Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FH041_RS18810 Genome accession   NZ_CP040930
Coordinates   4108296..4109561 (+) Length   421 a.a.
NCBI ID   WP_042138980.1    Uniprot ID   A0AAW4D0J0
Organism   Pseudomonas sp. SWI7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4103296..4114561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FH041_RS18790 (FH041_18790) - 4103319..4105769 (-) 2451 WP_027913506.1 penicillin-binding protein 1A -
  FH041_RS18795 (FH041_18795) pilM 4105945..4106826 (+) 882 WP_140174944.1 type IV pilus biogenesis protein PilM -
  FH041_RS18800 (FH041_18800) - 4106823..4107359 (+) 537 WP_110606387.1 PilN domain-containing protein -
  FH041_RS18805 (FH041_18805) - 4107356..4108372 (+) 1017 WP_140174946.1 pilus assembly protein PilP -
  FH041_RS18810 (FH041_18810) pilQ 4108296..4109561 (+) 1266 WP_042138980.1 type IV pilus secretin PilQ Machinery gene
  FH041_RS18815 (FH041_18815) aroK 4109566..4110084 (+) 519 WP_027913510.1 shikimate kinase AroK -
  FH041_RS18820 (FH041_18820) aroB 4110184..4111281 (+) 1098 WP_027913511.1 3-dehydroquinate synthase -
  FH041_RS18825 (FH041_18825) - 4111297..4112889 (+) 1593 WP_027913512.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46034.58 Da        Isoelectric Point: 5.0888

>NTDB_id=368383 FH041_RS18810 WP_042138980.1 4108296..4109561(+) (pilQ) [Pseudomonas sp. SWI7]
MSKTWKWLLATLLLPLTAMAAPYQGERLSLNFQDVEVRAVLQVLADHAGINLVASDDVQGSVTLRLEDVPWDQAIDLVLR
SKGLGRRHEGNVLLIAPLSTLSAQSFEGTSNPLDTQSQPMRRELMRIHYAKAADLSELLLASLSEDGILAGRGSLGVDER
TNTLVVHQPANRLAEIRQLIAQLDVPVRQVAIEARIVEANVDYQKGLGVRWGGDLYSAAPRAGKDLFVDLGVERGGSSLG
VGLLRGDVLLDLELSAMEKSGEGEIISQPKVVTADKETARILKGTEVPYQQSNKNGATTVSFREASLSLEVTPQITPDDS
VVMTVRVTKDEPDYVNALNDVPPIRKNEVNAKVRVENGQTIVIGGVYSTTQNNVVDKVPFFGDLPYVGRLFRRDVLQEKK
SELLVFLTPRIMSDQTIAVSR

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=368383 FH041_RS18810 WP_042138980.1 4108296..4109561(+) (pilQ) [Pseudomonas sp. SWI7]
ATGAGCAAGACATGGAAATGGCTATTGGCAACCCTGCTGCTGCCGTTGACGGCAATGGCGGCCCCTTACCAGGGTGAGCG
GCTGTCACTGAATTTTCAGGATGTCGAGGTACGCGCCGTGCTGCAGGTGCTTGCCGATCATGCCGGCATCAACCTGGTCG
CCAGCGACGACGTACAAGGGAGCGTCACCTTGCGACTGGAAGATGTGCCTTGGGATCAAGCGATCGACCTGGTATTGCGC
AGCAAGGGCCTGGGTCGACGCCACGAAGGCAACGTGCTGCTGATCGCACCGCTTTCTACCCTGAGCGCGCAGTCGTTCGA
GGGCACCTCGAACCCACTCGACACGCAATCACAACCCATGCGTCGGGAACTGATGCGTATCCACTACGCCAAGGCCGCCG
ATTTGTCCGAGCTGTTACTGGCGAGCCTGTCTGAGGACGGCATTCTTGCCGGCAGGGGCAGCCTGGGCGTGGATGAGCGC
ACCAACACGCTGGTGGTCCACCAGCCGGCGAATCGACTCGCCGAGATTCGGCAACTGATCGCCCAATTGGATGTGCCCGT
GCGTCAGGTAGCGATCGAGGCCCGTATCGTGGAGGCCAACGTCGACTACCAGAAAGGCCTGGGGGTGAGATGGGGCGGCG
ACCTGTACAGCGCCGCACCGAGAGCGGGCAAGGACCTGTTCGTTGACCTGGGCGTCGAGCGGGGCGGCAGCAGCCTTGGC
GTTGGTCTGTTGCGCGGCGACGTGCTCCTTGACCTTGAGCTCAGTGCCATGGAAAAGAGCGGTGAGGGCGAGATCATCTC
CCAGCCCAAGGTAGTGACCGCCGACAAGGAGACGGCGCGGATACTCAAAGGTACCGAGGTGCCTTACCAGCAGTCCAACA
AGAATGGCGCGACAACCGTGTCGTTTCGCGAAGCCTCGCTTTCACTGGAGGTGACGCCGCAGATCACGCCGGATGACAGT
GTCGTCATGACCGTGAGAGTGACCAAGGACGAACCTGACTACGTCAATGCCCTCAATGACGTCCCGCCCATTCGCAAGAA
CGAGGTCAATGCCAAGGTCAGGGTCGAGAACGGGCAGACTATCGTGATCGGTGGCGTCTACTCCACCACGCAAAACAATG
TAGTGGACAAGGTGCCATTTTTTGGCGATCTGCCGTATGTTGGACGGCTGTTTCGACGCGATGTACTACAAGAGAAAAAA
TCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGACGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(125-189)

Bacterial type II and III secretion system protein (Secretin)

(256-412)

Secretin and TonB N terminus short domain (STN)

(51-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

54.773

100

0.572
  comQ Acinetobacter baylyi ADP1

41.871

100

0.447
  pilQ Acinetobacter baumannii strain A118

41.048

100

0.447
  pilQ Acinetobacter baumannii D1279779

41.048

100

0.447
  pilQ Legionella pneumophila strain ERS1305867

43.062

99.287

0.428
  pilQ Legionella pneumophila strain Lp02

42.584

99.287

0.423
  pilQ Vibrio campbellii strain DS40M4

39.906

100

0.404
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.625

98.812

0.401
  pilQ Vibrio cholerae strain A1552

40.625

98.812

0.401

Multiple sequence alignment