Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   TCK1_RS25255 Genome accession   NZ_CP040324
Coordinates   5545151..5546419 (-) Length   422 a.a.
NCBI ID   WP_159266667.1    Uniprot ID   -
Organism   Pseudomonas monteilii strain TCU-CK1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5540151..5551419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TCK1_RS25240 (TCK1_5056) - 5541804..5543417 (-) 1614 WP_027610018.1 SPOR domain-containing protein -
  TCK1_RS25245 (TCK1_5057) aroB 5543433..5544530 (-) 1098 WP_025752278.1 3-dehydroquinate synthase -
  TCK1_RS25250 (TCK1_5058) aroK 5544628..5545146 (-) 519 WP_025752276.1 shikimate kinase AroK -
  TCK1_RS25255 (TCK1_5059) pilQ 5545151..5546419 (-) 1269 WP_159266667.1 type IV pilus secretin PilQ Machinery gene
  TCK1_RS25260 (TCK1_5060) - 5546343..5547344 (-) 1002 WP_025752272.1 pilus assembly protein PilP -
  TCK1_RS25265 (TCK1_5061) - 5547356..5547892 (-) 537 WP_027610016.1 PilN domain-containing protein -
  TCK1_RS25270 (TCK1_5062) pilM 5547889..5548770 (-) 882 WP_019097360.1 type IV pilus biogenesis protein PilM -
  TCK1_RS25275 (TCK1_5063) - 5548962..5551415 (+) 2454 WP_019097361.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45980.69 Da        Isoelectric Point: 4.7088

>NTDB_id=363266 TCK1_RS25255 WP_159266667.1 5545151..5546419(-) (pilQ) [Pseudomonas monteilii strain TCU-CK1]
MKMLMKWVLAALLPIPLASAMPYQGEPLSLNFQDVEVRSVLQVLADYAGVNLVAGDDVQGNVTLRLQDVPWDQALDLVLR
SKGLARRQEGNVLLVAPAAAFAAQPFDAPLGQEPNAQVQPLRRELIPIHHAKAAELVDLLLTSLADDGTLTARGSLAVDE
RTNTLVAYQPADRLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYSENLRPGQELFVDLGVERAGSSL
GLGLLRGDLLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDN
KVIMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=363266 TCK1_RS25255 WP_159266667.1 5545151..5546419(-) (pilQ) [Pseudomonas monteilii strain TCU-CK1]
ATGAAAATGTTGATGAAATGGGTGTTGGCAGCCCTGCTGCCGATCCCGCTGGCGTCGGCGATGCCTTACCAGGGTGAGCC
CCTGTCGTTGAACTTCCAGGATGTGGAAGTGCGCTCGGTGCTGCAGGTGCTGGCTGATTATGCGGGCGTCAACCTGGTTG
CGGGTGACGACGTACAAGGTAACGTCACGTTGCGTTTACAGGATGTACCTTGGGATCAGGCACTCGACCTGGTGTTGCGA
AGCAAGGGCCTGGCCCGGCGGCAGGAGGGTAATGTACTGCTGGTGGCACCTGCGGCAGCGTTTGCCGCGCAGCCGTTCGA
TGCCCCCTTGGGGCAGGAGCCGAATGCTCAGGTGCAGCCGCTACGCCGTGAGCTGATACCGATCCATCATGCCAAGGCCG
CCGAGCTGGTTGATCTATTGCTGACCAGCCTGGCGGATGACGGCACCCTCACTGCCAGAGGCAGCCTGGCTGTCGACGAG
CGCACCAATACACTGGTGGCGTACCAGCCCGCCGACCGCCTGGCGGAACTGCGGCAGCTGGTTGCGCAACTGGACGTGCC
GGTGCGCCAGGTGGCGATCGAGGCGCGTATCGTCGAAGCCAACGTCGATTACGAGAAAAGCCTGGGGGTGCGCTGGGGCG
GGCCGTTGTATAGCGAAAACCTGCGGCCGGGCCAGGAGCTGTTTGTCGACCTTGGTGTGGAGCGTGCGGGCAGTAGCCTC
GGCCTGGGCCTGCTGCGTGGCGACCTGTTGCTCGACCTGGAGCTCAGCGCCATGGAAAAAAGCGGCAACGGCGAGATCAT
CTCGCAGCCCAAAGTGGTAACGGCCGACAAGGAAACGGCCAGGATCCTCAAAGGCACCGAGGTGCCCTACCAGGAAACCA
GTCAAAGTGGCGCCACCTCGGTGTCGTTTCGTGAAGCCTCGCTGTCGCTGGAGGTGACACCGCAAATCACCCCGGACAAC
AAGGTGATCATGACCGTCAGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATTCGCAA
GAACGAGGTCAATGCCAAGGTTCGCGTGGCGGACGGTGAAACCATCGTGATTGGTGGCGTGTACTCCACTTCGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)

Secretin and TonB N terminus short domain (STN)

(50-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.404

100

0.614
  comQ Acinetobacter baylyi ADP1

42.444

100

0.453
  pilQ Acinetobacter baumannii D1279779

41.048

100

0.445
  pilQ Acinetobacter baumannii strain A118

41.048

100

0.445
  pilQ Vibrio campbellii strain DS40M4

40.323

100

0.415
  pilQ Legionella pneumophila strain ERS1305867

41.866

99.052

0.415
  pilQ Legionella pneumophila strain Lp02

41.866

99.052

0.415
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.093

99.763

0.41
  pilQ Vibrio cholerae strain A1552

41.093

99.763

0.41

Multiple sequence alignment