Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   E4A35_RS22045 Genome accession   NZ_CP039269
Coordinates   4230890..4233229 (-) Length   779 a.a.
NCBI ID   WP_000941260.1    Uniprot ID   A0A4V3X114
Organism   Bacillus cereus strain MH19     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4225890..4238229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4A35_RS27595 - 4227116..4227292 (-) 177 WP_001242605.1 hypothetical protein -
  E4A35_RS22030 - 4227478..4227918 (-) 441 WP_001266954.1 D-tyrosyl-tRNA(Tyr) deacylase -
  E4A35_RS22035 relA 4227936..4230119 (-) 2184 WP_001262809.1 GTP diphosphokinase -
  E4A35_RS22040 - 4230330..4230842 (-) 513 WP_000346215.1 adenine phosphoribosyltransferase -
  E4A35_RS22045 recJ 4230890..4233229 (-) 2340 WP_000941260.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  E4A35_RS22050 - 4233331..4234224 (-) 894 WP_000409725.1 cation diffusion facilitator family transporter -
  E4A35_RS22055 secDF 4234378..4236642 (-) 2265 WP_001119059.1 protein translocase subunit SecDF -
  E4A35_RS22060 - 4236913..4237197 (-) 285 WP_000886975.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88135.73 Da        Isoelectric Point: 4.9330

>NTDB_id=357784 E4A35_RS22045 WP_000941260.1 4230890..4233229(-) (recJ) [Bacillus cereus strain MH19]
MLQPKTRWKEKEYNGERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGADVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDATPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEDNKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLCIDPVKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQAEVILTEEDFIPITAVDAFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANLAELPKEKITMVYFSKEVLHKFSLEDYKEHMMHASEVTELDEQYIVLLDLPKGTDELRD
LFKVGFPSRIYTLFYQENNHLFSTVPTRDHFKWYYSFLSQKSPFSLRQYGEQLCQHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=357784 E4A35_RS22045 WP_000941260.1 4230890..4233229(-) (recJ) [Bacillus cereus strain MH19]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGGCGAGCGAGTGAGTGAATTAGCAAGTAAATTACAATT
ATCACCACTTGTCGTTTCGTTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGACTACGATGCGGACGGAGTAAGTAGTACGACTGTATTATATCTTGCCCTACAAGAATT
AGGTGCAGACGTAGAATTTTATATCCCAAACCGTTTTACAGAAGGTTATGGACCAAATGAAGAAGCATTCCGTTGGGCGC
ATAGCGCAGGATTCTCACTCATTATTACTGTTGATACTGGTATCGCAGCTGTACATGAAGCGAAAGTAGCGAAGGAACTT
GGGATAGATCTTATTATTACGGATCACCATGAGCCGCCACCAGAATTGCCAGAAGCACTTGCGATTATTCATCCGAAATT
AGATGGCGGTGTGTATCCGTTTCACTATTTAGCGGGCGTTGGTGTTGCGTTTAAAGTTGCGCATGCACTACTGGGCCGTG
TACCAGAACATTTATTAGAAATTGCAGTAATTGGTACAGTAGCCGATTTAGTATCGCTTCACGGTGAAAATAGATTGCTA
GTGAAGCGCGGTTTAAAACATATGCGCATGACGAAAAATATCGGACTAAAGGCGCTATTTAAAGTAGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCAATCGCACCTCGTATTAATGCAGTTGGACGTTTAGAAGATGCTACGC
CGGCTGTACATCTTTTATTATCAGAGGATCCGGAAGAAGCGAAAGAGCTGGCTGAAGAAATTGATGAACTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAAGCTATCGCTGAGGTGGAAAATAATTTCCCGCCTGAAGACAATAAAGT
ATTAGTGCTTGCAAAAGAAGGCTGGAATCCAGGTGTAATTGGAATTGTAGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CAACGATTGTATTATGCATTGATCCTGTAAAAGAAACTGCAAAAGGTTCAGCGCGTAGTATTGCAGGATTCGATTTGTTT
GCAAACTTGTCAGATTGTAGAGAGTTATTGCCACACTTCGGGGGACATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGCCGCCGTTTAAATGAGCAAGCAGAGGTAATTTTAACGGAAGAAGATTTTATCCCAATTACAG
CTGTCGATGCTTTTTGTAAAGTGGAGGACGTAACACTTGCAGCAATCGAGGATATGCAAAAGTTGGCACCATTTGGTGTT
GGAAATCCAAAGCCACGTATTGCGGTGAAGGATGCAGAGTTAGAAAGTATTCGTGCAATCGGATCAGACGGTTCTCATTT
AAAAATGGCTCTTCGTGATGGACAGGCAACACTTGATACAATTGGGTTTGGATTTGGAGCGTATGCGAAAGAAATTTCTC
CAGTTGCAAAAGTATCTGTAATCGGGGAAGCGTCTATTAATGAATGGAATAATTTTAAGAAGCCGCAATTAATGGTGCAA
GATATTGCTGTAGAGGCATGGCAATTATTTGATTGGCGCAGTATGCGTAACGTAGAAGCAAATTTAGCAGAACTTCCGAA
AGAAAAAATAACGATGGTGTATTTTTCTAAAGAGGTATTGCATAAATTTTCTTTAGAAGACTATAAAGAGCATATGATGC
ATGCATCAGAAGTAACCGAGTTAGATGAGCAATACATCGTGTTACTTGATTTACCAAAAGGAACAGATGAATTACGAGAT
CTATTTAAAGTTGGGTTCCCGTCGCGAATTTATACGCTATTTTATCAAGAGAATAATCATTTATTTAGTACTGTTCCAAC
GAGGGATCACTTTAAATGGTACTATTCATTCTTGAGCCAAAAATCACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCAACATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAGTTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGCTAGGGTAA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4V3X114

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.842

99.358

0.525

Multiple sequence alignment