Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GVI48_RS07850 Genome accession   NZ_CP047697
Coordinates   1698393..1698857 (+) Length   154 a.a.
NCBI ID   WP_023090842.1    Uniprot ID   A3RJ48
Organism   Pseudomonas aeruginosa strain RD1-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1693393..1703857
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GVI48_RS07825 yacG 1693563..1693763 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  GVI48_RS07830 coaE 1693760..1694371 (-) 612 WP_003112838.1 dephospho-CoA kinase -
  GVI48_RS07835 pilD 1694368..1695240 (-) 873 WP_023090843.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  GVI48_RS07840 pilC 1695241..1696458 (-) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  GVI48_RS07845 pilB 1696462..1698162 (-) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GVI48_RS07850 pilA 1698393..1698857 (+) 465 WP_023090842.1 pilin Machinery gene
  GVI48_RS07855 - 1699015..1700328 (+) 1314 WP_023090841.1 O-antigen ligase family protein -
  GVI48_RS07865 nadC 1700509..1701357 (-) 849 WP_003122081.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16023.30 Da        Isoelectric Point: 9.0066

>NTDB_id=360858 GVI48_RS07850 WP_023090842.1 1698393..1698857(+) (pilA) [Pseudomonas aeruginosa strain RD1-3]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSSDTPGNNEYDLGFTS
STLLTGSGKGQIKIDKADTATPEISGTLGNSSGKGIAGAVITVKRDDKGVWTCGITGSPTNWKTNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=360858 GVI48_RS07850 WP_023090842.1 1698393..1698857(+) (pilA) [Pseudomonas aeruginosa strain RD1-3]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAGCGATACCCCCGGAAACAATGAATATGATCTTGGCTTTACCAGC
TCTACTCTGCTTACTGGTAGCGGTAAGGGGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
TCTGGGCAACTCTTCTGGTAAGGGTATCGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGTTCGCCGACCAACTGGAAAACCAACTACGCCCCGGCTAACTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3RJ48

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

43.949

100

0.448
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.516

100

0.448
  pilA Pseudomonas aeruginosa PAK

41.139

100

0.422
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.447

98.701

0.409
  pilA Vibrio cholerae strain A1552

41.447

98.701

0.409
  pilA Vibrio cholerae C6706

41.447

98.701

0.409
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.94

96.753

0.396
  pilA Ralstonia pseudosolanacearum GMI1000

35.882

100

0.396