Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   E4187_RS18910 Genome accession   NZ_CP038445
Coordinates   4090587..4091828 (+) Length   413 a.a.
NCBI ID   WP_042649026.1    Uniprot ID   A0AAE7ADJ1
Organism   Aeromonas media strain R25-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4085587..4096828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4187_RS18890 (E4187_19100) - 4086491..4087630 (-) 1140 WP_041206992.1 RNA-guided endonuclease TnpB family protein -
  E4187_RS18895 (E4187_19105) tnpA 4087688..4088113 (+) 426 WP_041205574.1 IS200/IS605-like element ISAeme8 family transposase -
  E4187_RS23030 - 4088286..4088453 (+) 168 Protein_3676 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  E4187_RS18905 (E4187_19120) pilB 4088751..4090457 (+) 1707 WP_042649025.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  E4187_RS18910 (E4187_19125) pilC 4090587..4091828 (+) 1242 WP_042649026.1 type II secretion system F family protein Machinery gene
  E4187_RS18915 (E4187_19130) pilD 4091909..4092781 (+) 873 WP_042649027.1 A24 family peptidase Machinery gene
  E4187_RS18920 (E4187_19135) coaE 4092800..4093414 (+) 615 WP_042649028.1 dephospho-CoA kinase -
  E4187_RS18925 (E4187_19140) zapD 4093452..4094174 (+) 723 WP_042649029.1 cell division protein ZapD -
  E4187_RS18930 (E4187_19145) yacG 4094184..4094378 (+) 195 WP_042649030.1 DNA gyrase inhibitor YacG -
  E4187_RS18935 (E4187_19150) mutT 4094454..4094858 (-) 405 WP_005332118.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45507.55 Da        Isoelectric Point: 10.0692

>NTDB_id=354422 E4187_RS18910 WP_042649026.1 4090587..4091828(+) (pilC) [Aeromonas media strain R25-3]
MATLAQKRNTPKKVFSFRWHGFNRKGQKVSGEFQADSINTVKAELRKQGVNVTKVSKQGQGLFSKGGARIKPMDIAVISR
QITTMLSAGVPLVQSLQIIARGHEKAAVRELIGQIATDVETGTPLSEALRRHPRHFDALYCDLVDAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPAMVILVAIIVTSILLLFVIPQFEEIFKSFGAELPVFTQFVIGISRFMQAWWYIIFGGTA
LAIFLYVRAWRKSQKVRDNTDRFILTIPVVGNILHKAAMARFARTLSTTFSAGIPLVDALVSAAGASGNYVYRTAILAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVAAIFEQEVDDMVDGLTSLLEPIIMVVLGVLVGGMVVA
MYLPIFKLGSVIH

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=354422 E4187_RS18910 WP_042649026.1 4090587..4091828(+) (pilC) [Aeromonas media strain R25-3]
ATGGCAACATTAGCGCAAAAACGTAACACACCTAAAAAGGTCTTCTCCTTCCGCTGGCATGGATTCAATCGCAAGGGACA
AAAGGTTTCCGGTGAGTTCCAGGCCGACAGCATCAACACCGTCAAGGCGGAGCTGCGCAAGCAGGGCGTCAATGTCACCA
AGGTCAGCAAGCAGGGCCAGGGCCTGTTTTCCAAGGGGGGTGCTCGGATCAAGCCGATGGACATCGCCGTCATCTCCCGT
CAGATCACCACCATGCTCTCGGCCGGTGTGCCGCTGGTGCAAAGCTTGCAGATCATCGCCCGCGGTCATGAGAAGGCAGC
AGTACGCGAACTCATCGGCCAGATCGCCACCGATGTGGAAACCGGCACCCCGCTGTCAGAGGCGCTGCGCCGCCATCCCC
GCCACTTCGATGCGCTCTACTGCGACCTGGTAGACGCGGGGGAGCAGTCCGGCGCGCTGGAAACCATCTACGACCGGATT
GCCACATACCGCGAGAAGAGTGAGGCGCTGAAGTCCAAGATCAAGAAAGCCATGTTCTATCCCGCCATGGTCATCCTGGT
CGCCATCATCGTAACCTCCATCCTGCTGCTGTTCGTCATTCCCCAGTTCGAGGAGATCTTCAAGAGCTTCGGGGCAGAGC
TGCCAGTCTTCACCCAATTCGTCATCGGTATCTCCCGCTTCATGCAAGCTTGGTGGTATATCATCTTTGGTGGCACTGCC
TTGGCCATCTTCCTCTATGTGCGCGCCTGGCGAAAATCCCAGAAGGTGCGGGACAACACCGACAGATTCATCCTCACCAT
TCCTGTCGTGGGCAACATATTGCACAAGGCGGCCATGGCCCGCTTCGCCCGTACCCTCTCCACCACCTTCTCCGCCGGGA
TACCTCTGGTGGATGCCCTGGTGTCAGCGGCCGGCGCCTCTGGCAACTATGTCTATCGCACGGCCATCCTGGCCATTCGC
AACGAAGTGGTGGCCGGCATGCAGATCAACGTCGCCATGCGTACCGTGGATCTCTTCCCAGACATGGTGATCCAGATGGT
GATGATTGGTGAAGAGTCAGGCGCCATCGATGACATGCTCTCCAAGGTTGCCGCCATCTTCGAGCAGGAGGTGGACGACA
TGGTCGATGGCCTCACCAGCCTGCTCGAACCCATCATCATGGTGGTGCTCGGGGTGCTGGTCGGTGGCATGGTCGTCGCC
ATGTACCTCCCCATCTTCAAGCTGGGCTCTGTGATACACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.818

95.884

0.545
  pilC Legionella pneumophila strain ERS1305867

52.84

98.063

0.518
  pilC Acinetobacter baumannii D1279779

51.372

97.094

0.499
  pilC Acinetobacter baylyi ADP1

50.882

96.126

0.489
  pilC Vibrio cholerae strain A1552

48.25

96.852

0.467
  pilC Vibrio campbellii strain DS40M4

46.734

96.368

0.45
  pilG Neisseria gonorrhoeae MS11

40.299

97.337

0.392
  pilG Neisseria meningitidis 44/76-A

40.05

97.337

0.39
  pilC Thermus thermophilus HB27

38.75

96.852

0.375

Multiple sequence alignment