Detailed information NTG0368464

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: FIU05_RS15300
• Genome accession: NZ_CP040987
• Coordinates: 3351907..3352971 (+)
• Length: 354 a.a.
• NCBI ID: WP_010946662.1
• Uniprot ID: Q5ZX06
• Organism: Legionella pneumophila strain FDAARGOS_779
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3346907..3357971

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FIU05_RS15290 (FIU05_15290)	-	3347944..3350328 (-)	2385	WP_010946660.1	penicillin-binding protein 1A	-
FIU05_RS15295 (FIU05_15295)	ravI	3350480..3351490 (-)	1011	WP_010946661.1	Dot/Icm T4SS effector RavI	-
FIU05_RS15300 (FIU05_15300)	pilM	3351907..3352971 (+)	1065	WP_010946662.1	type IV pilus assembly protein PilM	Machinery gene
FIU05_RS15305 (FIU05_15305)	pilN	3352975..3353523 (+)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
FIU05_RS15310 (FIU05_15310)	pilO	3353537..3354136 (+)	600	WP_014841277.1	type 4a pilus biogenesis protein PilO	Machinery gene
FIU05_RS15315 (FIU05_15315)	pilP	3354133..3354720 (+)	588	WP_010946665.1	pilus assembly protein PilP	Machinery gene
FIU05_RS15320 (FIU05_15320)	pilQ	3354724..3356820 (+)	2097	WP_010946666.1	type IV pilus secretin PilQ	Machinery gene
FIU05_RS15325 (FIU05_15325)	aroK	3357266..3357793 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38732.71 Da
• Isoelectric Point: 5.0367

>NTDB_id=368464 FIU05_RS15300 WP_010946662.1 3351907..3352971(+) (pilM) [Legionella pneumophila strain FDAARGOS_779]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNIIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQSGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=368464 FIU05_RS15300 WP_010946662.1 3351907..3352971(+) (pilM) [Legionella pneumophila strain FDAARGOS_779]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATCCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGCGAAGAAATACCTGCCTCTGCCTTAGAAGGAAATATAA
TTAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATCGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAATCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5ZX06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.435	100	0.994
comM	Acinetobacter nosocomialis M2	42.817	100	0.429
pilM	Acinetobacter baumannii D1279779	42.535	100	0.427
comM	Acinetobacter baylyi ADP1	41.457	100	0.418