Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   E8E00_RS12405 Genome accession   NZ_CP039516
Coordinates   2659907..2661652 (-) Length   581 a.a.
NCBI ID   WP_096631586.1    Uniprot ID   -
Organism   Salinivibrio sp. YCSC6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2654907..2666652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8E00_RS12380 (E8E00_12380) - 2655469..2655657 (+) 189 WP_069589884.1 DUF2970 domain-containing protein -
  E8E00_RS12385 (E8E00_12385) dam 2655671..2656492 (-) 822 WP_069589882.1 adenine-specific DNA-methyltransferase -
  E8E00_RS12390 (E8E00_12390) - 2656565..2658130 (-) 1566 WP_096630633.1 AAA family ATPase -
  E8E00_RS12395 (E8E00_12395) aroB 2658142..2659221 (-) 1080 WP_096630631.1 3-dehydroquinate synthase -
  E8E00_RS12400 (E8E00_12400) aroK 2659243..2659761 (-) 519 WP_069589875.1 shikimate kinase AroK -
  E8E00_RS12405 (E8E00_12405) pilQ 2659907..2661652 (-) 1746 WP_096631586.1 type IV pilus secretin PilQ Machinery gene
  E8E00_RS12410 (E8E00_12410) - 2661669..2662181 (-) 513 WP_069589873.1 pilus assembly protein PilP -
  E8E00_RS12415 (E8E00_12415) - 2662178..2662789 (-) 612 WP_069589871.1 type 4a pilus biogenesis protein PilO -
  E8E00_RS12420 (E8E00_12420) - 2662786..2663364 (-) 579 WP_096630628.1 PilN domain-containing protein -
  E8E00_RS12425 (E8E00_12425) pilM 2663355..2664269 (-) 915 WP_096630626.1 pilus assembly protein PilM -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63289.87 Da        Isoelectric Point: 6.5390

>NTDB_id=359450 E8E00_RS12405 WP_096631586.1 2659907..2661652(-) (pilQ) [Salinivibrio sp. YCSC6]
MIRPYLSRTFSQSVRPVWLILLVLMFSLGSVQVAAKSMLTDIAVKRLDNGDAQVSLTFSDAAVTADFRQPIPQQVTLRLP
NVQLKQALLAVLDTRSRQTPVTEIETFQAPESARIVLRAEQAVEFDYQQQGKVLTIRVSAAKKAPSATALAQKGAAISIN
FQDIPVRTVLQLIAEQNDFNLVTSDSVQGNITMRIDGVPWQDVLDVILRVKGLDKRQRGQVMLVAPSAELMAQEKQALEM
RQQADALLPLTSEVLTVNYAKASDIAALLKGQGEGVSLLSPRGAVSLDERTNALIIKDNQKNLARIRTLVRHLDVPVKQV
EIEARIVTVEEGSVDELGVRWGILNRNGSTSIGSSIESNLMWDRNMYNSDDEALTGSGNDSLPVDNFLNVNLAASNPAAS
SLALQVAKLGSDLLLDLELSALESENKAEIVSSPRLITTNKKAAYIEQGTELPYLEASSSGAVAVSFKKAVLSLTVTPQI
TPENELVLDLKVTQDMPEKSVVAGKGEAMSISTKRINTQVLARNGETVVLGGIFQHTMINKETKVPLLGDIPLFGNLFKH
RLKDHAKRELLIFVTPKIVTG

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=359450 E8E00_RS12405 WP_096631586.1 2659907..2661652(-) (pilQ) [Salinivibrio sp. YCSC6]
ATGATACGCCCTTACCTTTCCCGCACGTTCTCGCAAAGCGTTCGCCCAGTCTGGCTCATACTGCTGGTACTGATGTTCAG
CCTGGGTTCAGTGCAGGTTGCCGCAAAGAGTATGCTGACTGATATCGCAGTGAAGCGTCTAGATAATGGTGATGCTCAGG
TCTCGCTGACGTTTTCTGATGCCGCGGTAACGGCTGACTTTCGTCAACCCATCCCTCAGCAAGTCACGCTACGCTTGCCG
AATGTACAGCTAAAACAGGCATTACTTGCGGTTCTCGATACTCGCAGCCGTCAGACGCCAGTGACTGAGATTGAGACCTT
TCAGGCCCCTGAGAGTGCTCGCATTGTACTGCGTGCTGAGCAGGCCGTGGAGTTTGATTATCAGCAGCAAGGAAAGGTGT
TGACCATCCGCGTCTCTGCGGCAAAAAAAGCGCCTTCTGCGACGGCCTTAGCGCAAAAAGGGGCGGCGATCTCGATTAAC
TTCCAAGACATTCCGGTCCGTACCGTGTTGCAGCTGATTGCTGAACAGAATGATTTTAACCTTGTGACCTCGGACTCGGT
ACAAGGCAATATCACCATGCGCATTGATGGTGTGCCTTGGCAAGATGTATTGGATGTGATTTTGCGTGTGAAGGGACTGG
ATAAGCGCCAACGGGGACAAGTGATGCTCGTTGCTCCGAGCGCTGAGCTGATGGCACAAGAAAAGCAAGCACTCGAAATG
CGTCAGCAGGCCGATGCCTTACTGCCTCTGACCTCAGAAGTACTCACGGTGAACTATGCCAAAGCCAGTGATATCGCCGC
CCTGCTCAAAGGTCAAGGCGAAGGGGTCAGTTTACTGTCTCCTCGCGGGGCGGTGTCGCTGGATGAGCGCACCAATGCGT
TGATCATTAAAGATAATCAAAAGAACTTAGCCCGTATTCGCACCTTGGTCCGCCACTTGGATGTGCCGGTGAAGCAAGTT
GAAATTGAAGCGCGGATTGTCACGGTTGAAGAAGGGTCTGTCGATGAGCTGGGAGTGCGCTGGGGTATTTTAAATCGCAA
CGGTTCAACCAGTATCGGCTCTTCGATAGAAAGCAACCTGATGTGGGACCGTAACATGTACAATTCCGATGACGAGGCAT
TGACCGGATCGGGCAATGATTCTCTGCCGGTGGACAATTTTTTGAATGTGAACTTGGCCGCCTCCAACCCTGCCGCGTCA
AGTCTGGCACTGCAAGTGGCTAAATTGGGCAGTGATTTATTGCTGGATCTCGAGTTATCGGCGCTGGAAAGTGAAAACAA
AGCAGAAATTGTATCGAGCCCGCGCTTAATCACCACCAATAAAAAAGCGGCATATATCGAGCAGGGGACCGAATTACCTT
ATCTTGAAGCCTCTTCCAGCGGTGCTGTGGCGGTGTCGTTTAAAAAAGCAGTACTTAGCCTTACCGTGACGCCCCAAATT
ACCCCAGAGAATGAGTTAGTGCTGGATTTGAAAGTCACCCAAGATATGCCAGAGAAGTCGGTGGTGGCAGGTAAAGGGGA
GGCCATGTCGATCAGTACCAAGCGCATTAATACGCAGGTATTGGCGAGGAATGGTGAAACGGTGGTGCTGGGAGGGATCT
TCCAACACACCATGATTAACAAAGAAACCAAGGTGCCATTACTCGGGGATATTCCGCTGTTTGGAAATCTATTCAAACAC
CGTCTGAAAGATCATGCAAAACGCGAGCTTCTGATCTTTGTGACCCCTAAAATCGTCACAGGGTAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(421-579)

Bacterial type II/III secretion system short domain (Secretin_N)

(253-319)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

55.263

91.566

0.506
  pilQ Vibrio cholerae strain A1552

57.004

88.468

0.504
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

57.004

88.468

0.504
  comQ Acinetobacter baylyi ADP1

38.648

99.312

0.384
  pilQ Acinetobacter baumannii strain A118

37.119

100

0.377
  pilQ Acinetobacter baumannii D1279779

37.119

100

0.377
  pilQ Legionella pneumophila strain ERS1305867

38.959

95.869

0.373
  pilQ Legionella pneumophila strain Lp02

38.959

95.869

0.373
  pilQ Pseudomonas aeruginosa PAK

38.339

97.418

0.373

Multiple sequence alignment