Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   E5678_RS12035 Genome accession   NZ_CP039252
Coordinates   2677111..2678328 (+) Length   405 a.a.
NCBI ID   WP_136178750.1    Uniprot ID   A0A4P7RCP2
Organism   Hydrogenophaga sp. PAMC20947     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2672111..2683328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5678_RS12015 (E5678_12015) rpmA 2673263..2673520 (-) 258 WP_136178748.1 50S ribosomal protein L27 -
  E5678_RS12020 (E5678_12020) rplU 2673536..2673847 (-) 312 WP_066092426.1 50S ribosomal protein L21 -
  E5678_RS12025 (E5678_12025) - 2674122..2675051 (+) 930 WP_136180749.1 polyprenyl synthetase family protein -
  E5678_RS12030 (E5678_12030) pilB 2675340..2677079 (+) 1740 WP_136178749.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E5678_RS12035 (E5678_12035) pilC 2677111..2678328 (+) 1218 WP_136178750.1 type II secretion system F family protein Machinery gene
  E5678_RS12040 (E5678_12040) - 2678328..2679185 (+) 858 WP_136178751.1 A24 family peptidase -
  E5678_RS12045 (E5678_12045) coaE 2679257..2679790 (+) 534 WP_247597027.1 dephospho-CoA kinase -
  E5678_RS12050 (E5678_12050) zapD 2679829..2680584 (+) 756 WP_136178753.1 cell division protein ZapD -
  E5678_RS12055 (E5678_12055) - 2680591..2680794 (+) 204 WP_136178754.1 DNA gyrase inhibitor YacG -
  E5678_RS12060 (E5678_12060) - 2680811..2681245 (-) 435 WP_247597028.1 NUDIX domain-containing protein -
  E5678_RS12065 (E5678_12065) - 2681292..2682176 (-) 885 WP_136178756.1 ATP-binding protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44463.25 Da        Isoelectric Point: 9.5341

>NTDB_id=357633 E5678_RS12035 WP_136178750.1 2677111..2678328(+) (pilC) [Hydrogenophaga sp. PAMC20947]
MATAALKKVKDFVFEWEGKDRNGKQVRGETRAVGQNQVLAALRRQGIVPGKVKKRRMSSGKRIKPKDIAIFTRQLATMMK
AGVPLLQSFDIVGRGNPNPNVSKLLNDIRSDVETGTSLSGAFRKNPLYFDTLYCNLIEAGETAGILEELLDRLAVYMEKT
EAIKSKIKSALMYPISVVIVAFVVVAVIMIFVIPSFKEVFSSFGADLPGPTLFVIAISEFFTEYWWLIFGGIGGGLYFFF
QAWRRNEKMQRVMDRLMLKMPIFGTLVEKSVIARWTRTLATMFAAGVPLVEALDSVGGASGNSVYAMATERIQQEVSTGT
SLTNAMTNANLFPSMVLQMCAIGEESGSIDHMLGKAADFYEAEVDDMVAGISSLMEPIIIVVLGTIIGGIVVSMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=357633 E5678_RS12035 WP_136178750.1 2677111..2678328(+) (pilC) [Hydrogenophaga sp. PAMC20947]
ATGGCAACCGCAGCATTGAAAAAGGTGAAGGACTTTGTCTTCGAATGGGAAGGCAAAGACCGCAATGGCAAGCAGGTCCG
CGGGGAGACACGCGCAGTCGGCCAGAACCAGGTCTTGGCGGCTCTGCGCCGCCAGGGCATTGTGCCCGGCAAGGTCAAAA
AACGCCGCATGTCGTCGGGCAAGCGCATCAAGCCCAAAGACATCGCCATCTTCACGCGCCAGCTCGCCACCATGATGAAA
GCAGGCGTACCCCTGCTGCAATCGTTCGATATTGTGGGCCGCGGGAATCCCAATCCCAATGTATCCAAGCTGTTGAACGA
TATTCGTTCCGATGTTGAAACCGGCACCTCGCTCAGCGGCGCCTTCCGCAAAAACCCGCTGTACTTTGACACGCTGTATT
GCAACTTGATCGAAGCGGGAGAGACTGCCGGTATTCTGGAAGAGTTGCTGGACCGCCTGGCGGTCTACATGGAGAAGACC
GAGGCCATCAAGTCGAAAATCAAGTCGGCTTTGATGTACCCCATCTCCGTTGTTATTGTCGCCTTTGTGGTGGTGGCTGT
GATCATGATTTTTGTGATCCCTTCTTTCAAAGAGGTGTTCTCATCCTTCGGCGCGGACTTGCCCGGCCCAACATTGTTTG
TCATCGCCATCAGTGAGTTCTTCACGGAATACTGGTGGCTGATCTTTGGTGGCATCGGCGGGGGACTGTACTTTTTCTTC
CAGGCCTGGCGCCGAAACGAAAAGATGCAGCGGGTGATGGACCGGCTGATGCTGAAGATGCCCATTTTCGGCACATTGGT
GGAGAAATCTGTGATCGCTCGGTGGACCAGAACGCTGGCAACGATGTTTGCGGCGGGCGTGCCTTTGGTGGAGGCACTGG
ACTCTGTGGGGGGTGCATCGGGCAATTCGGTCTATGCCATGGCCACCGAAAGAATCCAGCAAGAAGTCTCCACCGGGACC
AGTCTCACCAACGCCATGACCAACGCCAACCTGTTTCCGTCCATGGTTTTGCAAATGTGCGCCATCGGTGAGGAGTCAGG
TTCCATCGATCACATGTTGGGCAAAGCTGCTGACTTCTATGAGGCAGAGGTTGACGACATGGTTGCCGGTATCTCCAGCT
TGATGGAACCCATCATCATCGTGGTTCTGGGAACCATCATTGGTGGCATCGTGGTCTCCATGTACCTGCCCATTTTCAAA
CTGGGTCAGGTTGTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P7RCP2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.703

99.753

0.546
  pilG Neisseria gonorrhoeae MS11

51.88

98.519

0.511
  pilG Neisseria meningitidis 44/76-A

51.378

98.519

0.506
  pilC Legionella pneumophila strain ERS1305867

50

98.272

0.491
  pilC Acinetobacter baylyi ADP1

49.75

98.765

0.491
  pilC Acinetobacter baumannii D1279779

48.12

98.519

0.474
  pilC Vibrio cholerae strain A1552

42

98.765

0.415
  pilC Vibrio campbellii strain DS40M4

38.916

100

0.39
  pilC Thermus thermophilus HB27

37.594

98.519

0.37

Multiple sequence alignment