Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   E6B08_RS28440 Genome accession   NZ_CP039371
Coordinates   6154983..6156257 (-) Length   424 a.a.
NCBI ID   WP_136917014.1    Uniprot ID   A0A4D6XQH5
Organism   Pseudomonas putida strain 1290     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6149983..6161257
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E6B08_RS28425 (E6B08_28425) - 6151646..6153256 (-) 1611 WP_136917012.1 AAA family ATPase -
  E6B08_RS28430 (E6B08_28430) aroB 6153272..6154369 (-) 1098 WP_136917013.1 3-dehydroquinate synthase -
  E6B08_RS28435 (E6B08_28435) aroK 6154460..6154978 (-) 519 WP_133325012.1 shikimate kinase AroK -
  E6B08_RS28440 (E6B08_28440) pilQ 6154983..6156257 (-) 1275 WP_136917014.1 type IV pilus secretin PilQ Machinery gene
  E6B08_RS28445 (E6B08_28445) - 6156175..6157134 (-) 960 WP_192938599.1 pilus assembly protein PilP -
  E6B08_RS28450 (E6B08_28450) - 6157185..6157721 (-) 537 WP_136917016.1 PilN domain-containing protein -
  E6B08_RS28455 (E6B08_28455) pilM 6157721..6158569 (-) 849 WP_136917017.1 type IV pilus biogenesis protein PilM -
  E6B08_RS28460 (E6B08_28460) - 6158750..6161200 (+) 2451 WP_416194417.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 424 a.a.        Molecular weight: 45869.86 Da        Isoelectric Point: 6.1932

>NTDB_id=358231 E6B08_RS28440 WP_136917014.1 6154983..6156257(-) (pilQ) [Pseudomonas putida strain 1290]
MKLHTRWKWLLALVLPIPLASATAHRGETLSLNVQDVQVRAVLQLLADHAGVNLVASDAVQGSVTLRLQDVPWEQALDLI
LRSKGLARREEGNVLLVAPVGELVGPAGEPYAGEPHALPLQPLRRELLPVHHAKAGELAELLLATLADDGILTGRGSLSV
DPRTNTLVAYQPAGRLAELRKLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGRELHGDTAQLGKDLFVDLGVERAGA
GVGLGLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSVSFREASLSLEVTPQITP
DGKVIMAVRVTKDEPDYVNALNDVPPIRKNEVNAKVRVADGETIVIGGVYSTTQNKVVDKVPFLGDLPYVGRLFRRDVLQ
EKKSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1275 bp        

>NTDB_id=358231 E6B08_RS28440 WP_136917014.1 6154983..6156257(-) (pilQ) [Pseudomonas putida strain 1290]
ATGAAGTTGCACACAAGGTGGAAATGGCTGTTGGCGCTGGTATTGCCGATCCCGCTGGCATCGGCGACGGCCCATCGCGG
TGAGACGCTGTCGCTGAACGTTCAGGATGTGCAGGTACGCGCCGTACTCCAGCTATTGGCGGATCATGCCGGGGTCAACC
TGGTGGCCAGCGATGCGGTCCAGGGCAGTGTCACCCTGCGCCTTCAGGACGTGCCTTGGGAGCAGGCCCTGGACCTGATC
CTGCGTAGCAAGGGCCTGGCCAGGCGCGAGGAGGGCAATGTGCTGTTGGTCGCGCCCGTCGGTGAACTCGTCGGGCCAGC
GGGCGAGCCCTATGCCGGGGAGCCTCATGCGCTGCCCTTGCAACCCCTGCGCCGCGAGTTGCTACCGGTGCACCATGCCA
AGGCAGGCGAATTGGCAGAGCTGCTGCTGGCCACCCTCGCCGACGATGGCATCCTCACCGGCCGTGGCAGCCTGAGCGTC
GATCCACGCACCAACACCTTGGTGGCGTACCAGCCGGCCGGGCGCCTGGCCGAATTGCGCAAACTGGTCGCGCAGCTGGA
CGTGCCGGTGCGCCAGGTGGCGATCGAGGCGCGGATCGTCGAGGCCAATGTCGACTACGAGAAGAGTCTGGGCGTGCGTT
GGGGCAGGGAGCTGCATGGCGATACCGCGCAACTGGGCAAGGACCTGTTCGTCGACCTGGGTGTCGAGCGGGCAGGCGCA
GGGGTGGGGTTGGGCCTGTTGCGCGGCGATGTGTTGCTGGACCTCGAACTGAGTGCCATGGAAAAAAGCGGCAATGGCGA
GATCATCTCGCAACCCAAGGTGGTGACCGCCGACAAGGAAACCGCGCGGATCCTCAAGGGCACCGAGGTTCCGTACCAGG
AAACCAGCAAGAGCGGCGCCACCTCGGTGTCGTTCCGCGAGGCCTCGTTGTCGCTGGAGGTCACTCCGCAGATCACGCCC
GATGGCAAGGTCATCATGGCCGTGCGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACGACGTACCGCCGAT
CCGCAAGAACGAAGTCAATGCCAAGGTGCGCGTGGCCGATGGCGAGACCATCGTGATCGGCGGGGTGTACTCGACCACGC
AAAACAAAGTGGTCGACAAGGTGCCATTTCTGGGCGATCTGCCGTATGTTGGGCGGCTATTTCGACGCGATGTATTACAA
GAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Secretin and TonB N terminus short domain (STN)

(53-99)

Bacterial type II and III secretion system protein (Secretin)

(259-415)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4D6XQH5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.81

100

0.606
  pilQ Acinetobacter baumannii D1279779

41.703

100

0.45
  pilQ Acinetobacter baumannii strain A118

41.703

100

0.45
  comQ Acinetobacter baylyi ADP1

42.697

100

0.448
  pilQ Legionella pneumophila strain Lp02

42.308

98.113

0.415
  pilQ Legionella pneumophila strain ERS1305867

42.308

98.113

0.415
  pilQ Vibrio campbellii strain DS40M4

39.352

100

0.401
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.667

99.292

0.394
  pilQ Vibrio cholerae strain A1552

39.667

99.292

0.394

Multiple sequence alignment