Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NHAL_RS18655 Genome accession   NC_013960
Coordinates   3965929..3967644 (+) Length   571 a.a.
NCBI ID   WP_041355203.1    Uniprot ID   -
Organism   Nitrosococcus halophilus Nc 4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3960929..3972644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHAL_RS18630 (Nhal_3841) xerC 3961077..3961979 (-) 903 WP_013034702.1 tyrosine recombinase XerC -
  NHAL_RS18635 (Nhal_3842) - 3961986..3962717 (-) 732 WP_013034703.1 DUF484 family protein -
  NHAL_RS18640 (Nhal_3843) dapF 3962714..3963544 (-) 831 WP_013034704.1 diaminopimelate epimerase -
  NHAL_RS18645 (Nhal_3844) - 3963576..3964262 (-) 687 WP_013034705.1 class I SAM-dependent methyltransferase -
  NHAL_RS18650 (Nhal_3845) lysA 3964323..3965573 (-) 1251 WP_013034706.1 diaminopimelate decarboxylase -
  NHAL_RS20750 (Nhal_3846) lptM 3965577..3965711 (-) 135 WP_013034707.1 LPS translocon maturation chaperone LptM -
  NHAL_RS18655 (Nhal_3847) pilB 3965929..3967644 (+) 1716 WP_041355203.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NHAL_RS18660 (Nhal_3848) pilC 3967649..3968869 (+) 1221 WP_013034709.1 type II secretion system F family protein Machinery gene
  NHAL_RS18665 (Nhal_3849) pilD 3968889..3969758 (+) 870 WP_013034710.1 prepilin peptidase Machinery gene
  NHAL_RS18670 (Nhal_3850) coaE 3969808..3970407 (+) 600 WP_013034711.1 dephospho-CoA kinase -
  NHAL_RS18675 (Nhal_3851) zapD 3970503..3971270 (+) 768 WP_013034712.1 cell division protein ZapD -
  NHAL_RS18680 (Nhal_3852) - 3971267..3971455 (+) 189 WP_013034713.1 DNA gyrase inhibitor YacG -
  NHAL_RS18685 (Nhal_3853) - 3971574..3972530 (-) 957 WP_013034714.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62781.96 Da        Isoelectric Point: 5.0129

>NTDB_id=36773 NHAL_RS18655 WP_041355203.1 3965929..3967644(+) (pilB) [Nitrosococcus halophilus Nc 4]
MATPGAQIIMNGLARRLVEDGLLNEADALQAHEQSRKASIPFVTYLVQQKLLKSLDISQAASQEFGVPLFDLDALDLDCL
PKGLVEEKLIRQHQALPIFKRGNRLFVAVADPTNLQALDEIKFHAGINTEAILVEEDKLTRTIDRAMEAQDTSLADLNDT
ALDDLDISGGEEEAPENSIESDVDDTPVVRFINKALLDAIHQGASDIHFEPYEKIYRIRYRQDGVLREVVCPPVSLANRL
AARLKVMARLDISERRVPQDGRMKMSISKNRAIDFRVNTCPTLFGEKIVLRILDPSSAQLGIDALGYEEKQKQLFLETIH
RPYGMVLVTGPTGSGKTVSLYTALNILNTADRNISTAEDPAEINLPGINQVNVYPKVGLTFAGALRAFLRQDPDIIMVGE
IRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTLTRLLNMGVASYNIASAVSLIIAQRLARRLCSQCKAPEKIPQEALLEE
GFTQAQLDTSPTIYKAVGCERCSGGYKGRVGIYQVMPVSEEMGRIIMAGGNSMELSEQAKKEGVADLRQSGLKKVIDGIT
SLEEINRVTKE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=36773 NHAL_RS18655 WP_041355203.1 3965929..3967644(+) (pilB) [Nitrosococcus halophilus Nc 4]
ATGGCAACCCCTGGAGCACAGATCATAATGAACGGTCTGGCCCGCCGTTTAGTGGAAGATGGGCTACTTAACGAAGCGGA
TGCCCTGCAGGCTCATGAGCAGTCCCGCAAGGCCAGCATTCCCTTTGTCACTTACTTAGTCCAGCAAAAACTACTCAAAA
GCCTGGATATCAGCCAAGCGGCCTCACAAGAATTTGGCGTCCCCCTATTCGATCTTGATGCGTTGGATTTGGACTGCCTT
CCCAAAGGCCTAGTGGAAGAGAAACTGATCCGCCAGCACCAAGCATTACCCATCTTCAAGCGCGGGAATCGTCTGTTCGT
TGCCGTGGCCGATCCTACCAACCTCCAGGCATTAGACGAAATTAAATTCCACGCGGGGATTAACACCGAAGCCATCCTCG
TTGAAGAAGACAAACTAACCCGAACCATTGACCGGGCCATGGAGGCCCAGGACACGTCCCTTGCCGATCTAAATGATACC
GCGTTGGATGATCTCGATATTTCCGGGGGCGAAGAGGAGGCCCCGGAAAATTCTATCGAATCTGATGTCGATGATACACC
TGTTGTACGCTTTATTAATAAGGCCCTGTTGGATGCCATTCATCAGGGGGCCTCGGATATTCACTTCGAGCCCTATGAGA
AAATCTATCGGATTAGATACCGACAAGATGGGGTGCTGCGGGAAGTCGTTTGCCCTCCCGTAAGCCTAGCAAACCGGCTA
GCGGCTCGCCTTAAGGTCATGGCCCGCTTGGATATTTCCGAACGGCGCGTGCCCCAAGATGGGCGCATGAAAATGAGTAT
CTCCAAAAACCGAGCAATTGACTTTCGTGTCAATACCTGTCCCACTTTATTTGGTGAAAAAATCGTCCTTCGTATTCTGG
ACCCCTCCAGCGCCCAGTTGGGAATCGACGCCCTCGGTTATGAGGAAAAACAAAAACAGCTCTTTCTAGAAACTATCCAC
CGGCCTTACGGGATGGTGCTGGTTACCGGCCCCACGGGCAGCGGTAAGACCGTCTCCCTTTATACCGCGCTGAATATTCT
GAATACTGCGGACCGCAATATCTCCACCGCCGAAGACCCGGCCGAAATCAACTTGCCCGGCATTAATCAGGTCAACGTAT
ACCCGAAGGTAGGCCTGACTTTTGCTGGAGCCCTAAGAGCCTTTTTACGCCAAGATCCCGATATCATCATGGTCGGTGAG
ATTCGGGATCTGGAAACGGCTGAAATTGCGATTAAAGCAGCCCAAACCGGTCATATGGTGCTCTCCACCCTCCATACCAA
CGACGCCCCCCAGACCCTGACCCGGCTATTAAATATGGGGGTGGCTTCCTATAACATTGCCTCGGCGGTCTCCCTGATCA
TTGCCCAGCGCTTGGCACGGCGCCTTTGTTCCCAATGTAAAGCGCCCGAGAAGATCCCCCAGGAAGCCTTACTAGAAGAA
GGCTTTACCCAAGCCCAGCTCGACACCTCCCCCACTATTTATAAGGCGGTAGGCTGCGAACGCTGCTCAGGAGGCTATAA
GGGCCGAGTGGGCATCTATCAAGTGATGCCCGTATCCGAGGAGATGGGACGCATCATTATGGCAGGGGGCAACTCCATGG
AACTTTCAGAACAGGCCAAAAAAGAAGGCGTGGCCGATCTACGCCAGTCTGGGCTCAAAAAGGTTATTGATGGCATTACC
AGTTTAGAGGAAATTAACCGAGTGACGAAGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.772

99.825

0.587
  pilB Acinetobacter baylyi ADP1

58.099

99.475

0.578
  pilB Legionella pneumophila strain ERS1305867

56.174

100

0.566
  pilF Neisseria gonorrhoeae MS11

52.021

99.65

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

49.117

99.124

0.487
  pilB Vibrio campbellii strain DS40M4

48.587

99.124

0.482
  pilB Vibrio cholerae strain A1552

51.887

92.82

0.482
  pilF Thermus thermophilus HB27

40.18

97.198

0.391
  pilB/pilB1 Synechocystis sp. PCC 6803

41.313

90.718

0.375

Multiple sequence alignment