Detailed information NTG0362617

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: FD735_RS00870
• Genome accession: NZ_CP040231
• Coordinates: 151745..152206 (+)
• Length: 153 a.a.
• NCBI ID: WP_139658262.1
• Uniprot ID: A0A5B7Y3I0
• Organism: Streptococcus sp. 1643
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 146745..157206

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FD735_RS00835 (FD735_00835)	nagA	147160..148311 (+)	1152	WP_001134437.1	N-acetylglucosamine-6-phosphate deacetylase	-
FD735_RS00840 (FD735_00840)	-	148463..148828 (+)	366	WP_061421836.1	DUF1033 family protein	-
FD735_RS00845 (FD735_00845)	comGA/cglA/cilD	148890..149831 (+)	942	WP_061421835.1	competence type IV pilus ATPase ComGA	Machinery gene
FD735_RS00850 (FD735_00850)	comGB/cglB	149779..150795 (+)	1017	WP_081102569.1	competence type IV pilus assembly protein ComGB	Machinery gene
FD735_RS00855 (FD735_00855)	comGC/cglC	150797..151120 (+)	324	WP_061421834.1	competence type IV pilus major pilin ComGC	Machinery gene
FD735_RS00860 (FD735_00860)	comGD/cglD	151083..151517 (+)	435	WP_176553041.1	competence type IV pilus minor pilin ComGD	Machinery gene
FD735_RS00865 (FD735_00865)	comGE/cglE	151480..151782 (+)	303	WP_139658261.1	competence type IV pilus minor pilin ComGE	Machinery gene
FD735_RS00870 (FD735_00870)	comGF/cglF	151745..152206 (+)	462	WP_139658262.1	competence type IV pilus minor pilin ComGF	Machinery gene
FD735_RS00875 (FD735_00875)	comGG/cglG	152184..152597 (+)	414	WP_139658263.1	competence type IV pilus minor pilin ComGG	Machinery gene
FD735_RS00880 (FD735_00880)	-	152630..153220 (+)	591	WP_176553042.1	class I SAM-dependent methyltransferase	-
FD735_RS00885 (FD735_00885)	comYH	153279..154232 (+)	954	WP_139658264.1	class I SAM-dependent methyltransferase	Machinery gene
FD735_RS00890 (FD735_00890)	-	154283..155473 (+)	1191	WP_139658265.1	acetate kinase	-
FD735_RS00895 (FD735_00895)	rnpA	155623..155994 (+)	372	WP_000748120.1	ribonuclease P protein component	-
FD735_RS00900 (FD735_00900)	-	155969..156796 (+)	828	WP_176553043.1	membrane protein insertase YidC	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17828.47 Da
• Isoelectric Point: 9.5467

>NTDB_id=362617 FD735_RS00870 WP_139658262.1 151745..152206(+) (comGF/cglF) [Streptococcus sp. 1643]
MVQRSYSTLKSSKVRAFTLLESLIALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLETELERSQFEKVEAN
HLYVKQDGKDIAMGKSKSDDFRKTDSSGRGYQPMVYGLKSAQIIEENQLVRFRFQFQKGLEREFIYRVEKAKS

Nucleotide

• Length: 462 bp

>NTDB_id=362617 FD735_RS00870 WP_139658262.1 151745..152206(+) (comGF/cglF) [Streptococcus sp. 1643]
ATGGTTCAGAGAAGTTACTCGACCTTAAAGAGCAGTAAGGTAAGAGCGTTCACTCTTTTAGAATCTCTGATTGCTCTTAT
CGTCATTAGCGGAGGCTTGCTCCTCTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGTTACCAGCAGCAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAGCTAGAGACTGAGTTAGAGCGTTCGCAGTTTGAAAAGGTGGAAGCCAAT
CACCTCTATGTGAAGCAAGATGGCAAGGATATCGCTATGGGCAAGTCCAAATCAGATGATTTTCGGAAAACCGATAGCAG
CGGACGGGGCTACCAACCTATGGTTTATGGACTCAAATCAGCACAGATTATAGAAGAAAATCAATTGGTTCGCTTTCGTT
TCCAATTTCAAAAGGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGCAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

3D structure

• AlphaFold DB: A0A5B7Y3I0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus mitis SK321	86.275	100	0.863
comGF/cglF	Streptococcus mitis NCTC 12261	86.275	100	0.863
comGF/cglF	Streptococcus pneumoniae Rx1	84.967	100	0.85
comGF/cglF	Streptococcus pneumoniae D39	84.967	100	0.85
comGF/cglF	Streptococcus pneumoniae R6	84.967	100	0.85
comGF/cglF	Streptococcus pneumoniae TIGR4	84.967	100	0.85
comGF	Lactococcus lactis subsp. cremoris KW2	41.429	91.503	0.379
comYF	Streptococcus mutans UA140	41.481	88.235	0.366