Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GUJ28_RS14765 Genome accession   NZ_CP047576
Coordinates   3113110..3114606 (+) Length   498 a.a.
NCBI ID   WP_001040668.1    Uniprot ID   -
Organism   Escherichia coli strain 94EC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3108110..3119606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GUJ28_RS14735 (GUJ28_14735) tusA 3108357..3108602 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  GUJ28_RS14740 (GUJ28_14740) zntA 3108704..3110902 (-) 2199 WP_000106527.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  GUJ28_RS14745 (GUJ28_14745) yhhN 3110976..3111602 (-) 627 WP_000964718.1 lysoplasmalogenase -
  GUJ28_RS14750 (GUJ28_14750) yhhM 3111743..3112102 (+) 360 WP_000042901.1 DUF2500 domain-containing protein -
  GUJ28_RS14755 (GUJ28_14755) yhhL 3112105..3112374 (-) 270 WP_001295207.1 DUF1145 family protein -
  GUJ28_RS14760 (GUJ28_14760) rsmD 3112364..3112960 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  GUJ28_RS14765 (GUJ28_14765) pilA 3113110..3114606 (+) 1497 WP_001040668.1 signal recognition particle-docking protein FtsY Machinery gene
  GUJ28_RS14770 (GUJ28_14770) ftsE 3114609..3115277 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  GUJ28_RS14775 (GUJ28_14775) ftsX 3115270..3116328 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  GUJ28_RS14780 (GUJ28_14780) rpoH 3116573..3117427 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  GUJ28_RS14785 (GUJ28_14785) livJ 3117699..3118802 (+) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  GUJ28_RS14790 (GUJ28_14790) panM 3119001..3119384 (-) 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54655.50 Da        Isoelectric Point: 4.1560

>NTDB_id=360090 GUJ28_RS14765 WP_001040668.1 3113110..3114606(+) (pilA) [Escherichia coli strain 94EC]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPVPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNVDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=360090 GUJ28_RS14765 WP_001040668.1 3113110..3114606(+) (pilA) [Escherichia coli strain 94EC]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCAGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAGGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGTGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCAGAAGAAGAAGCGGCTAAGGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTAGTTCCTCCGGCAGAAGAAGAGCAGCCGGTTGAAGAAATTGCCCAGGA
GCAAGAAAAACCGACCAAAGAAGGCTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAACAGCTTTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATAATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGTGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCTGCGAAGG
CGCGTAATGTTGACGTACTGATTGCCGATACCGCCGGACGCTTGCAGAACAAATCGCACCTGATGGAAGAGCTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.571

77.309

0.375

Multiple sequence alignment