Detailed information NTG0354283

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: E4T63_RS15225
• Genome accession: NZ_CP038438
• Coordinates: 3332853..3333965 (-)
• Length: 370 a.a.
• NCBI ID: WP_135296933.1
• Uniprot ID: A0AAP9CLE3
• Organism: Pseudomonas fluorescens strain LBUM677
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3327853..3338965

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E4T63_RS15210 (E4T63_15210)	-	3328281..3329672 (+)	1392	WP_027612663.1	GntP family permease	-
E4T63_RS15215 (E4T63_15215)	hbdH	3329680..3330453 (+)	774	WP_047294787.1	3-hydroxybutyrate dehydrogenase	-
E4T63_RS15220 (E4T63_15220)	-	3330593..3332548 (+)	1956	WP_135295907.1	acetoacetate--CoA ligase	-
E4T63_RS15225 (E4T63_15225)	pilU	3332853..3333965 (-)	1113	WP_135296933.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
E4T63_RS15230 (E4T63_15230)	-	3334099..3336648 (+)	2550	WP_135295908.1	PAS domain-containing protein	-
E4T63_RS15235 (E4T63_15235)	-	3336688..3336963 (+)	276	WP_003225447.1	peptidylprolyl isomerase	-
E4T63_RS15240 (E4T63_15240)	-	3337079..3338011 (-)	933	WP_135295909.1	sugar kinase	-

Sequence

Protein

• Length: 370 a.a.
• Molecular weight: 41153.04 Da
• Isoelectric Point: 6.6254

>NTDB_id=354283 E4T63_RS15225 WP_135296933.1 3332853..3333965(-) (pilU) [Pseudomonas fluorescens strain LBUM677]
MEIDALLLRLSSQHGSDLFLSTGAPPSARIDGVLTPFSERPFKPGEVETIANSLMDAEQRREFDRDLEMNLAISRTGIGR
FRVNVFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLETVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PVEYIHRHQRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALTFADTGHLVLSTLHAHNANQALDRI
VNLFPEERRPQLLHALGNNLKAFVSQRLVRTLDGQRRAAVEVMLGTPTIADLIRRNELGELKGIMEKSGEVGMQTFDAAL
YGLVVEGVISEDEALKHADSQNNLRLRLKLHAEAGPAPAPITSGDWGLMD

Nucleotide

• Length: 1113 bp

>NTDB_id=354283 E4T63_RS15225 WP_135296933.1 3332853..3333965(-) (pilU) [Pseudomonas fluorescens strain LBUM677]
ATGGAAATCGATGCACTGTTGCTCCGTCTGTCGAGCCAGCACGGTTCTGACCTGTTCCTGTCCACGGGAGCGCCACCCAG
TGCGCGCATCGATGGCGTGCTGACGCCGTTCAGCGAGCGGCCGTTCAAGCCCGGCGAGGTCGAGACTATCGCCAACTCCC
TGATGGACGCCGAGCAACGGCGCGAGTTCGATCGGGATCTGGAAATGAACCTGGCGATTTCCCGTACGGGCATCGGGCGT
TTTCGGGTGAACGTCTTCAAGCAACGCAATGATGTGTCAATCGTAATCCGCAACGTCAAACTCGATATCCCGCGCTTCGA
AGACCTGAAACTGCCGCCGGTATTGCTGGAAACGGTGATGCTCAAACAGGGGCTGATCCTGTTCGTTGGCGCCACCGACT
CCGGCAAGTCCACTTCGCTGGCCGCGCTGATCGATCACCGCAACCGCCACAGCAGCGGGCACATCATCACCATCGAAGAC
CCGGTGGAGTATATCCATCGACACCAGCGCTCGATCATCAATCAACGCGAAGTCGGCGTCGATACCCGCAGCTTCCATGC
GGCGCTGAAAAACACCCTGCGCCAGGCCCCGGATGTGGTGCTGATCGGCGAGATCCGCGACCGCGAAACCATGGAGCATG
CGCTGACATTTGCCGACACCGGGCACCTGGTGCTGTCGACGTTGCATGCGCACAATGCCAATCAGGCGCTGGACCGTATC
GTCAATCTGTTCCCCGAAGAGCGCCGCCCGCAGTTGCTGCATGCACTGGGCAACAATCTCAAGGCGTTCGTCTCGCAACG
GCTGGTGCGTACGCTGGACGGTCAGCGCCGGGCGGCGGTCGAGGTGATGCTTGGCACCCCGACCATCGCCGACCTGATCC
GCCGCAACGAGTTGGGCGAACTCAAGGGCATCATGGAAAAGTCCGGCGAGGTCGGCATGCAGACCTTCGACGCGGCGCTG
TACGGGCTGGTGGTGGAGGGCGTGATCAGTGAGGACGAAGCACTCAAACATGCCGATTCGCAGAACAACCTGCGCCTGCG
TTTGAAGCTGCACGCCGAGGCGGGCCCGGCGCCCGCGCCCATTACATCAGGCGATTGGGGCCTGATGGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (69-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.62	95.946	0.543
pilU	Acinetobacter baylyi ADP1	53.221	96.486	0.514
pilU	Vibrio cholerae strain A1552	52.857	94.595	0.5
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.223	96.757	0.389
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	39.595	93.514	0.37
pilT	Vibrio cholerae strain A1552	39.595	93.514	0.37
pilT	Pseudomonas stutzeri DSM 10701	39.535	92.973	0.368
pilT	Pseudomonas aeruginosa PAK	39.244	92.973	0.365