Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   FKZ69_RS04450 Genome accession   NZ_CP041236
Coordinates   952234..953442 (-) Length   402 a.a.
NCBI ID   WP_141605477.1    Uniprot ID   -
Organism   Pseudomonas azotoformans strain P45A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 947234..958442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FKZ69_RS04410 (FKZ69_04410) - 948151..948324 (-) 174 WP_016976295.1 DUF3094 family protein -
  FKZ69_RS04415 (FKZ69_04415) - 948378..949007 (-) 630 WP_027604891.1 DUF1780 domain-containing protein -
  FKZ69_RS04420 (FKZ69_04420) - 949113..949580 (+) 468 WP_092489006.1 FAD/FMN-containing dehydrogenase -
  FKZ69_RS04425 (FKZ69_04425) - 949577..950266 (+) 690 WP_141605475.1 energy-coupling factor ABC transporter permease -
  FKZ69_RS04430 (FKZ69_04430) - 950342..950557 (+) 216 WP_003171680.1 hypothetical protein -
  FKZ69_RS04435 (FKZ69_04435) yacG 950554..950754 (-) 201 WP_027604888.1 DNA gyrase inhibitor YacG -
  FKZ69_RS04440 (FKZ69_04440) coaE 950751..951374 (-) 624 WP_092489004.1 dephospho-CoA kinase -
  FKZ69_RS04445 (FKZ69_04445) pilD 951371..952237 (-) 867 WP_141605476.1 prepilin peptidase Machinery gene
  FKZ69_RS04450 (FKZ69_04450) pilC 952234..953442 (-) 1209 WP_141605477.1 type II secretion system F family protein Machinery gene
  FKZ69_RS04455 (FKZ69_04455) - 953645..954055 (+) 411 WP_092489084.1 pilin -
  FKZ69_RS04460 (FKZ69_04460) - 954091..955491 (+) 1401 WP_141605478.1 O-antigen ligase family protein -
  FKZ69_RS32890 (FKZ69_04470) - 956407..956748 (+) 342 WP_186296024.1 helix-turn-helix domain-containing protein -
  FKZ69_RS32455 (FKZ69_04475) - 956752..957936 (+) 1185 WP_222104850.1 replication protein RepA -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43286.53 Da        Isoelectric Point: 10.1915

>NTDB_id=370718 FKZ69_RS04450 WP_141605477.1 952234..953442(-) (pilC) [Pseudomonas azotoformans strain P45A]
MDNASTTYAWEGINRKGRRVSGQTKGHNAALVKAQLRQQGISPGRVRKQSPLLPSLTPSVKATDITLFTRQLATLLKAGV
PLLQAFDIISEGVDKRAVREQVLGLKQAIAAGSSLAEALRKQPRYFDKLYCNLVAAGEQAGALETLLERVAIHREKSEQL
KARIKKAMTYPIAVLVVASLVTGILLVHVVPQFQALFAGVDGKLPAFTLHVIALSAFMQHAWWMLVLGIVAGAAGLRQAY
RASSSFRYWLDTGLLKAPLAGKLLTKSAVARYARTLSTTFAAGVPLVQALDSVAGAVGNDPFKQAIEHMRHDVSNGMQLN
QSMLNSGLFPGMAIQLTAIGEESGTLEHMLEKVANHYEADVDNQVDNLTSLMEPLIMVVLGGIVGALVIAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=370718 FKZ69_RS04450 WP_141605477.1 952234..953442(-) (pilC) [Pseudomonas azotoformans strain P45A]
ATGGACAACGCTTCAACGACTTACGCCTGGGAAGGCATCAACCGCAAAGGGCGCAGGGTGTCCGGGCAAACCAAGGGGCA
CAACGCAGCCCTGGTCAAGGCACAACTGCGCCAGCAAGGGATCAGCCCCGGTCGTGTGCGCAAGCAATCCCCGCTGCTGC
CAAGCCTCACCCCTTCAGTCAAAGCGACTGACATCACGCTGTTCACGCGTCAACTGGCTACGCTGCTGAAGGCTGGGGTT
CCACTCCTGCAAGCCTTCGACATCATCAGCGAAGGCGTCGACAAACGCGCTGTGCGTGAGCAGGTGCTGGGACTTAAACA
GGCGATCGCCGCCGGCAGCAGCCTGGCTGAGGCATTACGCAAACAACCGCGTTATTTCGATAAGCTGTACTGCAACCTGG
TGGCCGCCGGCGAACAGGCCGGGGCGCTTGAGACACTGCTGGAAAGGGTGGCGATCCACCGGGAAAAGAGTGAACAGCTC
AAGGCCAGGATCAAAAAGGCCATGACCTACCCCATTGCTGTCCTGGTGGTTGCCAGCCTGGTCACCGGCATACTGCTGGT
CCACGTGGTACCGCAGTTCCAGGCCCTGTTCGCCGGAGTCGACGGCAAACTGCCGGCATTTACCTTGCACGTGATCGCCC
TGTCGGCGTTCATGCAGCACGCATGGTGGATGCTGGTATTGGGCATAGTCGCGGGAGCCGCTGGGCTGCGCCAGGCCTAT
CGGGCTTCGTCGAGCTTTCGCTACTGGCTGGACACCGGTTTGTTGAAAGCGCCCCTGGCAGGCAAACTGCTGACAAAATC
CGCCGTGGCCCGCTACGCCCGCACGCTCTCCACGACCTTCGCCGCCGGTGTTCCACTGGTCCAGGCATTGGATTCAGTCG
CCGGCGCCGTGGGAAACGACCCGTTCAAACAGGCAATCGAACATATGCGTCACGATGTATCCAACGGCATGCAACTGAAT
CAATCCATGCTCAACAGCGGCCTGTTTCCCGGCATGGCAATCCAACTGACGGCGATCGGCGAAGAGTCGGGCACCCTGGA
ACACATGCTGGAAAAGGTCGCCAACCACTATGAGGCTGACGTGGACAACCAGGTCGACAACCTCACCAGCCTGATGGAGC
CATTGATCATGGTGGTGCTGGGGGGCATCGTCGGCGCGCTGGTCATCGCCATGTACCTGCCGGTCTTCCAGCTGGGGAGT
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

60.453

98.756

0.597
  pilC Acinetobacter baumannii D1279779

53.788

98.507

0.53
  pilC Acinetobacter baylyi ADP1

53.418

98.259

0.525
  pilC Legionella pneumophila strain ERS1305867

48.768

100

0.493
  pilC Vibrio cholerae strain A1552

41.646

99.751

0.415
  pilC Vibrio campbellii strain DS40M4

40.355

98.01

0.396
  pilG Neisseria gonorrhoeae MS11

38.845

94.776

0.368
  pilG Neisseria meningitidis 44/76-A

38.32

94.776

0.363

Multiple sequence alignment