Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FAZ30_RS08540 Genome accession   NZ_CP039731
Coordinates   1832496..1834190 (+) Length   564 a.a.
NCBI ID   WP_205676668.1    Uniprot ID   -
Organism   Aquitalea aquatilis strain THG-DN7.12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1827496..1839190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FAZ30_RS08520 ccsA 1828160..1828981 (+) 822 WP_124645431.1 cytochrome c biogenesis protein CcsA -
  FAZ30_RS08525 comE 1829045..1829398 (+) 354 WP_124645432.1 ComEA family DNA-binding protein Machinery gene
  FAZ30_RS08530 - 1829447..1830847 (-) 1401 WP_124645433.1 deoxyribodipyrimidine photo-lyase -
  FAZ30_RS08535 - 1831189..1832262 (+) 1074 WP_124645434.1 porin -
  FAZ30_RS08540 pilF 1832496..1834190 (+) 1695 WP_205676668.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FAZ30_RS08545 pilC 1834208..1835428 (+) 1221 WP_124645436.1 type II secretion system F family protein Machinery gene
  FAZ30_RS08550 pilD 1835431..1836300 (+) 870 WP_124645437.1 A24 family peptidase Machinery gene
  FAZ30_RS08555 coaE 1836290..1836901 (+) 612 WP_124645438.1 dephospho-CoA kinase -
  FAZ30_RS08560 zapD 1836941..1837699 (+) 759 WP_059284846.1 cell division protein ZapD -
  FAZ30_RS08565 yacG 1837703..1837900 (+) 198 WP_103524355.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 61424.82 Da        Isoelectric Point: 5.5630

>NTDB_id=360543 FAZ30_RS08540 WP_205676668.1 1832496..1834190(+) (pilF) [Aquitalea aquatilis strain THG-DN7.12]
MSGLGRALVQNNALLLQDAEAIQRLAQASGISFVEQLIASKKMSAVEVATFASGVFGYPLLDMGAIDPALLPKGMVDEEL
IGSRRLVPLFKRGNRLYIGTSDPTQTSAFHAITFKTGLTVEVVVVEDDKLSKVIGKYTEDATAAIKALENEDFGDLEFVD
PDAPTAHSETNQPDVDDAPVVKFIHKVLLDAITGGASDVHFEPYEKFYRIRYRVDGQLKEIAQPPLLLKEKIASRIKVIS
RLDISEKRVPQDGRLKLVLSKTHSIDFRVSTLPTLYGEKIVMRILDASAASLDIEQLGFEPEQKKLLLEAIGRPYGMVLV
TGPTGSGKTVSLYTCLNLLNKPDINISTAEDPVEINLPGINQVNVNEKAGLTFSSALKAFLRQDPDVIMVGEIRDFETAD
ISIKAAQTGHMVFSTLHTNNAPATLTRLLNMGVAPFNVASSVLLIMAQRLARRLCGHCKAPVDIPDTALLRAGFRQEELD
GSWKPYGPVGCDECRGSGYKGRTGIYEVMPITDAMTRLIMQNGTAIDINDLALKEGMVDLRHAGLLKVKRGLTSLAEIEA
VTND

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=360543 FAZ30_RS08540 WP_205676668.1 1832496..1834190(+) (pilF) [Aquitalea aquatilis strain THG-DN7.12]
ATATCTGGCCTGGGGCGGGCACTTGTTCAGAATAATGCCTTGCTGTTGCAGGATGCAGAGGCGATTCAACGTCTGGCGCA
GGCTTCCGGTATCAGCTTTGTCGAGCAATTGATCGCCAGCAAGAAGATGTCGGCCGTGGAGGTGGCGACCTTTGCCTCTG
GCGTGTTTGGCTACCCTTTGCTGGATATGGGCGCGATTGATCCTGCCCTGTTACCCAAGGGTATGGTTGATGAAGAATTG
ATCGGCAGTCGCCGTCTGGTTCCCTTGTTCAAGCGTGGCAATAGACTTTATATCGGCACTTCCGACCCTACCCAGACCTC
GGCCTTTCATGCCATTACCTTCAAGACCGGCCTGACGGTTGAAGTGGTGGTGGTTGAGGATGACAAGCTGTCCAAGGTGA
TCGGCAAGTACACCGAGGATGCTACTGCTGCCATCAAGGCCCTTGAAAACGAGGATTTTGGTGATCTGGAGTTTGTCGAT
CCGGATGCACCTACCGCACACAGCGAAACCAATCAGCCAGATGTCGATGATGCGCCGGTGGTCAAGTTCATCCATAAGGT
GTTGCTGGATGCGATTACGGGTGGTGCTTCCGACGTTCACTTTGAACCGTATGAAAAATTTTATCGTATTCGTTACCGGG
TCGATGGCCAACTCAAGGAAATTGCCCAGCCGCCCTTGCTGCTGAAAGAAAAAATCGCCTCTCGCATCAAGGTGATTTCC
CGCCTGGATATTTCAGAAAAGCGAGTTCCTCAGGATGGCCGCTTGAAGCTGGTTCTTTCCAAGACCCACTCGATTGATTT
TCGCGTGAGTACCTTGCCGACCTTGTATGGCGAGAAGATCGTGATGCGTATTCTTGATGCCTCCGCGGCATCGCTGGATA
TCGAGCAGCTGGGGTTCGAGCCGGAACAGAAAAAATTGCTGCTAGAGGCAATTGGTCGCCCGTATGGCATGGTGCTGGTT
ACCGGGCCAACCGGTAGTGGTAAAACAGTGTCGCTTTACACCTGCCTGAATCTCCTGAATAAGCCCGATATCAATATTTC
CACGGCAGAAGACCCGGTCGAAATCAACTTGCCGGGCATCAATCAGGTCAACGTCAACGAAAAGGCAGGTCTGACTTTTT
CTTCGGCCTTGAAAGCCTTTTTGCGGCAAGATCCCGATGTGATCATGGTGGGTGAAATCCGTGACTTCGAGACGGCGGAT
ATTTCCATCAAGGCCGCGCAGACTGGTCACATGGTATTCTCCACGCTGCATACCAATAATGCTCCGGCCACATTGACGCG
TTTGCTCAACATGGGGGTGGCGCCATTTAACGTGGCCAGCTCCGTACTGCTTATCATGGCGCAGCGTCTGGCGCGTCGTT
TGTGCGGCCATTGCAAGGCCCCAGTGGATATACCTGATACGGCCTTGCTGAGGGCTGGGTTCCGGCAGGAAGAATTGGAT
GGCAGCTGGAAACCCTATGGCCCGGTCGGTTGCGATGAGTGCCGCGGTTCCGGCTATAAGGGTCGTACCGGTATTTATGA
GGTGATGCCGATTACCGATGCGATGACCCGACTAATCATGCAAAATGGCACGGCGATTGATATCAATGATCTGGCCTTGA
AAGAGGGCATGGTCGATCTGCGGCATGCAGGCCTGCTCAAGGTCAAGCGAGGGCTGACCTCGCTGGCTGAAATTGAGGCA
GTGACCAACGACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)

Type II/IV secretion system protein (T2SSE)

(186-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

56.966

100

0.573
  pilB Acinetobacter baumannii D1279779

54.371

100

0.551
  pilB Acinetobacter baylyi ADP1

54.867

100

0.55
  pilB Legionella pneumophila strain ERS1305867

53.147

100

0.539
  pilB Vibrio cholerae strain A1552

50.743

95.39

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

47.518

100

0.475
  pilB Vibrio campbellii strain DS40M4

46.018

100

0.461
  pilF Thermus thermophilus HB27

37.937

100

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

93.794

0.371

Multiple sequence alignment