGGTATGGTGACTTTAAACTCATGGCAGTGTTT
GGCGCTTGGCTAGGTTCGCAAATGTTACCTCTAATCATTCTGCTATCTTCTCTAGTGGGGGCTATCGTCGGCATTATGCT
AATCATTAACAAAAAATTGAATAGTGGTAATCCTATCCCTTTTGGTCCCTATATTGCGGCTGCAGGTTGGATAGCACTCC
TTTGGGGCGAGCCTATCGTCAATGCATACCTTGCTAGTTTGTAG

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(25-146)

Type IV leader peptidase family (Peptidase_A24)

(157-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

52.041

95.765

0.498
  pilD Vibrio campbellii strain DS40M4

50.512

95.44

0.482
  pilD Acinetobacter nosocomialis M2

49.135

94.137

0.463
  pilD Acinetobacter baumannii D1279779

48.789

94.137

0.459
  pilD Neisseria gonorrhoeae MS11

45.956

88.599

0.407

Multiple sequence alignment