Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FGM04_RS15935 Genome accession   NZ_CP040717
Coordinates   3429420..3431126 (-) Length   568 a.a.
NCBI ID   WP_005356616.1    Uniprot ID   -
Organism   Aeromonas veronii strain HX3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3424420..3436126
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGM04_RS15905 (FGM04_15925) mutT 3424932..3425336 (+) 405 WP_042053534.1 8-oxo-dGTP diphosphatase MutT -
  FGM04_RS15910 (FGM04_15930) yacG 3425413..3425607 (-) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  FGM04_RS15915 (FGM04_15935) zapD 3425617..3426339 (-) 723 WP_005355104.1 cell division protein ZapD -
  FGM04_RS15920 (FGM04_15940) coaE 3426377..3426991 (-) 615 WP_139478393.1 dephospho-CoA kinase -
  FGM04_RS15925 (FGM04_15945) pilD 3427005..3427877 (-) 873 WP_157161846.1 A24 family peptidase Machinery gene
  FGM04_RS15930 (FGM04_15950) pilC 3427939..3429180 (-) 1242 WP_005355091.1 type II secretion system F family protein Machinery gene
  FGM04_RS15935 (FGM04_15955) pilB 3429420..3431126 (-) 1707 WP_005356616.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  FGM04_RS22440 (FGM04_15960) - 3431129..3431515 (-) 387 WP_100646864.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FGM04_RS15945 (FGM04_15970) nadC 3431831..3432694 (-) 864 WP_064339261.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FGM04_RS15950 (FGM04_15975) - 3432698..3433168 (-) 471 WP_157161847.1 TIGR02281 family clan AA aspartic protease -
  FGM04_RS15955 (FGM04_15980) ampD 3433299..3433874 (+) 576 WP_069554218.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FGM04_RS15960 (FGM04_15985) pdhR 3434153..3434917 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62563.90 Da        Isoelectric Point: 5.3192

>NTDB_id=366776 FGM04_RS15935 WP_005356616.1 3429420..3431126(-) (pilB) [Aeromonas veronii strain HX3]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTTDDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=366776 FGM04_RS15935 WP_005356616.1 3429420..3431126(-) (pilB) [Aeromonas veronii strain HX3]
ATGACCTCTAGTCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGATTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTCCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTACATCGCCATGTCGGA
TCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAAGCGCTGCTGGTAGAGGAAAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAACGACGCCCTCGGCATGGAGGATATCGACGAATCCGAGATT
TCGGATCTCGAGGTCTCCGACGAAAACAGCCGGCTGGACGAGAGCGTCAATACCACCGATGACGATGCCCCTATCGTCAA
GTACATCAACAAAATCCTGATGGATGCCATCAAGCGCGGCGCCTCCGACCTGCACTTCGAGCCTTATGAGTTCAAGTATC
GGGTTCGCTTTCGCATCGATGGCATTCTGCATGAAATTGCCACACCGCCGGTTAATCTCTCCAACCGCTTCTCCGCTCGC
CTCAAGGTGATGGCACGACTCGATATCGCCGAGCGGCGGCTGCCGCAGGATGGCCGCATCAAGTTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCTAAGCCACAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTAAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTTGCAAGCGCCCTGCGCTCCTTCCTGCGGCAAGATCCGGATGTGGTGATGGTGGGTGAAATTCGCGAT
CTTGAAACCGCAGAGATTGCCATCAAGGCGGCGCAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCATTGCAAAGCGCCGGAAGTGGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGCTGGCTGCCGGACTGCGACTGTTCAAACCGGTCGGTTGCAAGGAGTGCTCGGGGGGTTATAAAGGACGGGT
CGGTATCTACGAGATTATGCTGATGTCGGAAAACATTGCAAAACTGATCATGCAGGGTGCCAATTCGCTACAGATCGCTG
CCATAGCTCAGAAAGAGGGGATGCGTACCTTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.827

95.423

0.542
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377

Multiple sequence alignment