Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E5Y90_RS12640 Genome accession   NZ_CP039143
Coordinates   2664648..2666363 (+) Length   571 a.a.
NCBI ID   WP_174660361.1    Uniprot ID   -
Organism   Acinetobacter sp. 10FS3-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2659648..2671363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5Y90_RS12595 (E5Y90_12640) nusA 2660238..2661722 (-) 1485 WP_151204969.1 transcription termination factor NusA -
  E5Y90_RS12600 (E5Y90_12645) rimP 2661758..2662282 (-) 525 WP_151204970.1 ribosome maturation factor RimP -
  E5Y90_RS12630 (E5Y90_12675) secG 2663233..2663562 (-) 330 WP_174660359.1 preprotein translocase subunit SecG -
  E5Y90_RS12635 (E5Y90_12680) tpiA 2663576..2664367 (-) 792 WP_174660360.1 triose-phosphate isomerase -
  E5Y90_RS12640 (E5Y90_12685) pilB 2664648..2666363 (+) 1716 WP_174660361.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E5Y90_RS12645 (E5Y90_12690) pilC 2666395..2667624 (+) 1230 WP_174660362.1 type II secretion system F family protein Machinery gene
  E5Y90_RS12650 (E5Y90_12695) pilD 2667624..2668484 (+) 861 WP_151204975.1 prepilin peptidase Machinery gene
  E5Y90_RS12655 (E5Y90_12700) coaE 2668486..2669103 (+) 618 WP_174660363.1 dephospho-CoA kinase -
  E5Y90_RS12660 (E5Y90_12705) - 2669325..2670248 (-) 924 WP_174660364.1 DMT family transporter -
  E5Y90_RS12665 (E5Y90_12710) rlmB 2670255..2671004 (-) 750 WP_174660365.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63776.05 Da        Isoelectric Point: 4.8060

>NTDB_id=357222 E5Y90_RS12640 WP_174660361.1 2664648..2666363(+) (pilB) [Acinetobacter sp. 10FS3-1]
MTVLQGTPRFTGFIRRLVDEGALSAEEMRTALDKAKQEKTDIVAYLIGHYNLSPTTIAETISVEFAEPLFDLGAYDSGQI
LREEIDEKLITKHRVLPIFKSANILYIATSNPTNIEAIDAIRFATKLNIESIIVEYNKLEKLIEQNFTEESTFDFDDSFD
LDLDVEGSEISDKEDEEDQEKGEEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRVDGVLRQIATPPLQLANRLA
SRLKVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKALFMAALEK
PQGMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCAQCKKPADIPKQSLLEMG
FTEEDLQHPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIEIAQASAHAGFNNLRRSGLMKVMQGVT
SLQEVNRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=357222 E5Y90_RS12640 WP_174660361.1 2664648..2666363(+) (pilB) [Acinetobacter sp. 10FS3-1]
ATGACAGTATTACAGGGGACCCCGAGATTTACGGGGTTTATTCGTCGCTTAGTCGATGAAGGTGCATTATCGGCTGAAGA
GATGCGGACTGCTTTGGACAAGGCCAAGCAGGAAAAAACGGATATCGTGGCGTATTTGATCGGCCACTATAATCTTTCTC
CAACGACAATTGCCGAAACAATTTCAGTTGAATTTGCCGAGCCTTTATTTGATCTGGGGGCGTATGATTCTGGACAAATC
TTACGTGAAGAAATAGATGAAAAATTAATTACCAAACACCGGGTTCTGCCAATTTTTAAGAGTGCCAATATCCTTTATAT
TGCCACCAGTAACCCGACCAATATTGAAGCCATTGATGCAATCCGCTTTGCCACCAAACTGAATATTGAAAGTATTATTG
TTGAATACAATAAACTGGAAAAACTGATTGAACAGAATTTCACCGAAGAAAGCACGTTTGATTTTGATGACAGTTTTGAT
CTCGATCTTGATGTCGAAGGCAGTGAAATCTCGGATAAAGAAGACGAGGAAGACCAGGAAAAAGGCGAAGAAGCTCCTAT
TGTTAAATATATTAATAAATTGCTGATTGATGCAATCCGGATGGGTGCATCGGATCTGCATTTTGAGCCTTATGAAAAGA
TATACCGGGTTCGTTACCGAGTCGATGGGGTGCTGCGTCAAATTGCCACGCCACCTTTGCAATTAGCTAACCGTTTAGCC
TCCCGCTTAAAAGTCATGTCACAAATGGATATTTCTGAAAAGCGTATTCCCCAAGATGGCCGTATCAAATTAAAGCTCTC
CAAAAATAAAGCAATCGACTTCCGTGTGAACTCACTGCCTACCCTGTTTGGGGAAAAACTGGTACTGCGTATTCTGGATC
CGTCCAGTGCAATGCTGGGAATTGATGCCTTGGGTTATGAGCCTGAACAGAAAGCCCTGTTTATGGCAGCCTTGGAGAAA
CCTCAAGGCATGCTATTGATTACCGGTCCTACCGGTTCGGGTAAAACCGTTTCCCTGTATACCGGCCTGAATATCTTAAA
CCGTGAAGATACCAATATTTCAACGGCCGAAGATCCGGTCGAGATTAACCTGCAAGGGATTAACCAGGTCAACGTCAACA
ACAAGGTCGGCCTGACCTTTTCCGCTGCACTTAAATCCTTCCTGCGTCAGGACCCGGATATTGTCATGGTCGGTGAGATT
CGTGATCTGGAAACGGCTGAAATTGCCATCAAGGCGGCACAAACCGGACATATGGTCATGTCCACACTGCACACCAACAG
TGCACCGGAAACCCTGACTCGTTTACGCAATATGGGAGTTCCTTCTTTTAATATTGCAACCTCAGTCAACCTAGTCATTG
CCCAGCGTCTGGCCCGCCGTCTATGCGCACAATGTAAAAAACCAGCCGATATTCCGAAGCAGAGCCTCTTGGAGATGGGC
TTTACCGAAGAAGATCTCCAACACCCGGAATTTCAGATTTATGAACCGGTTGGCTGCAATGAATGTCGAGAAGGCTATAA
AGGTCGTGTCGGCATTTATGAAGTTATGAAAGTAACACCAGAAATTTCAAGAATTATTATGGAAGATGGCAATGCAATTG
AAATTGCTCAGGCTTCTGCACATGCAGGTTTTAACAACTTACGTCGATCAGGTTTAATGAAGGTTATGCAGGGGGTGACG
TCCTTACAGGAAGTCAATCGTGTAACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.42

100

0.806
  pilB Acinetobacter baylyi ADP1

76.182

100

0.762
  pilB Legionella pneumophila strain ERS1305867

51.767

99.124

0.513
  pilF Neisseria gonorrhoeae MS11

48.845

98.599

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

47.772

98.249

0.469
  pilB Vibrio campbellii strain DS40M4

47.653

97.023

0.462
  pilB Vibrio cholerae strain A1552

50.579

90.718

0.459
  pilF Thermus thermophilus HB27

36.632

100

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

33.987

100

0.364

Multiple sequence alignment