Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FEF05_RS01205 Genome accession   NZ_CP040243
Coordinates   226980..228365 (-) Length   461 a.a.
NCBI ID   WP_149350582.1    Uniprot ID   -
Organism   Glaesserella parasuis strain HPS-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 221980..233365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FEF05_RS01180 (FEF05_01185) clpP 221997..222578 (+) 582 WP_010787003.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  FEF05_RS01185 (FEF05_01190) clpX 222585..223835 (+) 1251 WP_149350580.1 ATP-dependent protease ATP-binding subunit ClpX -
  FEF05_RS01190 (FEF05_01195) dapE 223909..225042 (-) 1134 WP_005711772.1 succinyl-diaminopimelate desuccinylase -
  FEF05_RS01195 (FEF05_01200) - 225126..225794 (-) 669 WP_035491631.1 prepilin peptidase -
  FEF05_RS01200 (FEF05_01205) pilC 225791..226987 (-) 1197 WP_149350581.1 type II secretion system F family protein Machinery gene
  FEF05_RS01205 (FEF05_01210) pilB 226980..228365 (-) 1386 WP_149350582.1 GspE/PulE family protein Machinery gene
  FEF05_RS01210 (FEF05_01215) - 228698..229063 (-) 366 WP_149350583.1 endonuclease domain-containing protein -
  FEF05_RS01215 (FEF05_01220) pilA 229504..229956 (-) 453 WP_149350584.1 pilin Machinery gene
  FEF05_RS01220 (FEF05_01225) radA 230144..231520 (+) 1377 WP_149350585.1 DNA repair protein RadA -
  FEF05_RS01225 (FEF05_01230) gmk 231573..232199 (-) 627 WP_149350586.1 guanylate kinase -
  FEF05_RS01230 (FEF05_01235) - 232268..233240 (-) 973 Protein_246 IS110 family transposase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52780.63 Da        Isoelectric Point: 5.2525

>NTDB_id=362828 FEF05_RS01205 WP_149350582.1 226980..228365(-) (pilB) [Glaesserella parasuis strain HPS-1]
MSQYTICEQQTERIFDISPSRWQKNCEEKQLLLRYLAVPVQETEHKLWLAVDDENNLTACEIFAFMTHKQIEPVVIASDE
LKYLLNALSPEQQPIYEESELAFVEQEQEQLNLNDPIIQLLDNLFKFCLAQNASDIHIEPRKQKLIIRLRIDGVLHLYKS
LSIQLASRLISRIKLLAKLDISELRQPQDGQFSFTTALADTLDFRVSSLPTIYGEKLVLRLQKNKPTSFDFLQLGFNPQQ
QTTLLNALKQPQGLILVTGPTGSGKSITLYSALNYLNQMDKHILTAEDPVEIEIDGLIQTQVNQSINLDFSQLLRTFLRQ
DPDIIMLGEIRDEESAKMALRASQTGHLVLSTLHTNDALLAVERLLQLGIQEYEIQNSLLLVIAQRLVRKLCKKCRGTGC
DECYQGYKGRIGIYQCLSRTAKKFDKQTAYLDFSSLRESAKQKIEQQLTNETEVDRVLGYE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=362828 FEF05_RS01205 WP_149350582.1 226980..228365(-) (pilB) [Glaesserella parasuis strain HPS-1]
ATGTCCCAATACACTATCTGCGAACAACAAACCGAACGCATTTTTGATATATCGCCTAGCCGTTGGCAAAAAAATTGCGA
GGAGAAACAGCTTTTATTGCGTTACCTTGCCGTTCCTGTGCAAGAAACTGAGCATAAATTATGGCTTGCCGTTGATGATG
AAAACAATCTCACCGCTTGCGAAATCTTTGCTTTTATGACCCATAAGCAGATTGAGCCGGTGGTGATTGCTTCTGATGAA
CTGAAATACTTGCTTAACGCTCTCTCGCCTGAACAACAGCCCATTTATGAAGAGAGCGAGCTGGCTTTTGTTGAACAGGA
GCAAGAACAGCTCAACCTAAACGATCCGATTATTCAATTGCTAGATAATCTCTTTAAATTTTGTCTAGCTCAGAATGCGT
CCGACATTCATATTGAGCCACGCAAGCAAAAATTGATTATCCGCTTACGCATTGACGGCGTTTTGCACCTGTATAAAAGC
CTATCTATTCAGCTGGCAAGCCGACTAATTTCACGGATAAAACTACTGGCAAAGCTAGATATTAGCGAATTACGCCAGCC
ACAAGACGGGCAATTTAGTTTTACCACCGCCCTTGCGGATACCCTTGATTTTCGGGTATCTAGCCTGCCGACGATCTACG
GCGAAAAACTGGTATTGCGCCTACAAAAAAACAAACCAACCAGTTTTGATTTTCTGCAACTTGGCTTTAATCCACAACAG
CAGACCACACTACTCAACGCCTTGAAACAACCGCAAGGGCTGATTTTAGTGACAGGACCAACGGGCAGTGGCAAAAGTAT
CACCTTGTATAGTGCCTTAAACTACCTCAACCAAATGGATAAACATATTCTCACCGCCGAAGATCCTGTGGAAATTGAGA
TTGACGGCTTAATTCAAACCCAAGTCAATCAAAGTATTAACCTTGATTTTAGCCAGCTGTTACGCACCTTTCTACGCCAA
GATCCTGACATCATTATGTTAGGCGAAATTCGAGATGAAGAGAGTGCAAAAATGGCATTAAGAGCAAGCCAGACGGGGCA
TTTAGTCCTTTCAACCCTGCATACCAATGACGCACTATTAGCGGTCGAACGTTTGTTACAACTGGGTATTCAAGAGTATG
AAATCCAAAATTCACTACTGTTAGTCATTGCACAACGCCTTGTGCGTAAGCTCTGCAAAAAGTGTCGTGGCACAGGCTGT
GATGAGTGCTATCAAGGCTATAAAGGGCGGATCGGGATTTACCAATGTTTAAGCAGAACGGCAAAAAAATTCGATAAACA
GACCGCTTACTTGGATTTTTCCAGCCTACGAGAGAGTGCTAAACAGAAAATTGAACAACAACTCACCAATGAAACCGAAG
TGGATAGGGTATTAGGTTATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(30-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

98.698

100

0.987
  pilB Haemophilus influenzae Rd KW20

57.428

97.831

0.562
  pilB Haemophilus influenzae 86-028NP

56.798

98.915

0.562
  pilB Vibrio cholerae strain A1552

38.4

100

0.416
  pilB Vibrio parahaemolyticus RIMD 2210633

39.13

100

0.41
  pilB Vibrio campbellii strain DS40M4

38.923

100

0.408
  pilB Acinetobacter baumannii D1279779

36.381

100

0.406
  pilB Acinetobacter baylyi ADP1

36.832

100

0.403
  pilB Legionella pneumophila strain ERS1305867

37.805

100

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.889

100

0.395
  pilF Neisseria gonorrhoeae MS11

36.211

100

0.373
  pilF Thermus thermophilus HB27

35.43

100

0.367

Multiple sequence alignment