Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   FKV23_RS13255 Genome accession   NZ_CP041242
Coordinates   2997068..2997931 (+) Length   287 a.a.
NCBI ID   WP_141624274.1    Uniprot ID   A0A514BU96
Organism   Marilutibacter alkalisoli strain SJ-36     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2992068..3002931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FKV23_RS17385 - 2992370..2992469 (+) 100 Protein_2629 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FKV23_RS17845 - 2993076..2993177 (+) 102 Protein_2630 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FKV23_RS13245 (FKV23_13245) pilB 2993751..2995472 (+) 1722 WP_141624272.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FKV23_RS13250 (FKV23_13250) pilC 2995856..2997058 (+) 1203 WP_407067681.1 type II secretion system F family protein Machinery gene
  FKV23_RS13255 (FKV23_13255) pilD 2997068..2997931 (+) 864 WP_141624274.1 A24 family peptidase Machinery gene
  FKV23_RS13260 (FKV23_13260) coaE 2997953..2998603 (+) 651 WP_141624275.1 dephospho-CoA kinase -
  FKV23_RS13265 (FKV23_13265) - 2998633..2999613 (-) 981 WP_141624276.1 Nudix family hydrolase -
  FKV23_RS13270 (FKV23_13270) secA 2999749..3002478 (-) 2730 WP_141624277.1 preprotein translocase subunit SecA -

Sequence

Protein


Download         Length: 287 a.a.        Molecular weight: 31793.54 Da        Isoelectric Point: 7.2394

>NTDB_id=370776 FKV23_RS13255 WP_141624274.1 2997068..2997931(+) (pilD) [Marilutibacter alkalisoli strain SJ-36]
MAFLDQNPAIGYPLVAGLGLLVGSFLNVVILRLPKRLEWEWQRDAREMLDEPDGYDPPPPGIVVERSHCPHCKHQLSWYE
NIPVFSWLALRGKCRNCKAPISPQYPLVEMLTMLLALVSVWQFGFGWQGFGAALFSCFLIASSGIDFRTQLLPDQLTLPL
MWLGLIAAADNLYMPAKPALLGAIAGYLSLWSVWWVFKQLTGKEGMGHGDFKLLAAIGAWVGLSGVLPTIMLSSLIGAIV
GSIWLFAKGRDRATPIPFGPYLAIAGWIVFFWGEGMIDWYLRISGLA

Nucleotide


Download         Length: 864 bp        

>NTDB_id=370776 FKV23_RS13255 WP_141624274.1 2997068..2997931(+) (pilD) [Marilutibacter alkalisoli strain SJ-36]
ATGGCATTCCTAGACCAGAACCCCGCCATCGGCTATCCGCTGGTGGCGGGCCTCGGGCTGCTGGTGGGCAGCTTCCTCAA
CGTGGTGATCCTGCGCCTGCCCAAGCGGCTGGAGTGGGAGTGGCAGCGCGACGCGCGCGAGATGCTGGACGAGCCCGACG
GCTACGACCCGCCACCGCCCGGCATCGTGGTGGAGCGCTCGCACTGTCCGCACTGCAAGCACCAACTGAGCTGGTACGAG
AACATCCCGGTGTTCAGTTGGCTGGCCCTGCGCGGCAAATGCCGGAACTGCAAGGCACCGATCTCGCCGCAGTATCCGCT
GGTGGAGATGCTGACCATGTTGCTGGCGCTGGTCAGCGTGTGGCAGTTCGGTTTCGGCTGGCAGGGCTTCGGCGCGGCGT
TGTTCTCGTGCTTCCTGATCGCATCGTCGGGCATCGACTTCCGCACCCAGTTGCTGCCCGACCAGCTCACCCTGCCGCTG
ATGTGGCTGGGGTTGATCGCCGCAGCCGACAACCTCTACATGCCGGCCAAGCCGGCCCTACTCGGCGCGATCGCCGGCTA
CCTTAGCCTGTGGTCGGTGTGGTGGGTGTTCAAGCAACTCACCGGCAAGGAAGGCATGGGCCATGGCGATTTCAAGCTGC
TGGCCGCGATCGGCGCCTGGGTGGGCCTGAGCGGCGTGCTGCCGACGATCATGCTGTCGTCGCTGATCGGCGCCATCGTC
GGCTCGATCTGGCTGTTCGCCAAAGGCCGCGACCGCGCCACCCCGATCCCGTTTGGCCCTTATCTGGCGATCGCCGGCTG
GATCGTGTTCTTCTGGGGCGAGGGGATGATCGACTGGTATCTGCGGATTTCGGGCCTGGCATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-123)

Type IV leader peptidase family (Peptidase_A24)

(134-241)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A514BU96

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

52.364

95.819

0.502
  pilD Acinetobacter baumannii D1279779

47.917

100

0.481
  pilD Vibrio campbellii strain DS40M4

49.813

93.031

0.463
  pilD Acinetobacter nosocomialis M2

47.163

98.258

0.463
  pilD Neisseria gonorrhoeae MS11

46.52

95.122

0.443

Multiple sequence alignment