Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FCN78_RS02190 Genome accession   NZ_CP040021
Coordinates   442756..444438 (-) Length   560 a.a.
NCBI ID   WP_077659062.1    Uniprot ID   A0AB36JZ45
Organism   Salinivibrio kushneri strain AL184     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 437756..449438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FCN78_RS02160 (FCN78_02160) mutT 438571..438966 (+) 396 WP_069363360.1 8-oxo-dGTP diphosphatase MutT -
  FCN78_RS02165 (FCN78_02165) yacG 439009..439209 (-) 201 WP_069363361.1 DNA gyrase inhibitor YacG -
  FCN78_RS02170 (FCN78_02170) zapD 439220..439960 (-) 741 WP_077659059.1 cell division protein ZapD -
  FCN78_RS02175 (FCN78_02175) coaE 439984..440598 (-) 615 WP_077659060.1 dephospho-CoA kinase -
  FCN78_RS02180 (FCN78_02180) pilD 440598..441467 (-) 870 WP_077659061.1 prepilin peptidase Machinery gene
  FCN78_RS02185 (FCN78_02185) pilC 441532..442746 (-) 1215 WP_069363365.1 type II secretion system F family protein Machinery gene
  FCN78_RS02190 (FCN78_02190) pilB 442756..444438 (-) 1683 WP_077659062.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FCN78_RS02195 (FCN78_02195) - 444463..444879 (-) 417 WP_077659063.1 pilin -
  FCN78_RS02200 (FCN78_02200) nadC 445126..446031 (-) 906 WP_106407178.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FCN78_RS02205 (FCN78_02205) - 446192..447187 (+) 996 WP_077659064.1 ABC transporter substrate-binding protein -
  FCN78_RS02210 (FCN78_02210) - 447184..448488 (+) 1305 WP_077456493.1 diguanylate cyclase domain-containing protein -
  FCN78_RS02215 (FCN78_02215) ampD 448513..449061 (+) 549 WP_077456495.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 61828.00 Da        Isoelectric Point: 6.2539

>NTDB_id=361673 FCN78_RS02190 WP_077659062.1 442756..444438(-) (pilB) [Salinivibrio kushneri strain AL184]
MLPTATLYQAGLLTDVQRHALGIGDGTPLSVSRLLDAGVDPTALAHTLASQHHLPFSESHQYPFKDTCYALDRRDLIQRH
QVLPLAQQGKELLLGVSDPDNTEAVADFRFSTGLNVRLVVLSQPDLDAALRHLYGADIDGTGDGITISDQDLAALTVTDS
PQEDSAELQSDQAPISRYIQQILVDAVRKNASDVHFEPFEHHYQIRFRQDGLLHTYAHPPAGVSRRLSTRLKILAQLNIA
ERRLPQDGRIKLTLTADHTVDMRVSTLPTVWGEKVVVRLLNNGQQQLTLHDLGLTEWQRQHYQDALDRPQGMVLFTGPTG
SGKTVSLYSGLRYLNQPQRNIATVEDPVEIQLEGINQVNVNPSIGLDFATALRAFLRQDPDVVMVGEIRDLDTAEIGIKA
AQTGHLVLSTLHTNSAAESITRLSHMGIAPYNLASSLTLVVAQRLVRRLCPVCKQPHPSDDPRIQYLANLYPSLTPSTLY
QANPHGCYQCYQGYQGRTGVYEVLPVTEKITSAIYRNASAVELTQLATEEGMWRLAESGVAKLNQGITSIQELTRIIQLA

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=361673 FCN78_RS02190 WP_077659062.1 442756..444438(-) (pilB) [Salinivibrio kushneri strain AL184]
ATGCTCCCAACCGCTACGTTGTATCAAGCCGGTTTATTGACCGATGTCCAGCGCCATGCGTTGGGCATTGGCGATGGCAC
ACCGCTTAGCGTCTCCCGTTTACTCGATGCCGGGGTTGACCCCACCGCGCTCGCCCACACTCTCGCGAGTCAGCACCACC
TGCCTTTTTCGGAAAGCCATCAGTACCCGTTTAAAGACACCTGTTATGCCCTAGATAGGCGCGATTTGATTCAGCGCCAT
CAAGTGTTGCCGCTGGCCCAGCAAGGCAAAGAGCTGTTGCTCGGCGTCAGTGACCCAGATAACACGGAGGCGGTCGCCGA
CTTTCGCTTTTCTACTGGGCTCAATGTCCGTTTAGTGGTACTCAGTCAGCCGGATTTGGATGCCGCGCTGCGCCACTTAT
ACGGCGCAGATATCGATGGCACCGGCGATGGCATCACCATCAGTGATCAAGACTTAGCCGCCCTGACCGTCACCGATAGC
CCACAAGAAGACAGCGCTGAGCTACAAAGTGATCAGGCGCCGATCAGTCGTTACATTCAACAGATATTGGTGGATGCGGT
ACGTAAAAACGCCTCCGACGTACACTTCGAGCCCTTTGAACATCACTACCAAATTCGCTTTCGCCAAGACGGTTTATTGC
ACACCTATGCCCACCCTCCTGCGGGGGTCTCGCGGCGGCTGTCGACTCGACTCAAAATCTTGGCCCAACTCAATATTGCC
GAACGACGTTTACCACAAGACGGGCGCATTAAGCTGACCCTCACTGCCGATCACACCGTCGATATGCGCGTCTCCACCTT
ACCGACCGTTTGGGGCGAGAAGGTGGTGGTTCGCTTGTTGAATAATGGCCAGCAGCAACTGACGCTACATGATTTAGGCT
TAACCGAATGGCAGCGCCAGCACTATCAAGACGCATTGGATAGGCCGCAAGGGATGGTTTTGTTTACCGGCCCAACGGGA
AGCGGCAAAACCGTCTCTTTGTATAGTGGGCTCAGGTATCTCAACCAGCCGCAGCGTAATATTGCCACCGTCGAAGATCC
GGTTGAGATCCAGCTAGAAGGCATCAATCAAGTCAATGTTAATCCTAGCATCGGTTTAGATTTCGCCACCGCACTCCGTG
CCTTTTTGCGCCAAGATCCCGATGTGGTGATGGTGGGAGAAATACGCGATCTCGACACCGCTGAGATCGGGATCAAGGCC
GCGCAAACCGGTCACCTGGTGTTATCAACGCTGCATACCAATTCGGCGGCCGAAAGCATCACCCGTTTATCCCATATGGG
CATTGCGCCCTACAACCTTGCCAGCTCTCTAACTTTGGTGGTGGCTCAGCGTTTGGTACGCCGTTTATGCCCTGTTTGTA
AACAGCCGCACCCATCTGATGACCCACGCATTCAGTATTTAGCCAACCTCTATCCAAGCTTAACCCCAAGCACGCTCTAC
CAAGCCAATCCCCACGGCTGTTACCAGTGCTATCAGGGTTATCAAGGACGGACTGGGGTGTACGAAGTGCTCCCCGTGAC
AGAAAAGATAACCAGCGCCATTTACCGAAATGCCAGCGCGGTGGAACTCACTCAACTGGCCACCGAAGAAGGTATGTGGC
GATTGGCCGAGTCTGGGGTTGCCAAACTCAACCAAGGGATCACCAGCATCCAAGAGCTTACCCGCATTATTCAGCTTGCC
TAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(181-448)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

53.131

99.821

0.53
  pilB Vibrio campbellii strain DS40M4

52.679

100

0.527
  pilB Vibrio cholerae strain A1552

51.693

100

0.518
  pilB Legionella pneumophila strain ERS1305867

46.538

92.857

0.432
  pilB Acinetobacter baumannii D1279779

46.08

93.393

0.43
  pilB Acinetobacter baylyi ADP1

46.108

89.464

0.413
  pilF Neisseria gonorrhoeae MS11

42.344

94.464

0.4
  pilF Thermus thermophilus HB27

39.048

93.75

0.366

Multiple sequence alignment