Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EXH44_RS01975 Genome accession   NZ_CP038145
Coordinates   422726..424111 (+) Length   461 a.a.
NCBI ID   WP_162856043.1    Uniprot ID   A0A4P7CE13
Organism   Actinobacillus indolicus strain AIFJ1607     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417726..429111
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXH44_RS01960 (EXH44_01975) ppc 417944..420583 (+) 2640 WP_162856041.1 phosphoenolpyruvate carboxylase -
  EXH44_RS01965 (EXH44_01980) radA 420650..422026 (-) 1377 WP_162856042.1 DNA repair protein RadA -
  EXH44_RS01970 (EXH44_01985) pilA 422214..422678 (+) 465 WP_071610760.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  EXH44_RS01975 (EXH44_01990) pilB 422726..424111 (+) 1386 WP_162856043.1 GspE/PulE family protein Machinery gene
  EXH44_RS01980 (EXH44_01995) pilC 424104..425300 (+) 1197 WP_162856044.1 type II secretion system F family protein Machinery gene
  EXH44_RS01985 (EXH44_02000) - 425297..425965 (+) 669 WP_162856045.1 prepilin peptidase -
  EXH44_RS01990 (EXH44_02005) coaE 426063..426689 (+) 627 WP_162856046.1 dephospho-CoA kinase -
  EXH44_RS01995 (EXH44_02010) yacG 426686..426865 (+) 180 WP_162856047.1 DNA gyrase inhibitor YacG -
  EXH44_RS02000 (EXH44_02015) lpxA 426916..427710 (-) 795 WP_162856048.1 acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase -
  EXH44_RS02005 (EXH44_02020) fabZ 427739..428146 (-) 408 WP_244238750.1 3-hydroxyacyl-ACP dehydratase FabZ -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52718.51 Da        Isoelectric Point: 4.9814

>NTDB_id=351749 EXH44_RS01975 WP_162856043.1 422726..424111(+) (pilB) [Actinobacillus indolicus strain AIFJ1607]
MSQYTVCEQQTERIFDISPSRWQQNCEEKELLLRYLAVPVQETEHKLWLAVDDENNLTACEIFAFMTHKQIEPVVIASDE
LKYLLNALSPEQQPLYEESELAFAEQEQEQLNLSDPIIQLLDNLFKFCLTQNASDIHIEPRKQKLIIRLRIDGVLHLYKS
LSIQLASRLISRIKLLAKLDISELRQPQDGQFSFTTALADTLDFRVSSLPTIYGEKLVLRLQKNKPTSFDFLQLGFNPQQ
QTTLLNALKQPQGLILVTGPTGSGKSITLYSALNYLNQMDKHILTAEDPVEIEIDGLIQTQVNQSINLDFSQLLRTFLRQ
DPDIIMLGEIRDEESAKMALRASQTGHLVLSTLHTNDALLAVERLLQLGIQEYEIQNSLLLVIAQRLVRKLCKKCRGIGC
DECYQGYKGRIGIYQCLSRTAKKFDKQTAYLDFSSLRESAKQKIEQQLTNEIEVDRVLGDE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=351749 EXH44_RS01975 WP_162856043.1 422726..424111(+) (pilB) [Actinobacillus indolicus strain AIFJ1607]
ATGTCCCAATACACCGTCTGCGAACAACAAACCGAACGCATTTTTGATATATCGCCTAGCCGTTGGCAACAAAATTGCGA
AGAAAAAGAGCTGTTATTGCGTTATCTTGCCGTTCCTGTGCAAGAAACTGAGCATAAATTATGGCTTGCCGTTGATGATG
AAAACAATCTCACCGCTTGCGAAATCTTTGCTTTTATGACCCATAAGCAGATTGAACCTGTGGTAATTGCTTCTGATGAG
CTTAAATACCTGCTTAACGCACTCTCGCCTGAGCAACAACCCCTTTATGAAGAGAGCGAACTGGCTTTCGCAGAACAAGA
GCAAGAACAGCTCAACTTAAGCGATCCGATTATTCAACTGCTCGATAATCTTTTTAAATTCTGTTTAACCCAAAATGCGT
CTGATATTCATATTGAACCACGCAAGCAAAAATTGATTATCCGCTTACGCATTGACGGTGTTTTGCACCTGTATAAAAGC
CTGTCTATTCAACTGGCAAGCCGACTTATTTCACGGATAAAACTGCTGGCGAAGCTAGATATTAGTGAATTACGCCAGCC
ACAAGATGGGCAATTTAGCTTTACTACCGCCTTGGCGGACACCCTTGATTTTCGGGTATCTAGCCTGCCGACGATCTACG
GCGAAAAACTGGTATTGCGCCTACAAAAAAACAAACCAACCAGTTTTGATTTTCTGCAACTTGGCTTTAATCCACAACAG
CAGACCACACTACTCAACGCCTTGAAACAACCGCAAGGGCTGATTTTAGTGACAGGACCAACGGGCAGTGGCAAAAGTAT
CACCTTGTATAGTGCCTTAAACTACCTCAACCAAATGGATAAACATATTCTCACCGCCGAAGATCCTGTGGAAATTGAGA
TTGACGGCTTAATTCAAACCCAAGTCAATCAAAGTATTAACCTTGATTTTAGCCAGCTATTACGCACCTTTCTACGCCAA
GATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAGAGTGCAAAAATGGCATTAAGAGCAAGCCAGACGGGGCA
TTTAGTCCTTTCAACCCTGCATACCAATGACGCACTATTAGCGGTCGAACGTTTGTTACAACTGGGTATTCAAGAGTATG
AAATCCAAAATTCACTACTGTTAGTCATTGCACAACGCCTTGTGCGTAAGCTCTGCAAAAAGTGTCGTGGCATAGGCTGT
GATGAGTGCTATCAAGGCTATAAAGGGCGGATCGGGATTTACCAATGTTTAAGTAGAACTGCAAAAAAATTCGATAAACA
GACCGCTTACTTGGACTTTTCCAGCTTACGAGAGAGTGCTAAACAAAAAATTGAACAACAACTAACCAACGAAATCGAAG
TGGATAGGGTATTAGGCGATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P7CE13

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

96.746

100

0.967
  pilB Haemophilus influenzae Rd KW20

57.363

98.698

0.566
  pilB Haemophilus influenzae 86-028NP

56.645

99.566

0.564
  pilB Vibrio cholerae strain A1552

38.755

100

0.419
  pilB Vibrio parahaemolyticus RIMD 2210633

39.917

100

0.416
  pilB Vibrio campbellii strain DS40M4

39.917

100

0.416
  pilB Acinetobacter baylyi ADP1

36.634

100

0.401
  pilB Acinetobacter baumannii D1279779

35.992

100

0.401
  pilB Legionella pneumophila strain ERS1305867

40.682

95.445

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.085

100

0.388
  pilF Neisseria gonorrhoeae MS11

36.842

100

0.38

Multiple sequence alignment