Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   E5Y90_RS12650 Genome accession   NZ_CP039143
Coordinates   2667624..2668484 (+) Length   286 a.a.
NCBI ID   WP_151204975.1    Uniprot ID   -
Organism   Acinetobacter sp. 10FS3-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2662624..2673484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5Y90_RS12630 (E5Y90_12675) secG 2663233..2663562 (-) 330 WP_174660359.1 preprotein translocase subunit SecG -
  E5Y90_RS12635 (E5Y90_12680) tpiA 2663576..2664367 (-) 792 WP_174660360.1 triose-phosphate isomerase -
  E5Y90_RS12640 (E5Y90_12685) pilB 2664648..2666363 (+) 1716 WP_174660361.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E5Y90_RS12645 (E5Y90_12690) pilC 2666395..2667624 (+) 1230 WP_174660362.1 type II secretion system F family protein Machinery gene
  E5Y90_RS12650 (E5Y90_12695) pilD 2667624..2668484 (+) 861 WP_151204975.1 prepilin peptidase Machinery gene
  E5Y90_RS12655 (E5Y90_12700) coaE 2668486..2669103 (+) 618 WP_174660363.1 dephospho-CoA kinase -
  E5Y90_RS12660 (E5Y90_12705) - 2669325..2670248 (-) 924 WP_174660364.1 DMT family transporter -
  E5Y90_RS12665 (E5Y90_12710) rlmB 2670255..2671004 (-) 750 WP_174660365.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  E5Y90_RS12670 (E5Y90_12715) - 2671147..2671470 (-) 324 WP_151205572.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32121.45 Da        Isoelectric Point: 8.0681

>NTDB_id=357224 E5Y90_RS12650 WP_151204975.1 2667624..2668484(+) (pilD) [Acinetobacter sp. 10FS3-1]
MQDLIFYFSHNPTALYLAVGLFSLCIGSFLNVVIYRTPKMMEQEWRTDCQLFLHPDQPIIDESKITLSKPASTCPKCQTP
IRWYQNIPVMSWILLRGKCGNCKHPISIRYPFIELLTAICALIVVAVFGPTLQMLFGLVLTYVLITLTFIDFDTQLLPDR
YTLPLAALGLGINSYAIYTSASSAIWGYILGFLCLWVVYYLFKVVTGKEGMGYGDFKLLAALGAWMGPLLLPLIVLLSSL
VGAIIGIILLKIRKENQPFAFGPYIAIAGWIAFLWGEQIMHIYLGQ

Nucleotide


Download         Length: 861 bp        

>NTDB_id=357224 E5Y90_RS12650 WP_151204975.1 2667624..2668484(+) (pilD) [Acinetobacter sp. 10FS3-1]
ATGCAAGACCTTATTTTTTATTTTTCTCACAATCCGACTGCGCTTTATCTCGCAGTTGGGCTTTTTAGTCTCTGTATCGG
CAGTTTTTTAAATGTGGTGATCTATCGCACGCCAAAAATGATGGAACAGGAGTGGCGTACTGACTGTCAGCTGTTCCTGC
ATCCTGATCAGCCCATCATTGATGAAAGTAAAATCACCCTCAGCAAACCCGCCTCAACCTGTCCAAAATGTCAGACGCCA
ATTCGCTGGTATCAAAATATTCCAGTGATGAGCTGGATATTATTGCGTGGTAAATGTGGTAACTGCAAACATCCAATCAG
TATTCGCTATCCTTTTATAGAACTGTTAACTGCGATTTGTGCACTGATCGTTGTGGCAGTATTTGGTCCTACCCTACAGA
TGCTCTTTGGCCTGGTATTAACCTATGTGCTGATTACCCTGACTTTTATTGATTTTGATACCCAACTCTTACCCGACCGT
TACACCTTGCCACTGGCAGCGCTGGGCCTCGGGATTAATAGTTATGCCATCTATACCTCCGCAAGTTCGGCCATCTGGGG
GTATATTCTGGGCTTTTTATGTTTGTGGGTGGTGTATTACCTGTTTAAAGTGGTGACGGGCAAGGAAGGCATGGGCTATG
GAGACTTTAAGCTGCTGGCAGCTTTAGGTGCCTGGATGGGCCCCCTGCTTTTACCGCTGATTGTGTTATTGTCTTCTTTG
GTCGGGGCAATTATCGGGATTATTCTGCTCAAGATCCGTAAAGAAAATCAGCCCTTTGCTTTTGGTCCTTATATTGCCAT
TGCTGGCTGGATCGCCTTTTTATGGGGCGAACAGATCATGCACATTTATTTAGGTCAGTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

80.351

99.65

0.801
  pilD Acinetobacter nosocomialis M2

80

99.65

0.797
  pilD Vibrio cholerae strain A1552

53.191

98.601

0.524
  pilD Vibrio campbellii strain DS40M4

51.254

97.552

0.5
  pilD Neisseria gonorrhoeae MS11

47.232

94.755

0.448

Multiple sequence alignment