Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FGA12_RS16870 Genome accession   NZ_CP041153
Coordinates   3838840..3840549 (+) Length   569 a.a.
NCBI ID   WP_033540802.1    Uniprot ID   -
Organism   Shewanella marisflavi strain ECSMB14101     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3833840..3845549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGA12_RS16850 (FGA12_16850) ampE 3834692..3835543 (-) 852 WP_033540799.1 beta-lactamase regulator AmpE -
  FGA12_RS16855 (FGA12_16855) ampD 3835575..3836126 (-) 552 WP_033540800.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FGA12_RS16860 (FGA12_16860) nadC 3836405..3837286 (+) 882 WP_033540801.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FGA12_RS19480 (FGA12_16865) - 3838296..3838730 (+) 435 WP_309147283.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FGA12_RS16870 (FGA12_16870) pilB 3838840..3840549 (+) 1710 WP_033540802.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FGA12_RS16875 (FGA12_16875) pilC 3840655..3841920 (+) 1266 WP_033540803.1 type II secretion system F family protein Machinery gene
  FGA12_RS16880 (FGA12_16880) pilD 3841969..3842880 (+) 912 WP_033540804.1 A24 family peptidase Machinery gene
  FGA12_RS16885 (FGA12_16885) coaE 3842905..3843510 (+) 606 WP_033540805.1 dephospho-CoA kinase -
  FGA12_RS16890 (FGA12_16890) zapD 3843519..3844253 (+) 735 WP_033540806.1 cell division protein ZapD -
  FGA12_RS16895 (FGA12_16895) yacG 3844302..3844514 (+) 213 WP_033540807.1 DNA gyrase inhibitor YacG -
  FGA12_RS16900 (FGA12_16900) mutT 3844515..3844904 (+) 390 WP_033540808.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63188.33 Da        Isoelectric Point: 4.9978

>NTDB_id=369956 FGA12_RS16870 WP_033540802.1 3838840..3840549(+) (pilB) [Shewanella marisflavi strain ECSMB14101]
MPTTGLHLGLSTLFIRKQLLSEEQIASAITKSRQSKISLVSTIVSEKLLSAREIAELCYEEYGTPLLDLNEFDISSIPED
FLNKKLIEKHKCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEDDKLTLALEKVLEEDISALDLEGIDEDS
LSGIEITDTDKRQEEQQGDASDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLAGRISA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRKLYEEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCTECRQPEEVPEHELLRLGF
TQTQIDEGFTVYKPVGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIANQAKEQGMRDLRDSGLRKVIAGVTSI
AEINRVTSF

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=369956 FGA12_RS16870 WP_033540802.1 3838840..3840549(+) (pilB) [Shewanella marisflavi strain ECSMB14101]
ATGCCAACAACAGGTCTCCACCTTGGATTATCAACGCTCTTTATTCGCAAACAACTTCTTAGCGAAGAGCAGATCGCATC
AGCTATAACTAAGTCGCGTCAATCGAAAATCTCCCTAGTATCTACTATCGTTTCCGAAAAACTGCTCTCCGCAAGAGAAA
TAGCTGAACTCTGCTACGAAGAATACGGTACCCCTCTGCTGGATCTCAATGAGTTCGATATCAGTAGCATTCCAGAAGAT
TTTCTCAACAAGAAGCTGATCGAAAAGCACAAATGCTTACCATTATTTAAACGCGGCAACCGTCTCTATATTGGCACCTC
AGACCCCACCAATATCGCCGCCTTAGAAGACTTTCAATTTAGTGCGGGCCTGCACGCCGAAGCCATACTGGTTGAAGATG
ACAAGCTAACCTTAGCGCTTGAGAAGGTACTCGAAGAAGATATCTCGGCACTCGATCTCGAAGGTATAGATGAAGACTCA
CTTTCCGGCATAGAGATCACCGACACTGACAAACGCCAGGAAGAGCAGCAAGGCGATGCCAGTGATGACGCCCCCATTGT
TATCTATATCAATAAAATTCTCACCGATGCCATACGTAAAGGCGCATCAGACTTACACTTCGAGCCCTACGAAAAGCGCT
ACCGTATTCGTTTTCGTATCGATGGCATCTTACATGAAGTCTCTGAACCTCCGGTGAACTTGGCTGGCCGTATCTCAGCT
CGCCTTAAGGTGATGTCAAAGCTAGATATCGCCGAGCGTCGCGTCCCCCAAGATGGCCGCATCAAGATGAAGCTGTCGCG
CACTAAATCTATCGATTTTCGTGTGAGCACCTTGCCGACAATTTGGGGCGAAAAGATCGTAATGCGTATCTTGGACTCCT
CATCGGCTCAGCTCGGTATCGAAAAACTCGGCTATGAAGATGACCAGCGCAAACTCTATGAAGAGATGCTGGCAAAGCCT
CAGGGCATGATCTTGGTCACTGGCCCTACGGGCTCAGGTAAAACAGTGTCCCTCTATACAGGCCTTAATATTCTTAATAC
CGAAGAGCGCAATATTTCTACCGCAGAAGACCCAGTTGAAATTAACCTAGAAGGCGTCAATCAAGTTCATATTAACTTGA
AAGCAGGCCTTACTTTTGCGTCAGCCCTACGTTCATTCCTCCGCCAAGATCCAGATGTCGTCATGGTGGGTGAGATTCGA
GACCTGGAAACCGCAGAAATCGCCATTAAGGCTGCGCAAACCGGTCACCTAGTACTGTCAACTCTTCACACTAACTCAGC
TGCCGAAACCCTAACCCGCCTGATTAACATGGGCGTGCCTGGTTACAACATCGCCAGCTCGGTTAACCTGATTATCGCCC
AGCGTCTCGCCCGAAGACTCTGCACCGAATGTCGTCAACCCGAAGAAGTGCCCGAGCATGAACTACTCAGATTAGGTTTT
ACCCAAACGCAAATTGATGAAGGCTTTACCGTCTATAAGCCCGTTGGCTGTGACCTCTGCTCCGGCGGTTACAAAGGCCG
AGTGGGTATTTATGAGGTGATGAAGATGTCAGATGAGATTGCCCGTACTATTATGGAAGGAGGCAACTCGCTGCAAATCG
CTAATCAGGCCAAAGAACAAGGCATGCGCGATCTTCGGGACTCGGGCCTACGCAAGGTCATTGCGGGCGTAACCAGTATC
GCCGAAATAAACCGAGTCACTAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.289

98.594

0.575
  pilB Acinetobacter baumannii D1279779

57.041

98.594

0.562
  pilB Legionella pneumophila strain ERS1305867

55.654

99.473

0.554
  pilB Vibrio parahaemolyticus RIMD 2210633

51.494

100

0.515
  pilB Vibrio cholerae strain A1552

51.779

98.77

0.511
  pilB Vibrio campbellii strain DS40M4

51.332

98.946

0.508
  pilF Neisseria gonorrhoeae MS11

47.173

99.473

0.469
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.959

97.891

0.381
  pilF Thermus thermophilus HB27

37.345

99.297

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

39.81

92.267

0.367

Multiple sequence alignment