Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   E4187_RS19135 Genome accession   NZ_CP038445
Coordinates   4133497..4134936 (-) Length   479 a.a.
NCBI ID   WP_041204880.1    Uniprot ID   -
Organism   Aeromonas media strain R25-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4128497..4139936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4187_RS19105 (E4187_19320) degS 4129240..4130361 (+) 1122 WP_041204883.1 outer membrane-stress sensor serine endopeptidase DegS -
  E4187_RS23270 (E4187_19325) - 4130476..4131521 (+) 1046 Protein_3717 PTS sugar transporter subunit IIA -
  E4187_RS19115 (E4187_19330) npr 4131540..4131812 (-) 273 WP_005331359.1 PTS phosphocarrier protein NPr -
  E4187_RS19120 (E4187_19335) rapZ 4131833..4132699 (-) 867 WP_041204881.1 RNase adapter RapZ -
  E4187_RS19125 (E4187_19340) ptsN 4132740..4133186 (-) 447 WP_005331355.1 PTS IIA-like nitrogen regulatory protein PtsN -
  E4187_RS19130 (E4187_19345) hpf 4133189..4133476 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  E4187_RS19135 (E4187_19350) rpoN/rpoN1 4133497..4134936 (-) 1440 WP_041204880.1 RNA polymerase factor sigma-54 Machinery gene
  E4187_RS19140 (E4187_19355) lptB 4135002..4135727 (-) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  E4187_RS19145 (E4187_19360) lptA 4135740..4136258 (-) 519 WP_042649049.1 lipopolysaccharide transport periplasmic protein LptA -
  E4187_RS19150 (E4187_19365) lptC 4136245..4136805 (-) 561 WP_042649050.1 LPS export ABC transporter periplasmic protein LptC -
  E4187_RS19155 (E4187_19370) kdsC 4136802..4137356 (-) 555 WP_042649051.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  E4187_RS19160 (E4187_19375) - 4137356..4138351 (-) 996 WP_025328534.1 KpsF/GutQ family sugar-phosphate isomerase -
  E4187_RS19165 (E4187_19380) mlaF 4138558..4139358 (+) 801 WP_025328535.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53771.74 Da        Isoelectric Point: 4.4457

>NTDB_id=354424 E4187_RS19135 WP_041204880.1 4133497..4134936(-) (rpoN/rpoN1) [Aeromonas media strain R25-3]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAHETSPIEPQDASQLDSSDAMAQDKMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDESGYLTVSLED
IQAAVSSEEVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGAKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=354424 E4187_RS19135 WP_041204880.1 4133497..4134936(-) (rpoN/rpoN1) [Aeromonas media strain R25-3]
ATGAAGCCGACATTACAGTTGCGTCTCGGCCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAATAACCCCTTGCTGGAGCAGGAGGAGAATGAGG
CGCACGAAACCAGTCCGATAGAGCCCCAGGACGCCAGTCAACTCGACTCCAGTGACGCAATGGCACAGGACAAGATGCCG
GATGAACTGCCGGTGGACACTACCTGGGATGAGGTCTATTCCGCTGGCACGGCTCAGAGCGCTGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAAACCACCGAAAATCTGCAGGATTATCTGTTGTGGCAGATGCGACTGACGCCGTTCA
GTGATCTGGATGCCGCGATTGCCCTCGCCATCATCGATGCCATCGATGAGTCTGGCTACCTGACCGTTTCTCTCGAAGAT
ATTCAGGCTGCAGTCAGCAGTGAAGAGGTAGAAGTTGAACTGGATGAAGTGGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTTGGCATCGCCGCTCGCTCGGTACAGGAGTGTCTGTTGATCCAGCTTGGCCAGTATTCGCCGGATCTGCCCT
GGCTCAATGAGGCAAGGGAGGTGATCCGCGAGCACATGGACTTGCTCGGCAACCGTGACTACCGCACCCTGGCGCGCAAG
ACCCGGCTCAAGGAGAGCGACCTCAAGGAGGTACTGGATCTCATCCAGCAGCTGGAGCCCAGACCCGGTAATGGCATCAT
CCAGAGCGAATCGCAATACGTGATCCCGGATGTTTCCGTGGTCAAGAAGGGGGCGAAGTGGGTGGTTGAACTCAACCCGG
ATTCGGCGCCGCGCATTCGTGTCAACGAGGCCTATGCGGCAATGGCACGCAATGCCCGCAGCAGCACGGATACTCAGTTC
ATTCGTTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGAAACGACACCCTGCTCAAGGTGGCAAG
CTGCATAGTCGAGCAGCAACAGGGTTTCTTCGAGTACGGTGAAGAGGCCATGAAGCCCATGGTACTCAACGACATCGCCG
AGGCGGTGGAGATGCACGAGTCGACGATTTCACGGGTGACGACCCAGAAGTACATGCATACGCCCCGTGGCATCTTTGAA
CTGAAGTTTTTTTTCTCCAGTCATGTCAACACGGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCCGAAAACCCGGCCAAACCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.667

100

0.497

Multiple sequence alignment