Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   GZS06_RS18380 Genome accession   NZ_CP048376
Coordinates   3834673..3835113 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain 38     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 3829673..3840113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZS06_RS18360 (GZS06_18365) aroP 3830671..3832044 (+) 1374 WP_001347572.1 aromatic amino acid transporter AroP -
  GZS06_RS18365 (GZS06_18370) ampE 3832087..3832941 (-) 855 WP_000172000.1 beta-lactamase regulator AmpE -
  GZS06_RS18370 (GZS06_18375) ampD 3832938..3833489 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GZS06_RS18375 (GZS06_18380) nadC 3833577..3834470 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GZS06_RS18380 (GZS06_18385) pilA 3834673..3835113 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  GZS06_RS18385 (GZS06_18390) pilB 3835123..3836508 (+) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  GZS06_RS18390 (GZS06_18395) hofC 3836498..3837700 (+) 1203 WP_000157236.1 protein transport protein HofC -
  GZS06_RS18395 (GZS06_18400) guaC 3837735..3838778 (-) 1044 WP_001217338.1 GMP reductase -
  GZS06_RS23300 - 3838934..3838978 (-) 45 WP_120795372.1 protein YacM -
  GZS06_RS18400 (GZS06_18405) coaE 3839003..3839623 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence


Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=368463 GZS06_RS18380 WP_000360895.1 3834673..3835113(+) (pilA) [Escherichia coli strain 38]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=368463 GZS06_RS18380 WP_000360895.1 3834673..3835113(+) (pilA) [Escherichia coli strain 38]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363


Multiple sequence alignment