Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FKV23_RS13245 Genome accession   NZ_CP041242
Coordinates   2993751..2995472 (+) Length   573 a.a.
NCBI ID   WP_141624272.1    Uniprot ID   A0A514BU75
Organism   Marilutibacter alkalisoli strain SJ-36     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2988751..3000472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FKV23_RS13215 (FKV23_13215) pilR 2989670..2991034 (+) 1365 WP_141624266.1 sigma-54 dependent transcriptional regulator Regulator
  FKV23_RS13220 (FKV23_13220) - 2991148..2991402 (+) 255 WP_208543295.1 antitoxin of toxin-antitoxin stability system -
  FKV23_RS13225 (FKV23_13225) - 2991399..2991689 (+) 291 WP_141624268.1 type II toxin-antitoxin system RelE/ParE family toxin -
  FKV23_RS17840 (FKV23_13230) - 2991742..2992026 (-) 285 WP_407067626.1 addiction module protein -
  FKV23_RS17385 - 2992370..2992469 (+) 100 Protein_2629 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FKV23_RS17845 - 2993076..2993177 (+) 102 Protein_2630 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FKV23_RS13245 (FKV23_13245) pilB 2993751..2995472 (+) 1722 WP_141624272.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FKV23_RS13250 (FKV23_13250) pilC 2995856..2997058 (+) 1203 WP_407067681.1 type II secretion system F family protein Machinery gene
  FKV23_RS13255 (FKV23_13255) pilD 2997068..2997931 (+) 864 WP_141624274.1 A24 family peptidase Machinery gene
  FKV23_RS13260 (FKV23_13260) coaE 2997953..2998603 (+) 651 WP_141624275.1 dephospho-CoA kinase -
  FKV23_RS13265 (FKV23_13265) - 2998633..2999613 (-) 981 WP_141624276.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62228.63 Da        Isoelectric Point: 6.1503

>NTDB_id=370774 FKV23_RS13245 WP_141624272.1 2993751..2995472(+) (pilB) [Marilutibacter alkalisoli strain SJ-36]
MNTVATTNLVGITGIARRLVMDGALDEHAAREAMEAATTEKKPLALYLAEKRLVSQPALAAANSVEFGMPLFDPGALDPT
QSAIRLISEELARKHNVLPLYKRGNRLFIGIADPTNIHALDEIKFQTNLVVDAVLVDDESIRRNLDRWLDSADVLESAAG
DEGLENLEVGNVDAEAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYETEYRVRLRIDGLLKQIAKVPVKLQ
ARIGARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKIVLRILDGGAAKLGIDMLGYESDQKQLYLG
AIAKPYGMVLVTGPTGSGKTVSLYTALNILNDDTRNISTVEDPVEIRVPGINQVQMNTKRGMTFAAALRSFLRQDPDVIM
VGEIRDLETAEIGIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPFNITSSVTLVIAQRLARRLHSCKRKVELPAHALL
AEGFTQEEIDAGLTLYEAVGCPDCTEGYKGRTGIYQVMPMSDEIQAIVLAGGNAMQIAEAAQKAGVRDLRQSALLKVKNG
VTSLAEINRVTKD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=370774 FKV23_RS13245 WP_141624272.1 2993751..2995472(+) (pilB) [Marilutibacter alkalisoli strain SJ-36]
ATGAACACCGTCGCGACCACCAACCTGGTCGGCATCACCGGCATTGCCCGGCGCCTGGTCATGGACGGCGCGCTCGACGA
GCATGCCGCCCGCGAGGCGATGGAAGCGGCCACCACGGAAAAGAAACCGCTGGCGCTGTACCTGGCCGAGAAGCGGCTGG
TGTCGCAACCGGCGCTGGCGGCGGCCAACTCGGTCGAGTTCGGCATGCCGTTGTTCGACCCTGGCGCGCTCGACCCGACG
CAGTCGGCGATCCGGCTGATCAGCGAAGAGCTGGCTCGCAAGCACAACGTGCTGCCGCTGTACAAGCGCGGCAACCGCCT
GTTCATCGGCATCGCCGACCCGACCAACATCCATGCGCTGGACGAGATCAAGTTCCAGACCAACCTGGTGGTGGACGCTG
TGCTGGTCGACGACGAGTCGATCCGGCGCAACCTGGACCGCTGGCTGGACAGCGCCGACGTGCTCGAAAGCGCCGCCGGC
GATGAAGGGCTGGAGAACCTGGAGGTCGGCAATGTCGACGCCGAGGCCGGCGGCGACTCCGGCGTCGACGCCAAGGGTGA
CGACACTCCGGTGGTCAAGTTCGTCAACAAGGTGCTGGTGGACGCGATCCGCCGCGGTGCCTCGGACATCCACTTCGAAC
CCTACGAGACCGAATACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGCAGATCGCCAAGGTCCCGGTCAAGCTGCAG
GCCCGCATCGGCGCGCGCCTGAAGGTAATGGCGCAGCTCGACATCGCCGAGAAGCGGGTGCCGCAGGACGGGCGAATCAA
GCTCAACCTGAGCAAGACCAAGCAGATCGACTTCCGAGTCAGCACCCTGCCCACCCTTTTTGGCGAGAAGATCGTGCTGC
GTATCCTCGATGGCGGCGCGGCCAAGCTCGGCATCGACATGCTCGGTTACGAGAGCGACCAGAAGCAGCTGTACCTGGGC
GCCATCGCCAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGCTCAGGCAAGACGGTGTCGCTGTACACCGCGCT
GAACATCCTCAACGACGACACCCGCAACATATCCACCGTCGAAGACCCGGTCGAGATCCGCGTGCCGGGCATCAACCAGG
TGCAGATGAACACCAAGCGCGGGATGACCTTCGCCGCCGCGCTGCGCAGTTTCCTGCGCCAGGACCCGGACGTGATCATG
GTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGGCATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTCGACCCT
GCACACCAACGACGCGCCGCAGACCATCGCCCGGCTGATGAACATGGGCGTGGCGCCGTTCAACATCACCAGTTCGGTGA
CCCTGGTCATCGCCCAGCGCCTGGCCCGGCGCCTGCACAGCTGCAAGCGCAAGGTGGAGCTGCCCGCGCATGCACTGCTG
GCCGAGGGCTTCACCCAGGAAGAGATCGACGCCGGCCTGACCCTGTACGAGGCGGTTGGCTGCCCGGACTGCACCGAGGG
CTACAAGGGCCGCACCGGCATCTACCAGGTCATGCCGATGAGCGACGAGATCCAGGCCATCGTGCTGGCCGGCGGCAACG
CGATGCAGATCGCCGAGGCGGCGCAGAAGGCCGGTGTCCGCGACCTGCGCCAGTCGGCACTGCTGAAGGTCAAGAACGGG
GTGACCAGTCTGGCCGAGATCAATCGGGTGACGAAGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A514BU75

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.276

100

0.543
  pilB Acinetobacter baumannii D1279779

54.24

98.778

0.536
  pilB Legionella pneumophila strain ERS1305867

50.617

98.953

0.501
  pilF Neisseria gonorrhoeae MS11

47.627

99.302

0.473
  pilB Vibrio cholerae strain A1552

52.446

89.18

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

52.147

85.34

0.445
  pilB Vibrio campbellii strain DS40M4

51.318

86.038

0.442
  pilF Thermus thermophilus HB27

40.812

90.227

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.771

91.274

0.363

Multiple sequence alignment