Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E4185_RS08615 Genome accession   NZ_CP038443
Coordinates   1801929..1803635 (-) Length   568 a.a.
NCBI ID   WP_171273132.1    Uniprot ID   -
Organism   Aeromonas media strain R1-18     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1796929..1808635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4185_RS08585 (E4185_08650) mutT 1797528..1797932 (+) 405 WP_043133317.1 8-oxo-dGTP diphosphatase MutT -
  E4185_RS08590 (E4185_08655) yacG 1798008..1798202 (-) 195 WP_042649030.1 DNA gyrase inhibitor YacG -
  E4185_RS08595 (E4185_08660) zapD 1798212..1798934 (-) 723 WP_161507570.1 cell division protein ZapD -
  E4185_RS08600 (E4185_08665) coaE 1798972..1799586 (-) 615 WP_171273130.1 dephospho-CoA kinase -
  E4185_RS08605 (E4185_08670) pilD 1799605..1800477 (-) 873 WP_171273131.1 A24 family peptidase Machinery gene
  E4185_RS08610 (E4185_08675) pilC 1800558..1801799 (-) 1242 WP_043133310.1 type II secretion system F family protein Machinery gene
  E4185_RS08615 (E4185_08680) pilB 1801929..1803635 (-) 1707 WP_171273132.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  E4185_RS08620 (E4185_08685) - 1803639..1804061 (-) 423 WP_171274319.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  E4185_RS08625 (E4185_08695) nadC 1804386..1805249 (-) 864 WP_171273133.1 carboxylating nicotinate-nucleotide diphosphorylase -
  E4185_RS08630 (E4185_08700) - 1805253..1805723 (-) 471 WP_042648139.1 retropepsin-like aspartic protease -
  E4185_RS08635 (E4185_08705) ampD 1805860..1806432 (+) 573 WP_043128627.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  E4185_RS08640 (E4185_08710) pdhR 1806709..1807473 (+) 765 WP_005329211.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62653.08 Da        Isoelectric Point: 5.8224

>NTDB_id=354363 E4185_RS08615 WP_171273132.1 1801929..1803635(-) (pilB) [Aeromonas media strain R1-18]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEEGKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDDSVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKVVIRLLDSSVSRLNLDQLGFDERQKAQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKVPEAVPEAELLEFGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=354363 E4185_RS08615 WP_171273132.1 1801929..1803635(-) (pilB) [Aeromonas media strain R1-18]
ATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCTTGGCCGCTTCCTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATCGAAAACGACATCCTCGACAGCAAGGCCCTCG
CGGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAATTGATTGAGAAGCACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCTCTGGAGGATTTTGGCTTCAGCTTCGGCCTGCATACCGAAGCCTTGCTGGTCGAAGAGGGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAAAGCGAGCAGGACACTCTGGGCATGGATCACATCGACGAGGCCGAAATC
TCGGAACTCGAGGTCTCCGATGAAAGCTCGCGGCTCGACGACAGCGTCAACACTGCCGACGACGATGCCCCCATCGTCAA
ATACATTCACAAGATCATGATGGACGCCATCAAACGTGGCGCATCCGACCTGCATTTCGAACCCTATGAGACCAAGTACC
GGATCCGCTTCCGGATCGACGGCATACTGCACGAAATCGCCACCCCGCCGGTCAATCTGGCGAACCGTTTCGCGGCTCGC
TTAAAGGTAATGGCGCGGCTCGATATCGCCGAGCGGCGCCTGCCCCAGGATGGACGGATCAAGCTGAAGATCTCCCGCAA
CCGGTCCATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAAAAGGTCGTCATTCGCCTGCTGGACTCGTCAG
TATCTCGTCTCAACCTCGACCAACTCGGCTTCGACGAACGCCAGAAGGCGCAATATCTGCATGCGCTCTCCAAGCCACAG
GGCATGATACTGGTCACGGGCCCCACGGGATCGGGCAAGACAGTATCACTCTATACCGGTCTCAACATCCTCAATACCAG
CGAGGTCAACATCTCCACCGCCGAGGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCTGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAGGCCGCTCAGACCGGACACCTTGTGCTGTCAACCCTGCACACCAACTCGGCGGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTAACCCTGATCATGGCCCAGC
GCCTCGCCCGCAAGCTGTGCGATCACTGCAAGGTGCCCGAGGCGGTGCCGGAAGCCGAATTGCTGGAGTTCGGTTTTACC
CAGCAGCAGCTGGCCGCCGGTTTGCGGCTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCGGGAGGATACAGAGGACGGGT
CGGCATCTATGAAATCATGCTGATGTCGGAGAACATCGCCAAGCTGATCATGCAGGGAGCAAACTCGCTGCAGATCGCTG
CCATCGCCCAGAAGGAGGGTATGCGTACCCTGCGCATTTCCGGCCTGGAGAAGGCCCGTCTGGGGGTCACCAGCCTGGCC
GAGATCAACAGGATCACGACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.674

99.824

0.546
  pilB Acinetobacter baylyi ADP1

54.707

99.12

0.542
  pilB Legionella pneumophila strain ERS1305867

53.901

99.296

0.535
  pilB Vibrio cholerae strain A1552

53.014

99.296

0.526
  pilB Vibrio parahaemolyticus RIMD 2210633

49.556

99.12

0.491
  pilB Vibrio campbellii strain DS40M4

49.556

99.12

0.491
  pilF Neisseria gonorrhoeae MS11

46.032

99.824

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

38.225

97.183

0.371
  pilF Thermus thermophilus HB27

39.736

93.486

0.371

Multiple sequence alignment