Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E2P79_RS16440 Genome accession   NZ_CP039611
Coordinates   3479807..3481513 (+) Length   568 a.a.
NCBI ID   WP_136614167.1    Uniprot ID   -
Organism   Aeromonas schubertii strain LF1708     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3474807..3486513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2P79_RS16415 (E2P79_16415) pdhR 3476143..3476898 (-) 756 WP_050666604.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  E2P79_RS16420 (E2P79_16420) ampD 3477164..3477736 (-) 573 WP_136614163.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  E2P79_RS16425 (E2P79_16425) - 3477761..3478243 (+) 483 WP_136614164.1 TIGR02281 family clan AA aspartic protease -
  E2P79_RS16430 (E2P79_16430) nadC 3478249..3479109 (+) 861 WP_136614165.1 carboxylating nicotinate-nucleotide diphosphorylase -
  E2P79_RS16435 (E2P79_16435) - 3479370..3479807 (+) 438 WP_136614166.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  E2P79_RS16440 (E2P79_16440) pilB 3479807..3481513 (+) 1707 WP_136614167.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  E2P79_RS16445 (E2P79_16445) pilC 3481534..3482772 (+) 1239 WP_050666599.1 type II secretion system F family protein Machinery gene
  E2P79_RS16450 (E2P79_16450) pilD 3482798..3483667 (+) 870 WP_136614168.1 A24 family peptidase Machinery gene
  E2P79_RS16455 (E2P79_16455) coaE 3483678..3484292 (+) 615 WP_136614169.1 dephospho-CoA kinase -
  E2P79_RS16460 (E2P79_16460) zapD 3484331..3485053 (+) 723 WP_050666596.1 cell division protein ZapD -
  E2P79_RS16465 (E2P79_16465) yacG 3485063..3485251 (+) 189 WP_050666595.1 DNA gyrase inhibitor YacG -
  E2P79_RS16470 (E2P79_16470) mutT 3485283..3485693 (-) 411 WP_050666594.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62590.84 Da        Isoelectric Point: 5.6627

>NTDB_id=360088 E2P79_RS16440 WP_136614167.1 3479807..3481513(+) (pilB) [Aeromonas schubertii strain LF1708]
MTSSPHSGLAISLAASSLISEQDSQRFVSQAKAQRKPFVTFLIENEILGSKELADFCEQEYGVPLLDLDAFDVAEIPQKY
INQKLIEKHHALPIYTQGHTLYIAMSDPTNVSALEEFGFNFGLHTEALLVEEEKLVHAIGKLLESDSDSLGMESIDEAEI
SDLEVADEQSRLDETVSTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKAMDMRVNTLPTMWGEKVVIRLLDSSAARLNIEQLGFDDKQKALYLDALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTIERNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRAFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCPHCKTPEAIPEAELLKLGYS
QQQVEAGIQLYHAVGCKECSGGYKGRVGLYEMMSMSEHIARIIMQGGNSLLISAQAQKEGMRTLRQSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=360088 E2P79_RS16440 WP_136614167.1 3479807..3481513(+) (pilB) [Aeromonas schubertii strain LF1708]
ATGACCTCTAGCCCCCATAGCGGCCTGGCTATTAGCCTGGCCGCTTCCTCCCTTATCAGTGAGCAGGACTCACAACGCTT
CGTGAGTCAGGCCAAGGCCCAGCGCAAACCGTTTGTCACCTTCCTCATTGAAAACGAGATTCTCGGCAGCAAGGAACTCG
CCGATTTTTGTGAGCAGGAGTATGGTGTACCTCTGCTGGATCTGGATGCCTTCGATGTAGCCGAGATCCCGCAGAAATAC
ATCAACCAGAAGCTGATCGAAAAGCATCACGCCCTCCCCATCTACACTCAGGGCCATACCCTCTATATCGCCATGTCAGA
CCCGACCAATGTGTCGGCACTGGAGGAGTTTGGCTTTAACTTCGGACTCCACACCGAGGCGTTGCTGGTCGAAGAGGAGA
AGCTGGTCCACGCCATCGGCAAGCTGCTGGAGAGCGACAGCGATTCCCTCGGCATGGAGAGCATCGACGAGGCGGAGATC
TCGGATCTCGAGGTGGCCGACGAGCAGAGTCGGCTCGATGAGACGGTCAGCACCGCCGACGATGATGCCCCCATCGTCAA
ATACATCAACAAGATCATGATGGACGCCATCAAGCGGGGAGCATCCGACCTGCACTTCGAGCCCTACGAGACCAAGTACC
GCATCCGTTTTCGTATCGACGGCATACTCCATGAGGTGGCCACACCGCCGGTCAACCTGGCCAACCGCTTCTCGGCCCGT
CTCAAGGTGATGGCCCGCCTCGACATCGCCGAGCGCCGCCTGCCCCAGGATGGACGCATCAAGCTCAAGCTGTCGCGCAA
CAAGGCGATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAGAAGGTAGTTATCCGCCTGCTCGACTCCTCGG
CGGCGCGCCTCAACATAGAGCAGCTCGGCTTTGACGACAAACAGAAAGCCCTCTATCTGGATGCCCTCTCCAAGCCTCAG
GGGATGATACTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGTCTCCCTCTATACGGGCCTCAACATTCTCAACACCAT
AGAGCGCAATATCTCTACCGCCGAAGACCCGGTCGAGATTAACTTGCCCGGCGTCAATCAGGTACAGGTCAACCCCAAGG
CGGGGCTGACCTTTGCCAGCGCCCTGCGCGCCTTCCTGCGCCAAGACCCGGATGTGGTGATGGTCGGCGAGATCCGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCGGCCCAGACCGGCCACCTGGTTCTCTCCACCCTGCACACCAACTCCGCCGC
CGAGACCCTCACCCGGATGATGAACATGGGAGTGCCGGCGTTTAACATCGCCTCCTCGGTGACCCTCATCATGGCCCAAC
GGCTGGCACGCAAACTCTGCCCTCACTGCAAGACGCCCGAGGCGATCCCCGAGGCGGAGCTGCTCAAACTCGGCTACAGC
CAACAACAGGTCGAGGCGGGGATCCAACTCTACCATGCGGTGGGGTGCAAGGAGTGTTCGGGGGGTTATAAGGGGCGTGT
CGGCCTCTATGAGATGATGTCCATGTCGGAACATATCGCCCGCATCATCATGCAGGGAGGCAACTCCTTGCTGATTTCCG
CCCAGGCCCAGAAGGAGGGGATGCGCACCCTGCGTCAATCCGGGCTTGAGAAGGCGCGCCTTGGGGTCACCAGCCTGGCC
GAGGTGAATCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.674

99.824

0.546
  pilB Acinetobacter baylyi ADP1

53.86

100

0.54
  pilB Legionella pneumophila strain ERS1305867

56.747

95.246

0.54
  pilB Vibrio cholerae strain A1552

54.078

99.296

0.537
  pilB Vibrio parahaemolyticus RIMD 2210633

51.418

99.296

0.511
  pilB Vibrio campbellii strain DS40M4

50.442

99.472

0.502
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.616

96.655

0.373
  pilF Thermus thermophilus HB27

39.405

94.718

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.355

85.211

0.361

Multiple sequence alignment