Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   FD735_RS00850 Genome accession   NZ_CP040231
Coordinates   149779..150795 (+) Length   338 a.a.
NCBI ID   WP_081102569.1    Uniprot ID   -
Organism   Streptococcus sp. 1643     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 144779..155795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FD735_RS00830 (FD735_00830) - 145202..147019 (+) 1818 WP_061421837.1 acyltransferase family protein -
  FD735_RS00835 (FD735_00835) nagA 147160..148311 (+) 1152 WP_001134437.1 N-acetylglucosamine-6-phosphate deacetylase -
  FD735_RS00840 (FD735_00840) - 148463..148828 (+) 366 WP_061421836.1 DUF1033 family protein -
  FD735_RS00845 (FD735_00845) comGA/cglA/cilD 148890..149831 (+) 942 WP_061421835.1 competence type IV pilus ATPase ComGA Machinery gene
  FD735_RS00850 (FD735_00850) comGB/cglB 149779..150795 (+) 1017 WP_081102569.1 competence type IV pilus assembly protein ComGB Machinery gene
  FD735_RS00855 (FD735_00855) comGC/cglC 150797..151120 (+) 324 WP_061421834.1 competence type IV pilus major pilin ComGC Machinery gene
  FD735_RS00860 (FD735_00860) comGD/cglD 151083..151517 (+) 435 WP_176553041.1 competence type IV pilus minor pilin ComGD Machinery gene
  FD735_RS00865 (FD735_00865) comGE/cglE 151480..151782 (+) 303 WP_139658261.1 competence type IV pilus minor pilin ComGE Machinery gene
  FD735_RS00870 (FD735_00870) comGF/cglF 151745..152206 (+) 462 WP_139658262.1 competence type IV pilus minor pilin ComGF Machinery gene
  FD735_RS00875 (FD735_00875) comGG/cglG 152184..152597 (+) 414 WP_139658263.1 competence type IV pilus minor pilin ComGG Machinery gene
  FD735_RS00880 (FD735_00880) - 152630..153220 (+) 591 WP_176553042.1 class I SAM-dependent methyltransferase -
  FD735_RS00885 (FD735_00885) comYH 153279..154232 (+) 954 WP_139658264.1 class I SAM-dependent methyltransferase Machinery gene
  FD735_RS00890 (FD735_00890) - 154283..155473 (+) 1191 WP_139658265.1 acetate kinase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38313.40 Da        Isoelectric Point: 9.8696

>NTDB_id=362613 FD735_RS00850 WP_081102569.1 149779..150795(+) (comGB/cglB) [Streptococcus sp. 1643]
MDISQVFRLKRKKLATAKQKKIITLFNNLFSSGFHLVEIISFLGRSALLEKDYVAQMHQGLAQGKSFSEMMNSLGFSSAI
VTQLSLAEAHGNLHLSLGKIEEYLDNLSKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGLVLVCSLSLLLALTFYKRSSKMRVFSMLARIPFLGIFVQTYLTAYYAREWGNMISQGMGLTQIFQIMQEQGSQLFKEI
GQDLAQALQNGRGFSQTIGTYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=362613 FD735_RS00850 WP_081102569.1 149779..150795(+) (comGB/cglB) [Streptococcus sp. 1643]
ATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAGAAGAAAATCATCACCTTGTTTAA
CAACCTCTTCTCCAGTGGCTTTCATTTGGTGGAAATTATTTCTTTCTTGGGAAGAAGTGCCCTGCTAGAAAAGGACTATG
TGGCCCAGATGCACCAAGGCTTGGCTCAGGGGAAATCATTCTCAGAAATGATGAACAGCTTGGGCTTTTCAAGTGCTATT
GTGACCCAGTTATCTCTAGCTGAAGCCCATGGAAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGATAATTT
GTCCAAGGTCAAGAAGAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTACTGGGATTTCTCCTGCTAATCATGTTGG
GGCTCAGGAATTATTTGCTCCCCCAACTGGACAGTAGCAATATTGCCACTCAAATCATCGGCAATCTGCCACAAATATTT
CTGGGACTAGTGCTGGTTTGCTCTCTATCTTTACTTTTAGCCCTCACTTTTTACAAAAGAAGTTCCAAGATGCGGGTCTT
CTCGATGTTAGCGCGGATTCCCTTTCTAGGAATCTTTGTCCAGACCTATCTGACGGCCTATTACGCGCGTGAATGGGGCA
ATATGATTTCACAGGGGATGGGGCTGACGCAGATTTTTCAGATCATGCAGGAACAGGGTTCTCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAGCCCTGCAAAATGGTCGCGGATTTTCTCAGACTATAGGAACCTATCCTTTCTTTAAAAAGGA
GTTGAGTCTCATCATCGAGTATGGAGAAGTCAAGTCCAAGCTAGGGAGTGAGTTGGAAATCTATGCAGAAAAGACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAACTTAGTACAGCCACTGGTTTTTATCTTTGTGGCCCTGATTATCGTT
TTACTTTATGCGGCAATGCTTATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis SK321

92.308

100

0.923
  comGB/cglB Streptococcus mitis NCTC 12261

92.012

100

0.92
  comGB/cglB Streptococcus pneumoniae Rx1

90.237

100

0.902
  comGB/cglB Streptococcus pneumoniae D39

90.237

100

0.902
  comGB/cglB Streptococcus pneumoniae R6

90.237

100

0.902
  comGB/cglB Streptococcus pneumoniae TIGR4

90.237

100

0.902
  comYB Streptococcus gordonii str. Challis substr. CH1

72.917

99.408

0.725
  comYB Streptococcus mutans UA140

58.307

94.379

0.55
  comYB Streptococcus mutans UA159

58.307

94.379

0.55
  comGB Lactococcus lactis subsp. cremoris KW2

51.183

100

0.512

Multiple sequence alignment