Detailed information NTG0036775

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NHAL_RS18665
• Genome accession: NC_013960
• Coordinates: 3968889..3969758 (+)
• Length: 289 a.a.
• NCBI ID: WP_013034710.1
• Uniprot ID: D5C3F4
• Organism: Nitrosococcus halophilus Nc 4
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3963889..3974758

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NHAL_RS18650 (Nhal_3845)	lysA	3964323..3965573 (-)	1251	WP_013034706.1	diaminopimelate decarboxylase	-
NHAL_RS20750 (Nhal_3846)	lptM	3965577..3965711 (-)	135	WP_013034707.1	LPS translocon maturation chaperone LptM	-
NHAL_RS18655 (Nhal_3847)	pilB	3965929..3967644 (+)	1716	WP_041355203.1	type IV-A pilus assembly ATPase PilB	Machinery gene
NHAL_RS18660 (Nhal_3848)	pilC	3967649..3968869 (+)	1221	WP_013034709.1	type II secretion system F family protein	Machinery gene
NHAL_RS18665 (Nhal_3849)	pilD	3968889..3969758 (+)	870	WP_013034710.1	prepilin peptidase	Machinery gene
NHAL_RS18670 (Nhal_3850)	coaE	3969808..3970407 (+)	600	WP_013034711.1	dephospho-CoA kinase	-
NHAL_RS18675 (Nhal_3851)	zapD	3970503..3971270 (+)	768	WP_013034712.1	cell division protein ZapD	-
NHAL_RS18680 (Nhal_3852)	-	3971267..3971455 (+)	189	WP_013034713.1	DNA gyrase inhibitor YacG	-
NHAL_RS18685 (Nhal_3853)	-	3971574..3972530 (-)	957	WP_013034714.1	Nudix family hydrolase	-
NHAL_RS18690 (Nhal_3854)	-	3972583..3972783 (+)	201	WP_041355205.1	DUF2905 domain-containing protein	-
NHAL_RS18695 (Nhal_3855)	-	3972857..3973084 (-)	228	WP_013034716.1	zinc-finger domain-containing protein	-
NHAL_RS18700 (Nhal_3856)	nadA	3973149..3974246 (-)	1098	WP_013034717.1	quinolinate synthase NadA	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32311.13 Da
• Isoelectric Point: 6.3252

>NTDB_id=36775 NHAL_RS18665 WP_013034710.1 3968889..3969758(+) (pilD) [Nitrosococcus halophilus Nc 4]
MEVIAFFENHLAAFVASVFLLGLAVGSFLNVVIYRLPLMMERQWEGQCAELHDHPLLEQEPFNLFVPRSHCPACRHKIRA
WENVPLFSYLLLLGRCNHCGAPISPRYPLVELLTGVISAAVAWHFGVSWETIAALLLSYALIALTFIDFDHQLLPDSITL
PFLWFGLGLSLFEVFTDVRTSLIGAMAGYLSLWLVYHLFRLLTKKEGMGYGDFKLLALLGAWLGWTMLPAIILLSSLVGA
ILGTVWLALSSQHRETPLPFGPYLAAAGWLALMWGQDINQFYLSFTGLG

Nucleotide

• Length: 870 bp

>NTDB_id=36775 NHAL_RS18665 WP_013034710.1 3968889..3969758(+) (pilD) [Nitrosococcus halophilus Nc 4]
ATGGAAGTTATTGCCTTTTTTGAGAACCATCTTGCTGCCTTTGTGGCTTCAGTCTTTCTCCTAGGCTTGGCCGTAGGCAG
TTTCCTCAATGTGGTGATTTACCGTCTTCCTCTCATGATGGAAAGACAATGGGAGGGTCAATGCGCAGAACTGCATGACC
ACCCCCTACTGGAGCAGGAACCATTCAACCTGTTCGTCCCCCGCTCTCACTGTCCAGCGTGTCGCCACAAAATCCGAGCT
TGGGAGAACGTTCCCTTATTCAGTTATCTTCTGCTCCTAGGGCGCTGCAATCACTGTGGCGCCCCTATCTCTCCCCGCTA
TCCCCTGGTAGAACTTTTAACCGGCGTGATTTCTGCTGCCGTAGCTTGGCACTTTGGCGTCAGTTGGGAAACCATTGCCG
CTTTGCTTTTAAGCTATGCCCTCATTGCGCTCACCTTTATCGATTTTGACCATCAGCTGCTGCCCGATAGCATTACCCTT
CCTTTTCTTTGGTTTGGGCTAGGACTAAGTTTATTTGAGGTATTCACGGACGTCCGAACCAGCCTAATTGGCGCCATGGC
GGGTTATCTTTCCCTATGGTTGGTTTACCACCTGTTTCGGCTGCTTACCAAAAAAGAGGGGATGGGATATGGCGACTTCA
AACTTTTAGCACTCCTGGGTGCCTGGTTAGGCTGGACGATGCTTCCTGCCATCATTCTACTTTCCTCATTGGTCGGCGCC
ATACTGGGCACTGTATGGCTCGCTCTTTCCAGCCAACACCGAGAAACCCCCCTCCCCTTTGGCCCTTATCTTGCCGCTGC
CGGTTGGTTAGCCTTGATGTGGGGACAGGATATCAACCAGTTTTATCTCTCCTTCACAGGGCTCGGTTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

• Type IV leader peptidase family (Peptidase_A24) (136-243)

3D structure

• AlphaFold DB: D5C3F4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	53.659	99.308	0.533
pilD	Vibrio cholerae strain A1552	55.762	93.08	0.519
pilD	Acinetobacter nosocomialis M2	50.352	98.27	0.495
pilD	Acinetobacter baumannii D1279779	50	98.27	0.491
pilD	Neisseria gonorrhoeae MS11	50.368	94.118	0.474