Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E4184_RS19740 Genome accession   NZ_CP038441
Coordinates   4265994..4267700 (+) Length   568 a.a.
NCBI ID   WP_171276943.1    Uniprot ID   -
Organism   Aeromonas media strain T0.1-19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4260994..4272700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4184_RS19715 (E4184_19870) pdhR 4262142..4262906 (-) 765 WP_106885661.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  E4184_RS19720 (E4184_19875) ampD 4263182..4263754 (-) 573 WP_171276939.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  E4184_RS19725 (E4184_19880) - 4263891..4264361 (+) 471 WP_171276940.1 retropepsin-like aspartic protease -
  E4184_RS19730 (E4184_19885) nadC 4264365..4265228 (+) 864 WP_171276941.1 carboxylating nicotinate-nucleotide diphosphorylase -
  E4184_RS19735 (E4184_19895) - 4265541..4265990 (+) 450 WP_171276942.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  E4184_RS19740 (E4184_19900) pilB 4265994..4267700 (+) 1707 WP_171276943.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  E4184_RS19745 (E4184_19905) pilC 4267831..4269072 (+) 1242 WP_106885656.1 type II secretion system F family protein Machinery gene
  E4184_RS19750 (E4184_19910) pilD 4269153..4270025 (+) 873 WP_171276944.1 A24 family peptidase Machinery gene
  E4184_RS19755 (E4184_19915) coaE 4270044..4270658 (+) 615 WP_171276945.1 dephospho-CoA kinase -
  E4184_RS19760 (E4184_19920) zapD 4270696..4271418 (+) 723 WP_162520171.1 cell division protein ZapD -
  E4184_RS19765 (E4184_19925) yacG 4271428..4271622 (+) 195 WP_025328449.1 DNA gyrase inhibitor YacG -
  E4184_RS19770 (E4184_19930) mutT 4271697..4272101 (-) 405 WP_106885652.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62580.93 Da        Isoelectric Point: 5.8194

>NTDB_id=354338 E4184_RS19740 WP_171276943.1 4265994..4267700(+) (pilB) [Aeromonas media strain T0.1-19]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLSEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAALGKLMDTEQDTLGMDHIDEAEI
SELEVSDEGSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRSRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNLDQLGFDDRQKTQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPDAELQELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=354338 E4184_RS19740 WP_171276943.1 4265994..4267700(+) (pilB) [Aeromonas media strain T0.1-19]
ATGACCTCTAGCCTCAATAGCGGCCTGGCGACCAGCCTGGCCGCCTCTTCCCTCCTCAGCGAGCCGGATTCACAACGCTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATCGAGAACGACATCCTCGACAGCAAAGCGCTGG
CGGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTCTCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTTATCGAGAAACATCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCCCTGGAGGATTTCGGCTTCAGCTTCGGCCTGCACACTGAAGCCTTGCTGGTCGAGGAGAGCA
AGCTCACCGCCGCCCTCGGCAAGCTGATGGACACCGAGCAGGACACCCTGGGCATGGATCACATCGACGAGGCCGAGATC
TCGGAGCTCGAGGTGTCCGATGAGGGTTCGCGCCTCGACGAGAGCGTCAATACTGCCGACGACGATGCCCCCATAGTCAA
ATACATCCATAAAATCATGATGGATGCCATCAAGCGTGGCGCATCCGACCTGCATTTCGAACCCTATGAGACCAAGTACA
GGATCCGCTTCCGGATCGACGGCATACTGCATGAAGTCGCGACCCCACCGGTCAATCTGGCGAACCGTTTCGCGGCCCGT
CTCAAGGTGATGGCGAGACTCGATATCGCCGAGCGACGCCTGCCCCAGGATGGACGGATCAAGCTGAAGATCTCACGCAG
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAAATAGTCATCCGCTTGCTGGACTCGTCGG
CAGCCCGCCTCAACCTCGATCAGCTTGGCTTCGACGACCGCCAGAAGACGCAATACCTGCATGCGCTCTCTAAGCCACAG
GGCATGATACTGGTTACGGGGCCCACGGGGTCAGGAAAGACGGTGTCGCTCTATACAGGTCTCAATATCCTTAATACCAG
CGAGGTCAACATCTCCACCGCCGAGGACCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CTGGGCTTACCTTCGCCAGTGCCCTGCGCTCCTTCCTACGCCAGGATCCCGACATCGTCATGGTGGGGGAAATACGGGAT
CTGGAAACCGCCGAGATTGCCATCAAGGCCGCCCAGACCGGTCACCTGGTACTATCAACCCTGCACACCAACTCGGCGGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTCCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GTCTCGCCCGCAAGCTGTGCGATCACTGCAAGGCCCCTGAGGTGGTGCCCGACGCCGAACTGCAGGAGCTGGGCTTTACC
CAGCAGCAATTGGCCGCCGGACTGCGACTGTTCAAGCCGGTCGGCTGCAAGGAGTGTTCAGGAGGCTACAGAGGACGGGT
CGGCATCTATGAAATCATGCTGATGTCGGACAACATCGCCAAGCTGATCATGCAGGGGGCTAATTCGCTACAAATCGCCG
CCATCGCCCAGAAGGAGGGCATGCGCACCCTGCGCATCTCCGGCCTTGAGAAGGCTCGCCTGGGGGTCACCAGCCTGGCC
GAGATCAACAGGATCACCACGAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

54.947

99.648

0.548
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Acinetobacter baylyi ADP1

54.626

98.944

0.54
  pilB Vibrio cholerae strain A1552

52.931

99.12

0.525
  pilB Vibrio campbellii strain DS40M4

50.177

99.648

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

49.556

99.12

0.491
  pilF Neisseria gonorrhoeae MS11

46.384

99.824

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

38.087

97.535

0.371
  pilF Thermus thermophilus HB27

39.962

92.958

0.371

Multiple sequence alignment